Some effects of glucose and calcium upon the metabolism of kidney slices from adult and newborn rats

Robinson, J. R.

doi:10.1042/bj0450068

Cited by 188 publications

(31 citation statements)

References 8 publications

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“…Spermatozoa were flushed, at 34°C, from the cauda epididymides (Hutton et al 1980) with the phosphate-buffered saline (PBS) devised by Robinson (1949) but with glucose omitted. The spermatozoal suspension (40-60 ml) was centrifuged at 2500 g for 10 min at 20°C.…”

Section: Collection Of Spermatozoamentioning

confidence: 99%

Inhibition of Fructolytic Enzymes in Boar Spermatozoa by (S)-a-Chlorohydrin and l-Chloro-3-hydroxypropanone

RJones¹,

Bubb²,

Murdoch³

et al. 1986

Aust. Jnl. Of Bio. Sci.

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When boar spermatozoa were incubated with the (S)-isomer of the male antifertility agent a-chlorohydrin the activity of glyceraldehyde-3-phosphate dehydrogenase was inhibited. The (R)-isomer had no significant effect on the activity of this enzyme whereas (R,S)-3-chlorolactaldehyde caused an inhibition of its activity and also in that of lactate dehydrogenase. The in vitro production of (S)-3-chlorolactaldehyde, the active metabolite of (S)-a-chlorohydrin, was attempted by incubating boar spermatozoa with l-chloro-3-hydroxypropanone. Preliminary results lead us to propose that this compound is converted into (S)-3-chlorolactaldehyde as well as to another metabolite which is an inhibitor of other enzymes within the fructolytic pathway.

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Section: Collection Of Spermatozoamentioning

confidence: 99%

Inhibition of Fructolytic Enzymes in Boar Spermatozoa by (S)-a-Chlorohydrin and l-Chloro-3-hydroxypropanone

RJones¹,

Bubb²,

Murdoch³

et al. 1986

Aust. Jnl. Of Bio. Sci.

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“…Two X 10' freshly isolated CML cells in 0.2 ml of 5% glucose were added to 0.3 ml of autologous heparinized plasma (500 U of heparin per 25 ml blood), 1.2 ml of Robinson's salt mixture (10), pH 7.4, supplemented with 100 mg glucose per 100 ml and 209 mg sodium bicarbonate per 100 ml, and 0.2 ml of the leukocyte homogenate being tested or 0.1 M phosphate buffer as a control. After incubation for 1 hour at 370 C in a screw-top vial, 1 ,uc of H'TdR (SA, 0.36 c per mmole)4 in 0.1 ml of isotonic saline was added and the incubation continued for an hour.…”

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confidence: 99%

Thymidine Catabolism by Normal and Leukemic Human Leukocytes*

Marsh¹,

Perry²

1964

J. Clin. Invest.

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“…The balanced saline medium (Robinson, 1956) was essentially the same as the medium 'A' (Robinson, 1949) in which slices of rat kidney were shown to maintain a constant oxygen uptake for 4 hr. A stock solution was prepared by mixing the following reagents: 0-770 m-KCI, 8 ml.…”

Section: Methodsmentioning

confidence: 99%

Effects of water content upon the respiration and the cations of kidney slices

Little¹,

Robinson²

1967

The Journal of Physiology

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SUMMARY1. Cortical slices from the kidneys of adult rats were incubated aerobically at 38.50 C in Warburg manometers in an ordinary balanced saline medium and also in 'low-ion' media consisting of 20 mm Tris buffer to which 0-30 g polyethylene glycol ('PEG 6000')/100 ml. was added to control the swelling of the tissue.2. Oxygen uptake in the low ion media containing less than 2 m-equiv/l. of Na+ and K+ was maximal when the water content of the slices was kept normal by 14 % PEG 6000 in the medium, and was about two thirds of the oxygen consumption in a balanced saline medium.3. Slices respiring in the low-ion media retained potassium in concentrations 10-20 times the concentration in the medium; the ratio of tissue to external concentration of K+ was inversely related to the water content of the tissue. 4. Ratios of K+/Na+ concentration in tissue water were maximal and equal to 10-25 when water content was near normal and the ratio Na+/K+ in the medium was between 1.5 and 2-0.5. Cryoscopic measurements showed that 14 % of PEG 6000 contributed 140 m-osmole/kg to the osmolality of the low-ion media in which slices maintained a normal water content. 6. It was not possible to interpret the volume of the cells as determined by the balance between the osmotic effect of PEG 6000 in the medium and the colloid osmotic pressure of intracellular proteins.

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Some effects of glucose and calcium upon the metabolism of kidney slices from adult and newborn rats

Cited by 188 publications

References 8 publications

Inhibition of Fructolytic Enzymes in Boar Spermatozoa by (S)-a-Chlorohydrin and l-Chloro-3-hydroxypropanone

Inhibition of Fructolytic Enzymes in Boar Spermatozoa by (S)-a-Chlorohydrin and l-Chloro-3-hydroxypropanone

Thymidine Catabolism by Normal and Leukemic Human Leukocytes*

Effects of water content upon the respiration and the cations of kidney slices

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