Some Observations on the Regeneration of Smooth Muscle in the
Repaired Urinary Bladder of the Rabbit

Gorham, S. D.; French, D. A.; Shivas, A A; Scott, Robert N.

doi:10.1159/000471636

Cited by 38 publications

(14 citation statements)

References 2 publications

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“…While signs of nerve regeneration were observed, none of the animals regained full function after 2 months. While smooth muscle has been reported to be able to regenerate following injury (17,18), this process may take 2–3 months for the healing process to complete even in small lesions. It is unclear what the effect of a denuded segment of ureter would have over a longer healing period.…”

Section: Discussionmentioning

confidence: 99%

Feasibility of Catheter-Directed Intraluminal Irreversible Electroporation of Porcine Ureter and Acute Outcomes in Response to Increasing Energy Delivery

Srimathveeravalli

Silk

Wimmer

et al. 2015

Journal of Vascular and Interventional Radiology

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Purpose To evaluate the feasibility of focal intraluminal irreversible electroporation (IRE) in the ureter with a novel electrode catheter and to study the treatment effects in response to increasing pulse strength. Material and Methods Five IRE treatment settings were each evaluated twice for the ablation of normal ureter in five Yorkshire pigs (1–4 ablations/animal, total of 10 ablations) using a prototype device under ultrasound and fluoroscopic guidance. Animals received either unilateral or bilateral treatment, limited to a maximum of two ablations in any one ureter. Treatment was delivered with increasing pulse strength (1000V–3000V; increments of 500V) while keeping the pulse duration (100 µseconds) and number of pulses (90) constant. Ureter patency was assessed immediately following treatment with an antegrade ureteropyelography. Animals were sacrificed within 4 hours following treatment, and treated urinary tract was harvested for histopathologic analysis using hematoxylin and eosin (H&E) and Masson’s Trichrome (MT) stains. Results IRE was successfully performed in all animals without evidence of ureteral perforation. H&E analysis of IRE treatments demonstrated full thickness ablation at higher field strengths (mucosa to the adventitia). MT stains showed preservation of connective tissue at all field strengths. Conclusion Intraluminal catheter directed IRE ablation is feasible and produces full thickness ablation of normal ureters. There was no evidence of lumen perforation even at the maximum voltages evaluated in the study.

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Section: Discussionmentioning

confidence: 99%

Feasibility of Catheter-Directed Intraluminal Irreversible Electroporation of Porcine Ureter and Acute Outcomes in Response to Increasing Energy Delivery

Srimathveeravalli

Silk

Wimmer

et al. 2015

Journal of Vascular and Interventional Radiology

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“…Bladder muscle tissue is less likely to regenerate in a normal fashion. Both the urothelial and muscle ingrowth are believed to be initiated from the edges of the normal bladder toward the region of the free graft 29,30 . Invariably, however, contracture or resorption of the graft has been evident.…”

Section: Discussionmentioning

confidence: 99%

De novo reconstitution of a functional mammalian urinary bladder by tissue engineering

et al. 1999

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Human organ replacement is limited by a donor shortage, problems with tissue compatibility, and rejection. Creation of an organ with autologous tissue would be advantageous. In this study, transplantable urinary bladder neo-organs were reproducibly created in vitro from urothelial and smooth muscle cells grown in culture from canine native bladder biopsies and seeded onto preformed bladder-shaped polymers. The native bladders were subsequently excised from canine donors and replaced with the tissue-engineered neo-organs. In functional evaluations for up to 11 months, the bladder neo-organs demonstrated a normal capacity to retain urine, normal elastic properties, and histologic architecture. This study demonstrates, for the first time, that successful reconstitution of an autonomous hollow organ is possible using tissue-engineering methods.

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“…The tracing of seeded myoblasts using different cell markers, such as green fluorescent protein, was not performed in this study, but should be considered in future studies to investigate the contribution of the transplanted cells in esophageal tissue remodeling. Finally, transmutation of fibroblast progenitor cells 34 and action of marrow derived mesenchymal stem cells, 35 suggested by some authors, are still worth exploring.…”

Section: Article In Pressmentioning

confidence: 99%

Circumferential esophageal replacement using a tube-shaped tissue-engineered substitute: An experimental study in minipigs

Poghosyan

Sfeir

Michaud

et al. 2015

Surgery

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Some Observations on the Regeneration of Smooth Muscle in the Repaired Urinary Bladder of the Rabbit

Cited by 38 publications

References 2 publications

Feasibility of Catheter-Directed Intraluminal Irreversible Electroporation of Porcine Ureter and Acute Outcomes in Response to Increasing Energy Delivery

Feasibility of Catheter-Directed Intraluminal Irreversible Electroporation of Porcine Ureter and Acute Outcomes in Response to Increasing Energy Delivery

De novo reconstitution of a functional mammalian urinary bladder by tissue engineering

Circumferential esophageal replacement using a tube-shaped tissue-engineered substitute: An experimental study in minipigs

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