Some Properties of the Beta-Lactamase Genes in Staphylococcus epidermidis

Baldwin, Jack N.; Strickland, Robert H.; Cox, Marilyn F.

doi:10.1128/aem.18.4.628-630.1969

Cited by 11 publications

(7 citation statements)

References 6 publications

(7 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Naturally, the isolated Staphylococcus epidermidis strains can produce β-lactamase [ 22 ], and the result from enzyme assay found that quercetin had an inhibitory activity against β-lactamase type IV from E. cloacae. Hence, these results provide evidence that one mode of action of quercetin against ARSE may involve in β-lactamase inhibition and may cause the resistance of bacterial strains to its sensitivity to an antibiotic [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The synergy and mode of action of quercetin plus amoxicillin against amoxicillin-resistant Staphylococcus epidermidis

Siriwong

Teethaisong

Thumanu

et al. 2016

BMC Pharmacol Toxicol

View full text Add to dashboard Cite

BackgroundStaphylococcus epidermidis is one of the most multiple resistances to antibiotics in the recent years. Therefore, practically-prescribed antibiotics in the treatment of these strains are not effective. Plant-derived antibacterial is one of the most interesting sources of new therapeutics. The present study was to investigate antibacterial, synergy and modes of action of quercetin and amoxicillin against amoxicillin-resistant Staphylococcus epidermidis (ARSE).MethodsThe MICs, checkerboard assay, viability curves, cytoplasmic membrane (CM) permeability, enzyme assay, transmission electron microscopy, confocal microscopy and FT-IR microspectroscopy measurement was performed.ResultsThe MICs of amoxicillin, penicillin, quercetin and kaempferol against all ARSE strains were 16, 200, 256-384 and >1024 μg/mL respectively. Synergistic effects were exhibited on amoxicillin plus quercetin and penicillin plus kaempferol against these strains at FIC index 0.50 and <0.38 respectively. The synergistic activity of quercetin plus amoxicillin was confirmed by the viable count. This combination increased CM permeability, caused marked morphological, peptidoglycan and cytoplasmic membrane damage, increased protein amide I and II, but decreased fatty acid in bacterial cells. The quercetin had an inhibitory activity against β-lactamase.ConclusionsSo, these findings are the first report that quercetin has the synergistic effect with amoxicillin against ARSE via four modes of actions, inhibit peptidoglycan synthesis and β-lactamases activity, increase CM permeability and protein amide I and II but decrease fatty acid in bacterial cells. Of course, this flavonol has the dominant potential to develop a brand-new collateral phytochemical agent plus amoxicillin to treat ARSE. Future work should focus on the bioavailability, efficacy and toxicity in animal and human studies, as well as, the synergistic effect on blood and tissue should be evaluated and achieved.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Many isolated cultures of Staphylococcus epidermidis strains produced beta-lactamase [ 22 ]. Bacteria have produced β-lactamase enzymes that can inactivate β-lactam antibiotics by hydrolyzing the peptide bond of the β-lactam ring rendering the antibiotic ineffective [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

The synergy and mode of action of quercetin plus amoxicillin against amoxicillin-resistant Staphylococcus epidermidis

Siriwong

Teethaisong

Thumanu

et al. 2016

BMC Pharmacol Toxicol

View full text Add to dashboard Cite

show abstract

“…Small inocula (105 cells per ml) of penicillinase-positive (P+) strains were incubated with constant shaking at 37 C for 18 hr in YETS broth containing 0.002% SDS (Matheson, Coleman and Bell, Norwood, Ohio). Colonies arising from cells which had lost the penicillinase plasmid were detected 1y the method of Baldwin et al (1).…”

Section: Methodsmentioning

confidence: 99%

Loss of the Penicillinase Plasmid After Treatment of Staphylococcus aureus with Sodium Dodecyl Sulfate

Sonstein

Baldwin

1972

J Bacteriol

View full text Add to dashboard Cite

Two strains of Staphylococcus aureus (PC1 and 196E), when grown in medium containing 0.002% sodium dodecyl sulfate (SDS), showed from 96.1 to 100% loss of the ability to produce penicillinase. Resistance to cadmium and zinc was lost concomitantly with the ability to produce penicillinase. A comparison of the rate of curing by SDS with the curing effects exerted by elevated temperature and ethidium bromide suggested that SDS is a more effective plasmid curing agent for susceptible strains of S. aureus than methods in use at present.

show abstract

“…Methicillin-resistant (mec r) and tetracycline-resistant (tetr) colonies were scored on CH plates supplemented with 12.5 ug of methicillin per ml for mecr and with 15 ,ug of tetracycline per ml for tetr. Induced penicillinase synthesis by single colonies was detected as described by Baldwin et al (2). Drug susceptibilities of all strains which appeared to have lost antibiotic resistance were retested by the disk method (6).…”

Section: Sn521mentioning

confidence: 99%

Isolation and characterization of a plasmid involved with enterotoxin B production in Staphylococcus aureus

Z¹,

Hertman²,

Sarid³

1977

J Bacteriol

View full text Add to dashboard Cite

Genetic analysis and molecular characterization of plasmid deoxyribonucleic acid (DNA) was performed in a toxigenic isolate ofStaphylococcus aureus strain DU4916. Elimination, transduction, and transformation experiments provided us with a series of derivatives similar except for the presence or absence of genes mediating resistance to penicillin (penr), methicillin (mecr), and tetracycline (tetr) and enterotoxin type B (SEB) production (entB+). The derivatives were examined for the presence of a plasmid species which encodes for SEB production. Two distinct species of covalently closed circular DNA of about 2.8 x 106 and 0.75 x 106 daltons were identified in an ethidium bromide-cured, penicillinase-negative (pens) isolate, SN109 (mecr tetr emtB+). Further segregation of either methicillin resistance or tetracycline resistance or of both together resulted in the loss of SEB production and the disappearance of both plasmids. Transduction from strain SN109 showed that determinants for tetracycline resistance are carried by the 2.8 x 106f-dalton plasmid. Transformation with covalently closed circular DNA from strain SN109 yielded mecs tetr entBtransformants harboring the tetracycline resistance plasmid alone and mecr tetr entB+ transformants harboring both the tetracycline resistance and the 0.75 x 106dalton plasmid. Further segregation of methicillin resistance in transformants was not associated with any change in plasmid DNA. The results indicate that a genetic determinant for SEB production is carried by the 0.75 x 106-dalton plasmid. It is possible, however, that this plasmid cannot be maintained in the host independently from the tetracycline resistance plasmid. Methicillin resistance in the strains examined could not be ascribed to any of the covalently closed circular DNA components resolved in strain DU4916.

show abstract

Some Properties of the Beta-Lactamase Genes in Staphylococcus epidermidis

Cited by 11 publications

References 6 publications

The synergy and mode of action of quercetin plus amoxicillin against amoxicillin-resistant Staphylococcus epidermidis

The synergy and mode of action of quercetin plus amoxicillin against amoxicillin-resistant Staphylococcus epidermidis

Loss of the Penicillinase Plasmid After Treatment of Staphylococcus aureus with Sodium Dodecyl Sulfate

Isolation and characterization of a plasmid involved with enterotoxin B production in Staphylococcus aureus

Contact Info

Product

Resources

About