Some Effects of Maleic Hydrazide on Internode Elongation, Cell Enlargement, and Stem Anatomy

Greulach, Victor A.; Haesloop, John G.

doi:10.1002/j.1537-2197.1954.tb14303.x

Cited by 18 publications

(9 citation statements)

References 13 publications

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“…Previous studies have indicated that much of the inhibitory action of MH is due to its effect on mitosis (6,10). There are, however, several lines of evidence in addition to those presented in this study, which indicate that this supposition is not entirely correct.…”

Section: Discussioncontrasting

confidence: 73%

Maleic hydrazide-auxin interractions in water uptake of potato discs

Taylorson

Holm

1961

Plant Physiol.

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Section: Discussioncontrasting

confidence: 73%

Maleic hydrazide-auxin interractions in water uptake of potato discs

Taylorson

Holm

1961

Plant Physiol.

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“…Many previous investigations have led to a commonly accepted conclusion that MH is an antimitotic agent (see ref 4 and refs cited therein). Our finding that MH inhibits cell division in dormant lettuce seed contributes new information in two respects.…”

Section: Discussionmentioning

confidence: 99%

Action of Maleic Hydrazide on Dormancy, Cell Division, and Cell Expansion

Haber

White

1960

Plant Physiol.

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Maleic hydrazide (MH) is of practical and theoretical interest as a growth-regulating chemical that can inhibit plant growth without causing obvious morphological abnormalities (13,14). After recent reports of growth interactions between MH and gibberellic acid (GA) (2, 10), Brian and Hemming called attention to the many growth effects for which MH has actions opposite to those of GA (1). From studies of interactions of MH and GA on pea stem extension, these authors concluded that "MH prevents the response to GA of GA-sensitive plants" and that it is, therefore, likely that "MH inhibits growth by blocking some essential reaction at a stage preceding that where GA normally exerts its effect" (1). In these experiments, we have studied the effects of MH and compared them with those of GA on three systems: lettuce seed germination, mitosis in the absence of growth by expansion in dormant lettuce seeds (5, 7), and growth by cell expansion in the absence of mitosis in y-irradiated wheat (6). The results show that there is no specific interaction between MH and GA either on lettuce seed germination or on the growth of wheat seedlings, and MH seems to exert direct effects on cell division but not on cell expansion during seedling growth [which is in contrast to previously studied GA effects on these same systems (6. 7)]. Similarities between effects of MH and of ionizing radiation are noted. MATERIALS AND METHODSExperiments were performed with lettuce (Lactuca sativa L., var. Big Boston White) and wheat (Triticumz vulgare Vill., (Triticum aestivum L.), var. Thorne). Big Boston White was used because its germination is more sensitive to MH than any of several other varieties we tested. We sowed 100 mg of lettuce seed on one piece of Whatman No. 1 filter paper moistened with 3.5 ml of solution at pH 5.7 in a covered 9 cm Petri dish. The dishes within closed copper sterilization cans (and thus in continuous darkness) were placed in a constant-temperature water bath. The criterion for germination was visual detection of radicle protrusion after 2 days at 260 C. excised, fixed, and examined for mitotic figures as previously described (5, 7). Some wheat grains were given 800 kr of -y rays at 49 kr/hr from a Co60 source described elsewhere (3). The wheat was from the same batch used in previous studies (6) and was grown under the same controlled conditions for 12 days (with light intensity of approximately 450 ft-c during the 16-hour day). Growth was measured as length of the first leaf 12 days after sowing, at which time growth of the irradiated plants growing without mitosis was complete (6). RESULTSIf the suggestion of Brian and Hemming (1), that MH prevents response to GA, is generally true for gibberellin responses then we should expect GA to be less effective than other germination stimulators in relieving the dormancy imposed by MH. Accordingly, we studied the effects of germination stimulators (GA, kinetin, thiourea) in combination with germination inhibitors (MH, coumarin, 2,4-dinitrophenol, mannitol, and nicotine) on...

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“…Keys to deficiency symptoms with various elements known to be essential to the growth of higher plants have been published (1,2,5,6,8). These have been of significant general service to physiologists.…”

Section: Literature Citedmentioning

confidence: 99%

“…Naylor and Davis (2) and Isenberg et al (3,4) suggest that maleic hydrazide is a respiratory inhibitor, at least at higher concentrations. Greulach and coworkers (5,6) report that maleic hydrazide restricts growth primarily by inhibiting mitosis. Leopold and Klein (7) suggest that it is an antiauxin.…”

mentioning

confidence: 99%