Some fundamental aspects of centrifugally accelerated paper chromatography

Centrifugal Force in Paper Chromatography and Electrophoresis

“…CENTRIFUGALLY ACCELERATED PAPER CHROMATOGRAPHY Some details of the technique, and earlier modifications of the apparatus, for carrying out centrifugally accelerated chromatographic separations in rotating circular paper sheets, have been described {2, 3,6). A technique for conducting two-dimensional separations on a rotating paper disk has been published (5).…”

“…For this system the Rf values, 0.86, 0.22, and 0.64, respectively, of the dyes were essentially unaffected by changes in rotational speed of the rotor from 300 to 925 r.p.m. (6). The flow of solvent was important in developing satisfactory chromatograms; 1.2 ml.…”

Separation of Toxicologically Important Bases via Centrifugally Accelerated Paper Chromatography

“…ACID FERRIC CHLORIDE REAGENT of Zak (12) has been considered more sensitive than many other color reagents and has been used for the microdetermination of cholesterol in serum (3,11) and cerebrospinal fluid (7, 9). However, the color reaction with this reagent is less specific than previously reported classical methods, it gives occasional higher values when used directly on plasma (1,6), and it is troubled by interfering substances (3, 8). Extraction of lipide with al-cohol-acetone (4, 10) or chloroformmethanol (7, 9) is inadequate for the complete removal of color-interfering substances.…”

Chromatography

“…Paper chromatography has been accelerated by centrifugation (251,262), by combination of centrifugation with electrolysis (262), by combination with transverse high-potential electrolysis (218), and by migration at 60°C. (179, 360).…”