Some electrical properties of lipid bilayer membranes

Miyamoto, Vernon K.; Thompson, T. E.

doi:10.1016/0021-9797(67)90005-7

Cited by 56 publications

(37 citation statements)

References 23 publications

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“…The results are expressed in this manner for convenience. Moreover, it should be noted that biological membranes generally cannot support potentials in excess of 150 to 200 mV, see [26][27][28][29][30].…”

Section: Resultsmentioning

confidence: 99%

Phosphorylation without protonmotive force

Kinnally¹,

Tedeschi²

1976

FEBS Letters

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Section: Resultsmentioning

confidence: 99%

Phosphorylation without protonmotive force

Kinnally¹,

Tedeschi²

1976

FEBS Letters

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“…The cell in which membranes were formed was similar to that described by Miyamoto and Thompson (1967). The membrane support was 1/32 inch plexiglas with a 1.3 mm diameter orifice.…”

Section: Ce//smentioning

confidence: 99%

“…Membrane resistances were determined with a circuit similar to that described by Miyamoto and Thompson (1967). Unless otherwise noted, the potential across the membrane was held at 20 mV.…”

Section: Electrical Measurementsmentioning

confidence: 99%

“…It has also been demonstrated that bilayer resistances are lowered by detergents (Seufert, 1965), oxidative phosphorylation uncouplers (Hopfer, Lehninger & Thompson, 1968;Lieberman & Topaly, 1968) and antigen-antibody complexes (Del Castillo, Rodriquez, Romero & Sanchez, 1966;Barfort, Arguilla &Vogelhut, 1968). The present investigation is based on the finding by Miyamoto and Thompson (1967) that ferric ion produces a large decrease in bilayer resistance. Since the modifying agent in this case is a cation, the system appeared to be simple enough that further study was warranted.…”

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confidence: 99%

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Properties of lipid bilayer membranes separating two aqueous phases: The effects of Fe+3 on electrical properties

MacDonald

Thompson

1972

J. Membrain Biol.

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Ferric ion has been found to alter the electrical properties of lecithincholesterol-decane bilayer membranes. Within minutes after the addition of microgram quantities of FeCl3 to the ambient aqueous phase, the resistance of the membrane falls by a factor of 10(5) to 10(6). No change in capacitance is observed. The resistance change is obtained with membranes made from synthetic lecithin (fully saturated fatty acids) as well as by those formed from egg lecithin. The conductance of the modified membrane exhibits both time and voltage dependent behavior; the time dependence of the current is similar to that of an inductance, and the voltage dependence of the current is exponential. Concomitant with the resistance change, the modified membrane becomes permselective, passing chloride almost to the complete exclusion of sodium. Anion selectivity can be converted to cation selectivity by the subsequent addition of certain chelating agents. Area-conductance measurements show the resistance change occurs in the thin film. The addition of a reducing agent causes the effect of the ferric ion to be reversed, and the conductance returns to that characteristic of unmodified membranes. When ferric ion is added to only one side of the membrane, the system rectifies with current ratios of up to 20∶1. It is concluded that the alteration of membrane properties owes its origin to the hydrolysis of membrane-bound ferric ion. The interaction of ferric ion with aqueous dispersions of lecithin has been investigated by several techniques, and evidence is presented that the dispersions bind charged species of iron and that this charge diminishes under conditions where iron hydrolysis occurs.

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“…This was not surprising since artificial bilayer membranes made from phosphatidylserine, phosphatidylglycerol (17), or a chloroformmethanol solution of egg phosphatidylcholine and tetradecane (15) exhibit considerable selectivity to alkali cations. In the latter system, relative permeabilities to the alkali cations, expressed as cation transference numbers, were K+ > Cs+ > Na+ > Rb+ > Li' (15). And, the self-diffusion rates of Na+, K+, and Rb+ m, asured separately through phosphatidylserine vesicles (17) foflowed the series K+ > Rb+ > Na+ (1.0:0.82:0.01).…”

Section: Discussionmentioning

confidence: 94%

Characterization of Passive Ion Transport in Plasma Membrane Vesicles of Oat Roots

Sze

Hodges²

1976

Plant Physiol.

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The passive influx and efflux of inorganic ions across plasma membrane vesicles purified from extracts of Avena sativa roots were investigated. Uptake was measured by incubating the vesicles in a radioisotope for various times. The "loaded" vesicles were separated from the external solution by gel filtration. Efflux was measured by dialyzing the preloaded vesicles.Ion transport was differentiated from superficial ion binding by (a) the time course of association of radioisotope with the vesicles; (b) the rate of loss of radioisotope from the vesicles; (c) the linear increase in isotope associated with the vesicles as the external concentration was increased; (d) the enhanced loss of radioisotope from the vesicles induced by Triton X-100; and (e) the low amount of isotope associated with the vesicles at low temperatures.The plasma membrane vesicles were differentially permeable to the alkali cations with the order of decreasing permeation being K+ > Rb+ > Cs' > Na+ > Li'. The relative transport of Rb+, Na+, and Cl-across the plasma membrane vesicles was about 1.0:0.50:0.18. The permeability coefficient (P) for Rb+ was estimated to be 0.29 ± 0.15 x 10-8 cm/sec. ATP (and ADP) decreased the passive uptake of Rb+ into the vesicles, however, this effect did not appear to be related to the ATPase of the plasma membrane.Elucidation of the mechanism of ion transport across the plasma membrane and tonoplast of plant cells is complicated by the simultaneous transport of ions across both of these membranes as well as by the confounding influences of other aspects of metabolism. Fcr these reasons, it would be desirable to investigate the transport of ions across a single type of membrane vesicle. This can be accomplished only by using isolated membrane vesicles. Such studies have been conducted with plasma membrane vesicles isolated from bacteria (11) and animal cells (2, 3), but similar studies have not been reported for specific plant membranes. However, there are reports concerned with ion fluxes in a mixture of membrane vesicles obtained from Lemna (21) and cotyledons of Phaseolus (12). The latter study was of particular interest sl. ce it was found that ATP had an effect on the K+ content of the vesicles. However, it was not shown whether the effect of ATP was specific and whether it involved a physical effect or an energy transduction.We have previously reported on the purification of plasma membrane vesicles from oat roots (9, 10), and in this paper we present data concerning the passive fluxes of ions into and out of these vesicles.

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Some electrical properties of lipid bilayer membranes

Cited by 56 publications

References 23 publications

Phosphorylation without protonmotive force

Phosphorylation without protonmotive force

Properties of lipid bilayer membranes separating two aqueous phases: The effects of Fe+3 on electrical properties

Characterization of Passive Ion Transport in Plasma Membrane Vesicles of Oat Roots

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