Some possibilities of representing microcirculation in human spleen

Gálfiová, Paulína; Varga, Ivan; Kopáni, Martin; Michalka, Peter; Michalková, Jana; Jakubovský, J; Polák, Štefan

doi:10.2478/s11756-009-0193-5

Cited by 3 publications

(5 citation statements)

References 19 publications

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“…The ultrathin sections were contrasted with uranyl acetate and lead citrate. For SEM we identically postfixed the tissue, after dehydration we dried it at the critical point of CO2 (CPD 020 Balzers Union) and galvanized it with gold for 2.5 min at the pressure of 2500 Pa and the current of 50 mA in the apparatus SCD 030 Balzers Union, the anode-cathode distance being 55 mm Gálfiová et al 2009). …”

Section: Methodsmentioning

confidence: 99%

Options for histological study of the structure and ultrastructure of human urinary bladder epithelium

et al. 2012

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Abstract:The urothelium lines all urinary passages, with exception of the distal portions of the urethra. For the first time the structure of the human bladder was described by Leonardo Da Vinci in 15 th century, however, the exact ultrastructure and function of the bladder's epithelium have not been fully understood. The aim of our study was to investigate the structure of normal human urinary bladder epithelium with methods of classical histology, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). We obtained biopsies from non-tumor areas from the human urinary bladder of tumor-bearing patients during transurethral resections of these tumours in general or spinal anaesthesia. Totally we investigated biopsies from 20 patients, 16 males and 4 females. The mean age of this group of patients was averaged 66.5 years. The urothelium is comprised of three cell types including polyhedral basal cells, piriform intermediate cells, and superficial umbrella cells. In human urinary bladder epithelium we found a direct connection between intermediate cells and the basement membrane. These thin cytoplasmic projections are detectable not only on slides for light microscopy (semithin sections), but also in transmission electron-micrographs. In semi-thin sections we found also direct connections between superficial umbrella cells and basement membrane. These connections we were not able to verify via transmission electronmicroscopy. Nevertheless our results show that the human urinary bladder urothelium is a special type of pseudostratified epithelium and each cell has a thin cytoplasmic projection with a direct contact with basement membrane.

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Section: Methodsmentioning

confidence: 99%

Options for histological study of the structure and ultrastructure of human urinary bladder epithelium

et al. 2012

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“…We note that as the primary deformation mode is bending and that since the maximum bending deflection would scale as L 3 EC /D 4 EC , the expected EC deflections would be comparable to those with the somewhat uncertain "averages" of LEC ∼ 9µm and DEC ∼ 1.8 − 2µm. We also note the sinus image of Gálifiová et al [39] in Fig. 1e that suggested that the EC length may be larger than the erstwhile estimated average of 9µm.…”

Section: Model and Simulation Detailsmentioning

confidence: 54%

“…1c and suggested by Fig. 1e taken from Gálfiová et al [39]. Drenckhahn and Wagner [35] report spacings, based on optical microscopy, of individual ring fibers in the human spleen in the range 4 − 5µm (see their Figs.…”

Section: Accepted Manuscriptmentioning

confidence: 94%

“…Simulations were carried out using our WSM model and a model in which L EC = 8µm; EC lengths of O(∼ 8µm) are suggested by the SEM images of Fujita [33] and Gálifiová et al [39], discussed above. A range of physiological relevant values for cell mechanical properties and driving pressures were used to explore the possible range of forecasted IES calibers, specifically to assess what is believed to be the observed range of O(0.8 − 1.5µm).…”

Section: Simulation Goals and Expectationsmentioning

confidence: 99%

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Erythrocyte flow through the interendothelial slits of the splenic venous sinus

Dao

Macdonald

Asaro

2021

Biomech Model Mechanobiol

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“…The blood elements with undisturbed elasticity of cell membrane subsequently return to the blood circulation by penetrating through the small openings in the walls of specialized capillaries (splenic venous sinuses) (ref. [7][8][9][10] ). The altered erythrocytes in hereditary spherocytosis are vulnerable to splenic sequestration and subsequent lysis and cause the characteristics of this disease such as jaundice, anemia, splenomegaly, and cholelithiasis.…”

Section: Introductionmentioning

confidence: 99%

First experience with single incision laparoscopic surgery in Slovakia: Concomitant cholecystectomy and splenectomy in an 11-year-old girl with hereditary spherocytosis

Cingel¹,

Zabojnikova²,

Kurucova³

et al. 2014

BIOMED PAP

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Aims.Hereditary spherocytosis is an autosomal dominant inheritance disorder of the red blood cell membrane characterized by the presence of spherical-shaped erythrocytes (spherocytes) in the peripheral blood. The main clinical features include haemolytic anemia, variable jaundice, splenomegaly and cholelithiasis caused by hyperbilirubinemia from erythrocyte hemolysis. Splenectomy does not solve the congenital genetic defect but it stops pathological hemolysis in the enlarged spleen. If gallstones are present, it is appropriate to perform cholecystectomy at the time of splenectomy, although the patient has symptoms of gall bladder disease. We present the case of single incision laparoscopic surgical (SILS) concomitant splenectomy and cholecystectomy performed with conventional laparoscopic instruments in an 11-year-old girl with the diagnosis of hereditary spherocytosis. Methods. A 2 -3 cm umbilical incision was used for the placement of two 5 mm trocars and one 10 mm flexible videoscope. Conventional laparoscopic dissector, grasper, Ligasure, Harmonic Ace and hemoclips were the main tools during surgical procedure. We prefer Single Incision Laparoscopic Surgery Foam Port (Covidien) as the single umbilical device for introduction into the abdominal cavity. First, we performed cholecystectomy, then the gallbladder was put aside over the liver and after that we peformed splenectomy. To remove the detached spleen and gallbladder, a nylon extraction bag is introduced through one of the port sites. The spleen is than morcellated in the bag with forceps and removed in fragments. After that we removed them and the umbilical fascial incision was closed. Result. Splenectomy is the only effective therapy for this disorder and often it is performed in combination with cholecystectomy. Conventional surgery requires a wide upper abdominal incision for correct exposure of the gallbladder and spleen. Our experience shows that SILS splenectomy and cholecystectomy is feasible even in young children and despite the small number of cases in the world, we consider the combined laparoscopic approach safe and effective for the treatment of hereditary spherocytosis. Conclusion. According to actually published guidelines, the laparoscopic approach to concomitant splenectomy and cholecystectomy is recommended, but it depends on the availability of appropriately trained surgeons and suitable equipment.

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Some possibilities of representing microcirculation in human spleen

Cited by 3 publications

References 19 publications

Options for histological study of the structure and ultrastructure of human urinary bladder epithelium

Options for histological study of the structure and ultrastructure of human urinary bladder epithelium

Erythrocyte flow through the interendothelial slits of the splenic venous sinus

First experience with single incision laparoscopic surgery in Slovakia: Concomitant cholecystectomy and splenectomy in an 11-year-old girl with hereditary spherocytosis

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