Some limits to post‐antigen generation of diversity: Failure to detect variants in clones of hapten‐specific antibody‐forming cells (AFC) developing in culture from direct AFC‐progenitor B cells
Abstract:A search was made for variants in clones of hapten-specific antibody-forming cells (AFC) arising by stimulation of mature B cells with either thymus-independent hapten-POL (polymerized bacterial flagellin) conjugates, or the polyclonal activator lipopolysaccharides. Enriched, hapten-binding B cells or unfractionated spleen cells were cultivated for 3-4 days at limiting dilution in the presence of thymus filler cells, and the AFC in each microculture well were then assayed for plaque formation on various hapten… Show more
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