Some Lipid Characteristics of Normal Red Blood Cells of Different Age

Gastel, C. van; Berg, Dick J.C. van den; Gier, J. De; Deenen, L.L.M. Van

doi:10.1111/j.1365-2141.1965.tb06577.x

Cited by 109 publications

(27 citation statements)

References 17 publications

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“…Inasmuch as the major component of this fraction is phosphatidyl serine (which has a negligible turnover rate with plasma), this may be merely a reflection of lipid loss with cell aging [27,37]. Previous studies, however, have not noted significant changes in phospholipid distribution with cell aging [34].…”

Section: Discussionmentioning

confidence: 89%

Alteration of Adult Erythrocyte Lipids during In Vivo Fetal Circulation

Neerhout¹

1971

Pediatr Res

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Section: Discussionmentioning

confidence: 89%

Alteration of Adult Erythrocyte Lipids during In Vivo Fetal Circulation

Neerhout¹

1971

Pediatr Res

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“…In solutions containing 500 mOsm/liter, CC cells were about four times as viscous as AA cells. AA and CC cells were ultracentrifuged from EDTA plasma, and the viscosity of old and young cells was compared (Table II) 37 2.86 2.4 Sickled cell 34 3.10 Approx. 300 (25) CC microspherocyte 39 2.71 8.4 Crystallographic data suggest that the dimensions of the hemoglobin molecule are 64 X 55 X 50 A (53).…”

Section: Methodsmentioning

confidence: 99%

Pathogenesis of Hemolytic Anemia in Homozygous Hemoglobin C Disease*

Charache¹,

Conley²,

Waugh³

et al. 1967

J. Clin. Invest.

119

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Abstract. Hemoglobin C is less soluble than hemoglobin A in red cells, in hemolysates, and in dilute phosphate buffer. Its relative insolubility may be explained by electrostatic interactions between positively charged f36-lysyl groups and negatively charged groups on adjacent molecules. Red cells from patients with homozygous hemoglobin C (CC) disease exhibit aberrant physical properties which suggest that the cells are more rigid than normal erythrocytes. They pass through membrane filters less readily than normal red cells do, and their viscosity is higher than that of normal cells. Differences from normal cells are exaggerated if mean corpuscular hemoglobin concentration (MCHC) is increased, by suspension in hypertonic salt solution. Increased rigidity of CC cells, by accelerating their fragmentation, may be responsible for formation of microspherocytes. These small dense cells are exceptionally rigid, and probably are even more susceptible to fragmentation and sequestration. Rigidity of CC cells can be attributed to a "precrystalline" state of intracellular hemoglobin, in which crystallization does not occur, although the MCHC exceeds the solubility of hemoglobin in hemolysates.

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“…Reticulocytes may constitute a higher percentage of the erythrocytes during this period [19]. Reticulocytes have been shown to have a larger amount of total lipid, phospholipid, and cholesterol per cell [26]. Since reticulocytes also have a larger corpuscular volume than older cells, some of the characteristics of the study group may be explained by the presence of reticulocytes.…”

Section: Discussionmentioning

confidence: 95%

“…Since reticulocytes also have a larger corpuscular volume than older cells, some of the characteristics of the study group may be explained by the presence of reticulocytes. The phospholipid and phospholipid fatty acid composition of reticulocytes is similar to that of older cells, however [26]. Cord bloods containing large numbers of nucleated erythrocytes and reticulocytes (obtained from erythroblastotic infants) give values for total lipid, lipid P, and cholesterol significantly lower than those from infants with birth weights ranging from 200 to 1,200 g (total lipid = 7.11 X 10-" mg/cell; P = 1.74 X 10-" mg/cell; cholesterol = 1.86 x 10-10 mg/cell) [17].…”

Section: Discussionmentioning

confidence: 96%

Erythrocyte Lipids in Infants with Low Birth Weights

Neerhout¹

1971

Pediatr Res

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ExtractThe lipid composition of erythrocytes, obtained from the cord blood of prematurely born, low birth weight infants, was compared with that obtained from term infants. Infants weighing less than 1,200 g at birth had increased total lipids, lipid phosphorus (P), and cholesterol per cell (total lipid = 7.60 X 10~1 0 mg; lipid P = 1.80 X 10" 11 mg; cholesterol = 2.10 X 10~1 0 mg) when compared with controls (total lipid = 6.45 X 10-10 mg; lipid P = 1.54 X 10~n mg; cholesterol = 1.79 X lO" 10 mg). The lipid content per 100 ml packed cells and the percentage of total lipid as phospholipid and cholesterol did not differ significantly over the range of birth weights studied. Phospholipid fractionation showed a higher percentage of phosphatidyl choline in infants under 1,200 g birth weight (30.0% of total lipid P) than that noted in term infants (27.7% of total lipid P). The higher levels of combined phosphatidyl serine and phosphatidyl inositol noted in term infant erythrocytes (compared with normal adult cells) were observed in the low birth weight infants. Fatty acids esterified to phospholipids showed an increasing percentage of oleic acid with decreasing birth weight (infants of less than 1,200 g had 16.0% total fatty acid; infants of more than 2,500 g had 11.9% total fatty acid). SpeculationThese studies suggest that the earliest embryonic erythrocytes have the greatest membrane lipid content although the overall membrane lipid distribution does not vary greatly during fetal life.

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Some Lipid Characteristics of Normal Red Blood Cells of Different Age

Cited by 109 publications

References 17 publications

Alteration of Adult Erythrocyte Lipids during In Vivo Fetal Circulation

Alteration of Adult Erythrocyte Lipids during In Vivo Fetal Circulation

Pathogenesis of Hemolytic Anemia in Homozygous Hemoglobin C Disease*

Erythrocyte Lipids in Infants with Low Birth Weights

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