Some genetic profiles in liver of Ehrlich ascites tumor-bearing mice under the stress of irradiation

Hassan, Amal I.; Ibrahim, Rasha Y.M.

doi:10.1016/j.jrras.2014.02.002

Cited by 5 publications

(4 citation statements)

References 48 publications

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“…EAC cells spread and proliferate in hepatocytes causing hepatocellular dysfunction [ 100 ], which leads to an increase in the antiapoptotic factors Bcl2 and a decrease in proapoptotic Bax gene expression [ 101 ]. As shown in Figure 9 , real-time quantitative PCR (qPCR) of liver tissue from EAC-bearing mice demonstrates that treatment with compound ( 2 ) reverses these effects, with a significant dose-dependent reduction of Bcl2 and recovery of Bax gene expression [ 102 ]. The qPCR studies revealed significant increases in the mRNA levels of both apoptotic markers, Bax and caspase 3, following treatment, as compared to untreated Ehrlich mice, with the strongest effect at higher dose ( Figure 9 ).…”

Section: Resultsmentioning

confidence: 99%

Ruthenium(II)/(III) DMSO-Based Complexes of 2-Aminophenyl Benzimidazole with In Vitro and In Vivo Anticancer Activity

et al. 2020

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New anticancer ruthenium(II/III) complexes [RuCl2(DMSO)2(Hapbim)] (1) and [RuCl3(DMSO) (Hapbim)] (2) (Hapbim = 2-aminophenyl benzimidazole) have been synthesized and characterized, and their chemotherapeutic potential evaluated. The interaction of the compounds with DNA was studied by both UV-Visible and fluorescence spectroscopies, revealing intercalation of both the Hapbim ligand and the Ru complexes. The in vitro cytotoxicity of the compounds was tested on human breast cancer (MCF7), human colorectal cancer (Caco2), and normal human liver cell lines (THLE-2), with compound (2) the most potent against cancer cells. The cytotoxic effect of (2) is shown to correlate with the ability of the Ru(III) complex to induce apoptosis and to cause cell-cycle arrest in the G2/M phase. Notably, both compounds were inactive in the noncancerous cell line. The anticancer effect of (2) has also been studied in an EAC (Ehrlich Ascites Carcinoma) mouse model. Significantly, the activity of the complex was more pronounced in vivo, with removal of the cancer burden at doses that resulted in only low levels of hepatotoxicity and nephrotoxicity. An apoptosis mechanism was determined by the observation of increased Bax and caspase 3 and decreased Bcl2 expression. Furthermore, (2) decreased oxidative stress and increased the levels of antioxidant enzymes, especially SOD, suggesting the enhancement of normal cell repair. Overall, compound (2) shows great potential as a chemotherapeutic candidate, with promising activity and low levels of side effects.

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Section: Resultsmentioning

confidence: 99%

Ruthenium(II)/(III) DMSO-Based Complexes of 2-Aminophenyl Benzimidazole with In Vitro and In Vivo Anticancer Activity

et al. 2020

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“…Fold changes in gene expression were calculated using the mean differences in ΔCt (ΔΔCt) using the following formula: fold = 2−ΔΔC. 23 , 24 …”

Section: Methodsmentioning

confidence: 99%

Curcumin nanocapsules effect in apoptotic processes, gene expression, and cell cycle on Hep-G2 cell lines

Moawad

Nasr

Osman

et al. 2023

Int J Immunopathol Pharmacol

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Objectives Curcumin has antioxidant and antiproliferative properties, and its therapeutic effect must be considered. Nanocurcumin capsules showed a potential increase against in vitro biological cancer. This study sought to determine how curcumin nanoparticles and nanocapsules affected the expression of p53, Bcl-2, Bax, and Bax in a liver cancer cell line (Hep-G2). Mechanisms of apoptosis were also examined in this cell line. Methods This study used quantitative real-time polymerase chain reaction (qRT-PCR) to analyze the p53, Bcl-2, Bax, and Caspase-3 gene pathways and to evaluate the molecular mechanisms responsible for the efficacy of curcumin nanoparticles (CNPs) and curcumin nanocapsules (CNCs) against liver cell lines. Flow cytometry was used to check for signs of apoptosis and the cell cycle. Results Curcumin nanocapsules produced by the ball milling process at 90 min significantly boosted the populations of apoptotic cells in a dose- and time-dependent manner. The mRNA expression analysis revealed that the proapoptotic Bax, Caspase-3, and the tumor suppressor gene p53 were upregulated throughout the process started by curcumin nanocapsules and decreased in the Bcl-2/Bax ratio . Conclusion This research provides a fresh understanding of the molecular mechanisms behind the liver cancer-fighting abilities of curcumin nanoparticles. Curcumin nanocapsules produced through a unique mechanical technique can be used as an anticancer agent.

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“…The sequences of specific primer of the genes used are listed in (Table 1). , Thermal cycling conditions 50°C for 2 min and 95°C for 10 min followed by 40 cycles of 95°C for 30 s, 60°C for 30 s, and 72°C for 30 s. Changes in the expression of each target gene were normalized relative to the mean critical threshold (CT) values of (β-Actin) as housekeeping gene by the ΔΔCt method [22,23].…”

Section: Determination Of the Apoptotic Parameters In The Eac Cells Amentioning

confidence: 99%

Secondary metabolites and Biological activities of Allium porrum L. attack Ehrlich ascites carcinoma in mice

Elhagrasi

Mahmoud

Foda³

et al. 2019

Egypt. J. Chem.

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A LLIUM vegetables have been recorded to be used in many medical remedies for protection and fighting diseases. The aim of the study is to investigate the phenolic compounds of methanol extract of Allium porrum L. aerial parts (MEAP) and introduce the methanolic extract as a candidate for the treatment of cancer in vivo as well as explore its antitumor mechanisms in attacking tumor cells at the molecular level. Isolation of phenolic and flavonoid compounds by column chromatography has been carried out. They were purified by Sephadex LH-20, and the structures were established with the aid of spectroscopic methods (UV, EI/MS, ESI/MS, 1 H-NMR, and 13 C-NMR spectroscopy). A total of 45 female Swiss albino mice were divided into five groups. One group represents the normal control. The four remaining groups were divided as follows: two groups were injected with Ehrlich ascites carcinoma (EAC) cells intraperitoneally and the other two were injected intramuscularly (to form solid tumor), representing the positive nontreated groups. One group from each model was administrated orally the extract at a dose of 300 mg/kg body weight 3 days weekly for 21 days after 24 h from the intraperitoneal or intramuscular injection of EAC cells. The influence of the extract on the apoptotic proteins were tested in EAC cells and in solid tumor of the treated groups compared with the nontreated ones. Eleven phenolic and flavonoid compounds were isolated from the aerial parts of methanol extract of A. porrum L., and characterized as follows: quercetin-3acid, and gallic acid. The gene expressions of caspase-3, Bax, and Bcl2 proteins and DNA fragmentation test were performed in the EAC cells. Hematological profile was conducted on mice's blood in addition to the Immuno-histochemical determination of P53 protein in solid tumors. MEAP has abundant phenolic compounds. It exhibits tumor suppressing mechanisms through the coordination between the apoptic proteins and DNA fragmentation in the EAC or solid cancer cells. MEAP is suggested to be a potent anticancer agent.

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Some genetic profiles in liver of Ehrlich ascites tumor-bearing mice under the stress of irradiation

Cited by 5 publications

References 48 publications

Ruthenium(II)/(III) DMSO-Based Complexes of 2-Aminophenyl Benzimidazole with In Vitro and In Vivo Anticancer Activity

Ruthenium(II)/(III) DMSO-Based Complexes of 2-Aminophenyl Benzimidazole with In Vitro and In Vivo Anticancer Activity

Curcumin nanocapsules effect in apoptotic processes, gene expression, and cell cycle on Hep-G2 cell lines

Secondary metabolites and Biological activities of Allium porrum L. attack Ehrlich ascites carcinoma in mice

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