Some effects of propane-1,2-diol on human platelets

Arnaud, Françoise; Hunt, Charles J.; Pegg, D.E.

doi:10.1016/0011-2240(90)90003-m

Cited by 15 publications

(7 citation statements)

References 21 publications

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“…Propylene glycol has also been successfully used for the preservation of RBCs 20 and of mammalian embryos 21 although its toxicity has been reported to rise rapidly above 2 mol per L 22 . Arnaud and Pegg 23 have investigated the osmotic response of PLTs to both glycerol and propylene glycol.…”

Section: Cryoprotectantsmentioning

confidence: 99%

Cryopreservation of living cells: principles and practice

2007

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Increasingly, the cryopreservation of living cells is being attempted by researchers whose primary interest and experience is with the medical applications of those cells or tissues and whose prior experience with cryobiology may be negligible. It is therefore generally necessary to imitate some regimen used by others, perhaps with some other cell type and attempt to optimize the recovery empirically. This article makes no attempt to provide specific protocols for the many individual cell types. Rather it is a primer that may help to give such investigators an insight into the basic principles of cell freezing, cryoprotectants, and, particularly, their addition and removal. Finally, the article summarizes the five different approaches to applied cryopreservation: ultrarapid freezing and thawing, controlled-rate freezing, freezing with nonpenetrating polymers, vitrification, and equilibrium freezing.

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Section: Cryoprotectantsmentioning

confidence: 99%

Cryopreservation of living cells: principles and practice

2007

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“…observation). 300 x 106 cells/ml was chosen as it is recommended for other assays [1,23]. The mixing between the F and D populations was done at the time of assay and the cell concentration was measured again after mixing.…”

Section: Calibrationmentioning

confidence: 99%

“…Transmittance of the PC was read on a spectrophotometer (Lambda 4A, Perkin-Elmer) at the wavelength of 610 nm [17,23]. The measurements were read at room temperature (21°C) against the autologous plasma of the unit.…”

Section: Assaysmentioning

confidence: 99%

“…All the assays were performed after the platelet samples were incubated at 37°C for 30 min [5,23,24], Cell Count. Platelets were counted by means of an electronic cell Counter device (System 9000 Hematol ogy analyzer, Scrono-Bakcr).…”

Section: Assaysmentioning

confidence: 99%

“…The maximal aggregation (Tm) was recorded 10 min after the addition of the ADP. The percent of aggregation response was computed as [23]:…”

Section: Assaysmentioning

confidence: 99%

See 2 more Smart Citations

Measurement of Transmitted Light as an Indicator of Cryopreserved Platelet Viability

Arnaud¹,

Goodrich²

1996

Pathophysiol Haemos Thromb

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This study was designed to establish an alternative technique to the morphology scoring method described by Kunicki et al. [Transfusion 1975;15:414-421] using light transmission. A reference scale for platelet quality (expressed as a percentage of fresh cells) was established with a mixture of fresh platelets and damaged platelets frozen without cryoprotectant. The transmittance, read against autologous plasma, was compared to morphology, hypotonic stress response (HSR) and ADP-induced aggregation. The results showed that transmittance, morphology and HSR were related linearly to the percentage of fresh platelets. There was a significant correlation between relative transmittance (RT) and morphology (r = 0.932), and RT and HSR (r = 0.91), but a very poor correlation was found between transmittance and aggregation. Measurements of transmittance provided an estimation of the global aspect of a population of cryopreserved platelets in terms of shape, size and composition, and the relative transmittance of a sample to that of the fresh population could predict morphology and HSR. It is concluded that these measurements offer a valid alternative method to the subjective morphology scoring obtained with cryopreserved platelets.

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Frozen/Thawed Platelets: Importance of Osmotic Tolerance and Platelet Subpopulations

Arnaud¹

1999

Cryobiology

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Some effects of propane-1,2-diol on human platelets

Cited by 15 publications

References 21 publications

Cryopreservation of living cells: principles and practice

Cryopreservation of living cells: principles and practice

Measurement of Transmitted Light as an Indicator of Cryopreserved Platelet Viability

Frozen/Thawed Platelets: Importance of Osmotic Tolerance and Platelet Subpopulations

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