Some environmental factors affect growth and antibiotic production by the mycoparasite<i>Coniothyrium minitans</i>

Tomprefa, Nicola; Hill, Robert A.; Whipps, J. M.; McQuilken, M. P.

doi:10.1080/09583157.2011.575211

Cited by 12 publications

(8 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, nutrient availability (both type and concentration) is known to affect the production of antibiotics, including specific carbon sources that can affect either the transcription or translation of specific genes [65,66]. Environmental conditions, such as temperature [67] and pH [68], can also affect antibiotic activity, as can the presence of an antagonist [69]. We performed preliminary bioassays using the supernatant of AltDE1 and did not observe any obvious activity against closely related strains of A .…”

Section: Resultsmentioning

confidence: 99%

A Hybrid NRPS-PKS Gene Cluster Related to the Bleomycin Family of Antitumor Antibiotics in Alteromonas macleodii Strains

et al. 2013

View full text Add to dashboard Cite

Although numerous marine bacteria are known to produce antibiotics via hybrid NRPS-PKS gene clusters, none have been previously described in an Alteromonas species. In this study, we describe in detail a novel hybrid NRPS-PKS cluster identified in the plasmid of the Alteromonas macleodii strain AltDE1 and analyze its relatedness to other similar gene clusters in a sequence-based characterization. This is a mobile cluster, flanked by transposase-like genes, that has even been found inserted into the chromosome of some Alteromonas macleodii strains. The cluster contains separate genes for NRPS and PKS activity. The sole PKS gene appears to carry a novel acyltransferase domain, quite divergent from those currently characterized. The predicted specificities of the adenylation domains of the NRPS genes suggest that the final compound has a backbone very similar to bleomycin related compounds. However, the lack of genes involved in sugar biosynthesis indicates that the final product is not a glycopeptide. Even in the absence of these genes, the presence of the cluster appears to confer complete or partial resistance to phleomycin, which may be attributed to a bleomycin-resistance-like protein identified within the cluster. This also suggests that the compound still shares significant structural similarity to bleomycin. Moreover, transcriptomic evidence indicates that the NRPS-PKS cluster is expressed. Such sequence-based approaches will be crucial to fully explore and analyze the diversity and potential of secondary metabolite production, especially from increasingly important sources like marine microbes.

show abstract

Section: Resultsmentioning

confidence: 99%

A Hybrid NRPS-PKS Gene Cluster Related to the Bleomycin Family of Antitumor Antibiotics in Alteromonas macleodii Strains

et al. 2013

View full text Add to dashboard Cite

show abstract

“…According a different investigation, C. minitans Strain CON/M/91-08 seems to be specially adapted to produce antibiotics at a wider environmental conditions (e.g. maximum antibiotic production per unit mass occurs at 30°C when for other C. minitans production of antibiotic drops significantly (Tomprefa et al, 2011). Therefore, strain specific studies are needed to address the production of macrosphelide A under environmental conditions.…”

Section: 2mentioning

confidence: 99%

Peer review of the pesticide risk assessment of the active substance Coniothyrium minitans Strain CON/M/91‐08

Authority¹

2016

EFS2

View full text Add to dashboard Cite

The conclusions of EFSA following the peer review of the initial risk assessments carried out by the competent authorities of the rapporteur Member State, the Netherlands, and co-rapporteur Member State, Estonia, for the pesticide active substance Coniothyrium minitans CON/M/91-08 are reported. The context of the peer review was that required by Commission Implementing Regulation (EU) No 844/2012. The conclusions were reached on the basis of the evaluation of the representative uses of C. minitans CON/M/91-08 as a fungicide by overhead irrigation or incorporation for soil decontamination against Sclerotinia sclerotiorum in winter rape, lettuce, cucumber, beans, oilseed rape, sunflower or applied with the irrigation system in winter rape and lettuce. The reliable end points, appropriate for use in regulatory risk assessment are presented. Missing information identified as being required by the regulatory framework is listed. Concerns are identified.

show abstract

“…The antifungal substances are usually not observed when dual-culturing with S. sclerotiorum on PDA medium and their production can be affected by nutrient factors (McQuilken et al, 2002 ; Yang et al, 2008 ). Recent research showed that the production of antifungal substances and mycoparasitism of C. minitans are regulated by the ambient pH value (Tomprefa et al, 2011 ). Under low pH conditions, C. minitans can produce more antifungal substances, but when pH values rise, C. minitans may produce more fungal cell wall-degrading enzymes to parasitize its hosts (Zeng et al, 2014 ; Lou et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Uninterrupted Expression of CmSIT1 in a Sclerotial Parasite Coniothyrium minitans Leads to Reduced Growth and Enhanced Antifungal Ability

et al. 2017

View full text Add to dashboard Cite

Coniothyrium minitans is an important mycoparasite of Sclerotinia sclerotiorum. In addition, it also produces small amounts of antifungal substances. ZS-1TN1812, an abnormal mutant, was originally screened from a T-DNA insertional library. This mutant showed abnormal growth phenotype and could significantly inhibit the growth of S. sclerotiorum when dual-cultured on a PDA plate. When spraying the filtrate of ZS-1TN1812 on the leaves of rapeseed, S. sclerotiorum infection was significantly inhibited, suggesting that the antifungal substances produced by this mutant were effective on rapeseed leaves. The thermo-tolerant antifungal substances could specifically suppress the growth of S. sclerotiorum, but could not significantly suppress the growth of another fungus, Colletotrichum higginsianum. However, C. higginsianum was more sensitive to proteinous antibiotics than S. sclerotiorum. The T-DNA insertion in ZS-1TN1812 activated the expression of CmSIT1, a gene involved in siderophore-mediated iron transport. It was also determined that mutant ZS-1TN1812 produced hypha with high iron levels. In the wild-type strain ZS-1, CmSIT1 was expressed only when in contact with S. sclerotiorum, and consistent overexpression of CmSIT1 showed similar phenotypes as ZS-1TN1812. Therefore, activated expression of CmSIT1 leads to the enhanced antifungal ability, and CmSIT1 is a potential gene for improving the control ability of C. minitans.

show abstract

Some environmental factors affect growth and antibiotic production by the mycoparasiteConiothyrium minitans

Cited by 12 publications

References 31 publications

A Hybrid NRPS-PKS Gene Cluster Related to the Bleomycin Family of Antitumor Antibiotics in Alteromonas macleodii Strains

A Hybrid NRPS-PKS Gene Cluster Related to the Bleomycin Family of Antitumor Antibiotics in Alteromonas macleodii Strains

Peer review of the pesticide risk assessment of the active substance Coniothyrium minitans Strain CON/M/91‐08

Uninterrupted Expression of CmSIT1 in a Sclerotial Parasite Coniothyrium minitans Leads to Reduced Growth and Enhanced Antifungal Ability

Contact Info

Product

Resources

About