Some physiological properties of an Italian isolate of “microthrix parvicella”

Tandoi, Valter; Rossetti, Simona; Blackall, Linda L.; Majone, Mauro

doi:10.2166/wst.1998.0567

Cited by 34 publications

(61 citation statements)

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“…However, the observed trends are in agreement with pure culture studies where "Ca. Microthrix parvicella" seem to be able to reduce nitrate to nitrite (40). This metabolic feature (anaerobic storage of long-chain fatty acid, followed by growth with oxygen or nitrate) can probably explain why "Ca.…”

Section: Discussionmentioning

confidence: 99%

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Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of ¹⁴ CO ₂

Hesselsøe

Nielsen

Roslev

et al. 2005

Appl Environ Microbiol

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14 CO 2 assimilation. This is a metabolic feature that has not been described previously for filamentous bacteria. Such information could not have been derived by using the traditional MAR procedure, whereas the new HetCO 2 -MAR approach differentiates better between substrate uptake and substrate metabolism that result in growth. The HetCO 2 -MAR results were supported by stable isotope analysis of 13 C-labeled phospholipid fatty acids from activated sludge incubated under aerobic and anaerobic conditions in the presence of 13 CO 2 . In conclusion, the novel HetCO 2 -MAR approach expands the possibility for studies of the ecophysiology of uncultivated microorganisms.

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Section: Discussionmentioning

confidence: 99%

“…Some filamentous bacteria in activated sludge, including "Candidatus Microthrix parvicella" are extremely difficult to isolate (7,40). "Ca.…”

mentioning

confidence: 99%

Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of ¹⁴ CO ₂

Hesselsøe

Nielsen

Roslev

et al. 2005

Appl Environ Microbiol

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“…So far only a few studies on M. parvicella pure cultures have been reported (Slijkhuis 1983;Slijkhuis et al, 1984;Seviour et al, 1994;Blackall et al, 1996;Tandoi et al, 1998, Rossetti et al, 2002, due to difficulties in maintaining the micro-organism in culture.…”

Section: Introductionmentioning

confidence: 99%

Effect of solids retention time on <i>Microthrix parvicella</i> growth

Noutsopoulos¹,

Mamais²,

Andreadakis³

2007

WSA

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The objective of this study was to evaluate the effect of solids retention time (SRT) on M. parvicella growth and to calculate growth kinetic parameters of this filamentous species. Bench-scale continuous-flow experiments showed that M. parvicella growth can be significantly suppressed at an SRT of lower than 5.7 d for temperatures of between 14 and 18 o C. According to the continuous-flow experiments the maximum sludge age for the avoidance of filamentous foaming problems caused by M. parvicella is 6 d for temperatures lower than 18 o C. At this sludge age M. parvicella loses its hydrophobicity and therefore its foaming potential. However, even lower SRTs are required in order to achieve a significant suppression of its growth. At SRT values of less than 5.7 d M. parvicella initially forms a shorter filament (< 150 μm) with clear spaces inside filaments and variable Gram stain reaction and eventually is eliminated from activated sludge biocoenosis. According to kinetic studies presented in this paper, M. parvicella is a slow growing bacterium with a low maximum specific growth rate of 0.67 1/d and 0.53 1/d under aerobic and anoxic conditions respectively. Maintenance energy requirements of M. parvicella were found to be significantly lower than the maintenance energy of floc forming micro-organisms as well as other filamentous species, thus providing the micro-organism with a significant advantage under starvation conditions prevailing at the majority of the extended aeration activated sludge systems.

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“…Estimation of the growth of CT3 was carried out every 10-20 h by using the total extended filament length (TEFL) technique, determined by a computerized microscopic analytical method with a Axioskop microscope and a Kontron Electronic Imaging Analysis system KS100 (both from Zeiss) (Tandoi et al, 1998). Total filament length in 10 ml was measured and results were expressed as mm filaments (ml of liquid)…”

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Phylogenetic and physiological characterization of a heterotrophic, chemolithoautotrophic Thiothrix strain isolated from activated sludge

Rossetti

Blackall

Levantesi

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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The sheathed filamentous bacterium known as strain CT3, isolated by micromanipulation from an activated sludge treatment plant in Italy, is a member of the genus Thiothrix in the c-Proteobacteria according to 16S rDNA sequence analysis. The closest phylogenetic neighbours of strain CT3 are strains I and Q T , which were also isolated from activated sludge and belong to the species Thiothrix fructosivorans. These strains have respectively 99?2 and 99?4 % similarity to CT3 by 16S rDNA sequence comparison. CT3 shows 63-67 % DNA-DNA hybridization with strain I, which is the only currently viable strain of T. fructosivorans. CT3 is the second strain in the genus Thiothrix that has been shown to be capable of growing autotrophically with reduced sulfur compounds as the sole energy source; autotrophy was also confirmed in strain I. The first reported chemolithoautotrophic isolate of this genus was a strain of 'Thiothrix ramosa' that was isolated from a hydrogen sulfide spring and is morphologically distinguishable from all other described strains of Thiothrix, including CT3. CT3 is an aerobic organism that is non-fermentative, not capable of denitrification and able to grow heterotrophically. Autotrophy in the genus Thiothrix should be investigated more fully to better define the taxonomy of this genus.

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Some physiological properties of an Italian isolate of “microthrix parvicella”

Cited by 34 publications

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Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of ¹⁴ CO ₂

Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of ¹⁴ CO ₂

Effect of solids retention time on <i>Microthrix parvicella</i> growth

Phylogenetic and physiological characterization of a heterotrophic, chemolithoautotrophic Thiothrix strain isolated from activated sludge

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Some physiological properties of an Italian isolate of “microthrix parvicella”

Cited by 34 publications

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Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of 14 CO 2

Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of 14 CO 2

Effect of solids retention time on <i>Microthrix parvicella</i> growth

Phylogenetic and physiological characterization of a heterotrophic, chemolithoautotrophic Thiothrix strain isolated from activated sludge

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Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of ¹⁴ CO ₂

Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of ¹⁴ CO ₂