Some effects of denervation upon white and red muscles in carp

Wittenberger, C.; Coprean, D

doi:10.1016/0300-9629(77)90243-2

Cited by 4 publications

(4 citation statements)

References 16 publications

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“…In general, these studies have shown a greater independence of muscle properties toward neural control in comparison to muscles of nonpiscine vertebrates. For example, the denervation of muscle fibers of the carp by spinal cord crush resulted in small changes in metabolic markers and contractile protein content (Wittenberger and Coprean, 1977). In the zebrafish (Brachydanio rerio) a similar lesion brought about changes in fiber C SA after 2 weeks, but not in all muscle fiber types (van Asselt et al, 1990).…”

Section: Resistance Of Muscle Phenotype To Change After Neural Input mentioning

confidence: 99%

Reexpression of Myogenic Proteins in Mature Electric Organ after Removal of Neural Input

Unguez

Zakon

1998

J. Neurosci.

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The electric organ (EO) of the weakly electric fish Sternopygus macrurus derives from striated myofibers that fuse and suppress many muscle properties. Mature electrocytes are larger than muscle fibers, do not contain sarcomeres, or express myosin heavy chain (MHC) or tropomyosin. Furthermore, electrocytes express keratin, a protein not expressed in muscle. In S. macrurus the EO is driven continuously at frequencies higher than those of the intermittently active skeletal muscle. The extent to which differences in EO and muscle phenotype are accounted for by activity patterns, or innervation per se, was determined by assessing the expression of MHC, tropomyosin, and keratin 2 and 5 weeks after the elimination of (1) activity patterns by spinal transection or (2) all synaptic input by denervation.Immunohistochemical analyses showed no changes in muscle fiber phenotypes after either experimental treatment. In contrast, the keratin-positive electrocytes revealed an upregulation of MHC and tropomyosin. Nearly one-third of all electrocytes expressed MHC (35%) and tropomyosin (25%) 2 weeks after spinal transection, whereas approximately two-thirds (61%) expressed MHC 2 weeks after denervation. After 5 weeks of denervation or spinal transection, all electrocytes contained MHC and tropomyosin. Newly formed sarcomere clusters also were observed in denervated electrocytes. The MHC expressed in electrocytes corresponded to that present in a select population of muscle fibers, i.e., type II fibers. Thus, the elimination of electrical activity or all synaptic input resulted in a partial reversal of the electrocyte phenotype to an earlier developmental stage of its myogenic lineage.

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Section: Resistance Of Muscle Phenotype To Change After Neural Input mentioning

confidence: 99%

Reexpression of Myogenic Proteins in Mature Electric Organ after Removal of Neural Input

Unguez

Zakon

1998

J. Neurosci.

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“…The change of the metabolic pattern of the chicken pectoral from the red type in the young animal to the white type in the adult fowl takes place through some "critical" periods which we have described in a previous paper (28). O n the other hand, it is well known that the effects of denervation depend on the muscle type (2, 5 , 27). For the above reasons, a muscle like the chicken's pectoral deserves, in our opinion, more attention than is generally paid to it.…”

Section: Introductionmentioning

confidence: 84%

Metabolic Modifications in the Denervated Pectoral Muscle of the Chicken

Wittenberger¹,

Giurgea²,

Coprean³

1981

Zentralblatt für Veterinärmedizin Reihe A

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Summary Denervated pectoral muscles of chickens exhibited decreased contents of glycogen and total proteins, increased content of RNA, lowered ratio of myofibrillar to sarcoplasmic protein fractions, and lowered activities of myofibrillar ATPase and of total glycogen phosphorylase activities, compared with the values for the heterolateral control muscles. The amplitude of the modifications was dependent both on the age at which denervation was performed and on the time that elapsed after operation. Partial return to normal of the parameters occurred gradually, especially in young chickens; no return to normal was found in birds operated by the „critical” age of 12–14 days. Zusammenfassung Metabolische Veränderungen im denervierten Brustmuskel von Küken Die Denervation des Brustmuskels bei Küken führte zu folgenden Veränderungen: Abnahme des Glykogen‐ und Proteingehaltes, Zunahme der RNA, Verminderung des Verhältnisses zwischen myofibrillären und sarkoplasmatischen Proteinfraktionen, sowie erniedrigte Aktivitäten der myofibrillären ATPase und der Gesamt‐Glykogen‐Phosphorylase im Vergleich zu den Werten für die heterolateralen Kontrollmuskeln. Das Ausmaß der Veränderungen war sowohl abhängig vom Alter, in welchem die Denervation durchgeführt wurde, als auch vom zeitlichen Abstand zur Operation. Teilweise wurde eine allmähliche Rückkehr zu den Normalwerten beobachtet, speziell bei jungen Küken. Normalwerte wurden jedoch nicht mehr erreicht, wenn die Operation im „kritischen” Alter von 12 bis 14 Tagen erfolgte. Résumé Modifications métaboliques dans le muscle pectoral dénervé de poussins La dénervation du muscle pectoral chez des poussins a provoqué les modifications suivantes: diminution du taux de glycogène et de protéines, augmentation de l'ARN, diminution du rapport entre les fractions protéiques myofibrillaires et sarcoplasmiques, un abaissement des activités de l'ATPase myofibrillaire et de la glycogène‐phosphorylase totale par comparaison avec les valeurs pour les muscles hétérolatéraux de contrôle. l'étendue des modifications dépendait aussi bien de l'age au moment de la dénervation, que de la période à partir de l'opération. On a partiellement observé un retour graduel aux valeurs normales chez les jeunes poussins en particulier. Les valeurs normales n'ont cependant jamais été atteintes lorsque l'opération a eu lieu à l'âge «critique» de 12 à 14 jours. Resumen Las modificaciones metabólicas en el músculo pectoral denervado del pollito La denervación del músculo pectoral conducía en los pollitos a las modificaciones siguientes: disminución del contenido en glucógeno y proteína, aumento del ARN, disminución de la relación entre las fracciones proteicas mio‐fibrilares y sarcoplásmicas, actividades minoradas de la ATFasa miofibrilar y de la glucógeno‐fosforilasa total en comparación con los valores de los músculos testigos heterolaterales. La magnitud de las modificaciones dependía tanto de la edad en la cual se ejecutó la denervación como del espacio de tiempo transcurrido después de la operación. Algunas veces...

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“…Few studies have examined the role of the nervous system on muscle properties in adult teleosts. Studies in piscine species report small changes in metabolic and contractile protein content in carp (69) and few changes in MHC fiber type conversion in zebrafish (2,45) after a 14-day denervation period. In the common carp (Cyprinus carpio), a 2 wk denervation period resulted in small changes in metabolic markers and contractile protein content (69).…”

Section: A Shout-out For Comparative Studiesmentioning

confidence: 96%

“…Studies in piscine species report small changes in metabolic and contractile protein content in carp (69) and few changes in MHC fiber type conversion in zebrafish (2,45) after a 14-day denervation period. In the common carp (Cyprinus carpio), a 2 wk denervation period resulted in small changes in metabolic markers and contractile protein content (69). A similar 2 wk denervation of adult zebrafish (Brachydanio rerio) muscle led to few changes in fiber type conversion, but not in all muscle fiber types (2,45).…”

Section: A Shout-out For Comparative Studiesmentioning

confidence: 96%

Properties of skeletal muscle in the teleost Sternopygus macrurus are unaffected by short-term electrical inactivity

Güth

Chaidez

Samanta³

et al. 2016

Physiological Genomics

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Skeletal muscle is distinguished from other tissues on the basis of its shape, biochemistry, and physiological function. Based on mammalian studies, fiber size, fiber types, and gene expression profiles are regulated, in part, by the electrical activity exerted by the nervous system. To address whether similar adaptations to changes in electrical activity in skeletal muscle occur in teleosts, we studied these phenotypic properties of ventral muscle in the electric fish Sternopygus macrurus following 2 and 5 days of electrical inactivation by spinal transection. Our data show that morphological and biochemical properties of skeletal muscle remained largely unchanged after these treatments. Specifically, the distribution of type I and type II muscle fibers and the cross-sectional areas of these fiber types observed in control fish remained unaltered after each spinal transection survival period. This response to electrical inactivation was generally reflected at the transcript level in real-time PCR and RNA-seq data by showing little effect on the transcript levels of genes associated with muscle fiber type differentiation and plasticity, the sarcomere complex, and pathways implicated in the regulation of muscle fiber size. Data from this first study characterizing the acute influence of neural activity on muscle mass and sarcomere gene expression in a teleost are discussed in the context of comparative studies in mammalian model systems and vertebrate species from different lineages.

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Some effects of denervation upon white and red muscles in carp

Cited by 4 publications

References 16 publications

Reexpression of Myogenic Proteins in Mature Electric Organ after Removal of Neural Input

Reexpression of Myogenic Proteins in Mature Electric Organ after Removal of Neural Input

Metabolic Modifications in the Denervated Pectoral Muscle of the Chicken

Properties of skeletal muscle in the teleost Sternopygus macrurus are unaffected by short-term electrical inactivity

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