A method for the biosynthesis of f5N-labelled S-adenosylmethionine from 15NH4+ and L-methionine by yeast is reported. Culture conditions suitable f o r economy in the utilization of the isotope were developed, and the use of y-irradiated yeast (1 Mrad) for this purpose was explored. The irradiated yeast proved as effective as non-irradiated yeast, but it did not offer any advantage. 15N-Adenine was obtained from S-adenosylmethionine by hydrolysis; its isolation by ion exchange chromatography is described.