Some observations on the intrinsic nervous mechanism in Hirschsprung's disease

Frigo, G. M.; Tacca, M. Del; Lecchini, Sergio; Crema, A.

doi:10.1136/gut.14.1.35

Cited by 67 publications

(16 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The second hypothesis has been supported by physiologic examinations [14] and an immunohistochemical study about peptidergic neuron [15] and nitric oxide neuron [16]. The presence of mass contractions occurring either spontaneously or after the distension of the aganglionic segments was noted both in vivo and in vitro.…”

Section: Discussionmentioning

confidence: 92%

Intracellular calcium mobilization of the aganglionic intestine in the endothelin B receptor gene –deficient rat

Nakatsuji

Ieiri

Masumoto

et al. 2007

Journal of Pediatric Surgery

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 92%

Intracellular calcium mobilization of the aganglionic intestine in the endothelin B receptor gene –deficient rat

Nakatsuji

Ieiri

Masumoto

et al. 2007

Journal of Pediatric Surgery

View full text Add to dashboard Cite

“…The enteric nerves of the muscle strips were electrically stimulated via a clip (Selfin clip; ME Commercial) held at both ends and connected to a stimulator (Model ME-6052; ME Commercial). A low frequcy, 5 Hz, which is considered to stimulate only nerve fibers, 9 with a duration of 0.5 ms, voltage of 50V, and stimulation time of 30s was employed. The stimulation was given according to the square-wave repetitive method (Fig.…”

Section: Methodsmentioning

confidence: 99%

Role of non-adrenergic non-cholinergic inhibitory nerves in the colon of patients with ulcerative colitis

1998

View full text Add to dashboard Cite

The cause of impaired colonic motility in patients with ulcerative colitis (UC) is unknown. The non-adrenergic non-cholinergic (NANC) inhibitory nervous system is one of the most important factors in the enteric nervous system of human gut. To assess the physiological significance of NANC inhibitory nerves in the colon of patients with UC, we investigated the enteric nerve responses of colonic tissues from patients with this disease. Colonic tissues were obtained from the lesional sigmoid colons of six patients with UC. Normal sigmoid colonic tissues obtained from ten patients with colonic cancer were used as controls. A mechanographic technique was used to evaluate in-vitro muscle responses to the electrical field stimulation (EFS) of adrenergic and cholinergic nerves before and after treatment with various autonomic nerve blockers. NANC inhibitory nerves were found to act on both normal colon and the lesional colon of patients with UC, but colon with UC was more strongly innervated by NANC inhibitory nerves than was the normal colon. These findings suggest that NANC inhibitory nerves play an important role in the impaired motility observed in the colon of patients with UC.

show abstract

“…Thus, it was suggested that the eholinergic excitatory and non-adrenergic inhibitory nerves do not innervate the smooth museie cells in the aganglionic colons ofHirschsprung's disease. The absence of a non-adrenergic inhibitory nerves in the aganglionic colon has been previously shown by Frigo et al (15), Richardson (30), Bywaler and Tay/or (13), and Kubota et al (25). The non-adrenergic inhibitory nerves are considered to be one ofthe intrinsic nerves connecting myenteric ganglia to smooth museie cells (I I).…”

Section: Discussionmentioning

confidence: 62%

“…This helps to explain the hypothesis that the aganglionic colon in Hirschsprung's disease is spastic. Furthermore, physiological and pharmacological studies on the aganglionic colon in Hirschsprung's disease were already carried out by many workers (13,15,18,25,27,30,31,34,38). However, there are still contradictions regarding the role of these proliferating extrinsic nerve fibres.…”

Section: Introductionmentioning

confidence: 99%

Electrophysiological and Pharmacological Study on Innervation of the Aganglionic Colon in Hirschsprung's Disease of Human and Murine Model

Okasora¹,

Okamoto²

1986

Eur J Pediatr Surg

View full text Add to dashboard Cite

A true role of extrinsic nerve fibres proliferating abundantly in the aganglionic colon of Hirschsprung's disease has not yet been established. Electrophysiological and pharmacological studies were carried out on rectal muscle strips of piebald lethal mice and their siblings. Muscle strips of both aganglionic and normal colon obtained from fifteen patients with Hirschsprung's disease were also investigated for the purpose of determining whether these extrinsic nerve fibres innervate the smooth muscle cells in the aganglionic colon or not. Junction potentials and contractile tensions evoked by electric stimulations were recorded employing the double-sucrose gap method, and examined the effects of nerve blocking agents on junction potentials. In the normal colon, excitatory junction potentials and inhibitory junction potentials were recorded following stimulation in both man and mouse. Excitatory junction potentials were accompanied by muscle contraction and abolished by atropine or tetrodotoxin. Inhibitory junction potentials were resistant to phentolamine and propranolol, and abolished by tetrodotoxin. It was concluded that the normal colon of both mouse and man is innervated by cholinergic excitatory and non-adrenergic inhibitory nerves. In the aganglionic colon, on the other hand, neither junction potentials nor contractions were evoked by stimulations in both mouse and man. These evidences led to the conclusion that in the aganglionic colon in Hirschsprung's disease of man and murine model, there may be a deficiency in neuronal regulation.

show abstract

Some observations on the intrinsic nervous mechanism in Hirschsprung's disease

Cited by 67 publications

References 30 publications

Intracellular calcium mobilization of the aganglionic intestine in the endothelin B receptor gene –deficient rat

Intracellular calcium mobilization of the aganglionic intestine in the endothelin B receptor gene –deficient rat

Role of non-adrenergic non-cholinergic inhibitory nerves in the colon of patients with ulcerative colitis

Electrophysiological and Pharmacological Study on Innervation of the Aganglionic Colon in Hirschsprung's Disease of Human and Murine Model

Contact Info

Product

Resources

About