Some milestones in in vitro organ Toxicity Assessment. The Kidney as a Case Study

Morin, Jean Paul; Leclere, Caroline; Marouillat, Sylviane; Monteil, Christelle

doi:10.1016/0887-2333(95)00081-x

Cited by 7 publications

(1 citation statement)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is an established cell line derived from normal rat kidney (14). These cells have also GST activity (13). We, therefore, investigated the GST composition in NRK cells.…”

Section: Introductionmentioning

confidence: 99%

Purification and characterisation of the soluble glutathione S‐transferase isoenzymes in rat kidney derived NRK cells

Dierickx

Noble

1997

IUBMB Life

View full text Add to dashboard Cite

Glutathione S‐transferase (GST) enzymes are toxicologically important from many points of view. Rat kidney derived established NRK cells were mass cultured for the isolation of GST isoenzymes. These were enriched by affinity chromatography and separated by chromatofocusing and HPLC. Exactly the same major GST subunits were found in NRK cells as in the rat kidney. Strong evidence was also found for the presence of an aberrant form of GST 7‐7, as was described in rat kidney. A very good correlation between the NRK GST and rat kidney, and especially cis‐platinum treated kidney, was found. It is concluded that NRK cells can be considered as a valuable alternative tool for in vitro research of rat kidney phenomena, especially when toxicological interactions are investigated.

show abstract

“…This is an established cell line derived from normal rat kidney (14). These cells have also GST activity (13). We, therefore, investigated the GST composition in NRK cells.…”

Section: Introductionmentioning

confidence: 99%

Purification and characterisation of the soluble glutathione S‐transferase isoenzymes in rat kidney derived NRK cells

Dierickx

Noble

1997

IUBMB Life

View full text Add to dashboard Cite

show abstract

Comparative impact of cephaloridine on glutathione and related enzymes in LLC-PK1, LLC-RK1, and primary cultures of rat and rabbit proximal tubule cells

et al. 1996

View full text Add to dashboard Cite

Among kidney tubular epithelial cell types, proximal tubule cells are one of the major renal targets for xenobiotics. Several in vitro culture models have been proposed for use of proximal tubule cells for in vitro pharmacotoxicology studies. This paper reports a comparative study of the response to cephaloridine exposure of two established cell lines from pig (LLC-PK1) and rabbit (LLC-RK1) kidneys and primary cultures of rat and rabbit proximal tubule cells. These cultured cells were first compared for their levels of activity of alpha-methylglucopyranoside transport, alkaline phosphatase, succinate dehydrogenase, and NADPH cytochrome c reductase, their glutathione-dependent activity levels, and their adenylate cyclase response pattern to stimulation by PTH and AVP. The results presented show major phenotypic differences between these four cellular models. The differences observed in glutathione-dependent mechanism activities and regulation may in part be responsible for the variability of the responses of these four cellular models when exposed to cephaloridine.

show abstract

Defining the role of ECVAM in the development, validation and acceptance of alternative tests and testing strategies

Balls

1995

Toxicology in Vitro

View full text Add to dashboard Cite

Some milestones in in vitro organ Toxicity Assessment. The Kidney as a Case Study

Cited by 7 publications

References 41 publications

Purification and characterisation of the soluble glutathione S‐transferase isoenzymes in rat kidney derived NRK cells

Purification and characterisation of the soluble glutathione S‐transferase isoenzymes in rat kidney derived NRK cells

Comparative impact of cephaloridine on glutathione and related enzymes in LLC-PK1, LLC-RK1, and primary cultures of rat and rabbit proximal tubule cells

Defining the role of ECVAM in the development, validation and acceptance of alternative tests and testing strategies

Contact Info

Product

Resources

About