Some Effects of Adrenaline and Anti-Adrenaline Compounds on Platelets in vitro and in vivo

O'Brien, J.R.

doi:10.1038/200763a0

Cited by 296 publications

(119 citation statements)

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“…ADP -induced platelet aggregability was determined by the method of O' Brien (11). Erythrocytic adenosine deaminase activity was estimated by modified method of Giuseppe Giusti described by Severini (12).…”

Section: I) Hematological Parametersmentioning

confidence: 99%

Studies on biochemical changes with special reference to oxidant and antioxidants in malaria patients

Kulkarni

Suryakar

Sardeshmukh

et al. 2003

Indian J Clin Biochem

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Oxidative stress plays an important role in the development of maladal anemia. The present study was undertaken to study the role of oxidant and antioxidants in the patients of Plasmodium falciparum malada (n = 25), Plasmodium vivax malada (n = 25) as against the normal control subjects (n =25). The parameters included are the hematological [hemoglobin, erythrocyte adenosine deaminase (ADA) activity, ADP -induced platelet aggregation] and serum total lipid peroxide as an index of oxidative stress and antioxidants [erythrocytic superoxide dismutase (SOD) activity, serum vitamin E] & serum iron.Significant alterations in all above parameters were noted in both groups of malaria patients as compared to control subjects. Maximum significant alterations in hematological parameters were noticed in P. falciparum infection as compared to P. vivax malada (p<0.001). Substantial dse in serum total lipid peroxides and a significant reduction in antioxidants such as serum vitamin E and serum iron were noted in R falciparum malaria as compared to R vivax malada (p< 0.001), whereas maximum decline in erythrocytic SOD activity was observed in R vivax infection as compared to R falciparum malaria (p<0.05). Follow-up examination revealed the restoration of the levels of all biochemical parameters to the normal level after 20 days of antimalarial therapy.The study specified severity of P. falciparum malaria and also functional duality of oxidant.

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Section: I) Hematological Parametersmentioning

confidence: 99%

Studies on biochemical changes with special reference to oxidant and antioxidants in malaria patients

Kulkarni

Suryakar

Sardeshmukh

et al. 2003

Indian J Clin Biochem

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“…10 -11 However, direct platelet activation by Epi is usually seen at supraphysiological concentrations (micromolar range) and experimentally only under certain conditions. 1243 Lower, more physiological concentrations of Epi can sensitize platelets to other agonists, such as ADP, in vitro, 12 ' 14 and Epi infusions causing high physiological levels of Epi in plasma also seem to activate platelets in vivo.…”

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confidence: 99%

Epinephrine sensitizes human platelets in vivo and in vitro as studied by fibrinogen binding and P-selectin expression.

Hjemdahl

Chronos

Wilson

et al. 1994

Arterioscler Thromb

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Epinephrine (Epi) infusion influences platelet activation markers in vivo, but in vitro studies have mainly examined supraphysiologica] Epi concentrations and have yielded conflicting results. In this study whole-blood flowcytometric measurements of platelet fibrinogen binding and P-selectin expression were used to compare enhancement of ADP (0.1 to 10 /irnol/L)-induced platelet activation by Epi infusion in vivo (0.1 and 0.4 nmol • kg" 1 • min" 1 ) and by Epi in vitro (10 and 50 nmol/L) in nine healthy volunteers. ADP caused concentration-dependent increases in the percentage of platelets that bound fibrinogen (from 4.4 ±0.9% to 69.9±4.2%) and that expressed P-selectin (from 4.5±0.5% to 44.2±3.8%). Fibrinogen and P-selectin binding indices (FgBI and PSBI; calculated from mean fluorescence intensity and percentage of positive cells) also increased from 0.18 ±0.03 to 11.70±1.99 for FgBI and from 0.22±0.03 to 2.34±0.29 for PSBI. Epi concentration-dependently enhanced fibrinogen binding and P-selectin expression in vitro (by »»30% at the midportion of the ADP curve at 10 nmol/L Epi; P<.001 for both by ANOVAs). High-dose Epi infusion enhanced FgBI similarly and increased maximal P-selectin expression by 38%. Epi (50 nmol/L in vitro) enhanced platelet activation further, whether samples were taken with or without prior Epi infusion. Total expression of gfycoprotein Ilb/IIIa was unaffected by Epi infusion, but glycoprotein Ib expression per platelet was reduced (/*<.05). These in vivo and in vitro effects of Epi on platelet responses to agonist stimulation indicate a prothrombotic potential for sympathoadrenal activation in humans.

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“…Norepinephrine has extensively been described as a trigger for the induction of human platelet aggregation [35][36][37][38]. Von Känel et al [39] also showed that acute mental stress, during which NE levels are significantly increased, increases thrombin activity and fibrin turnover reliant on b2-adrenergic receptor functioning and associated catecholamine activity [39].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Sibutramine, a Serotonin-Norepinephrine Reuptake Inhibitor, on Platelets and Fibrin Networks of Male Sprague-Dawley Rats: A Descriptive Study

Schoor¹,

Oberholzer²,

Bester³

et al. 2014

Ultrastructural Pathology

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Sibutramine is used in the treatment of obesity due to its ability to influence feelings of hunger and satiety by inhibiting the re-uptake of serotonin and noradrenalin in the central nervous system (CNS). Sibutramine use has been associated with numerous adverse events in particular cardiovascular complications possibly due to the formation of thrombi. This ultrastructural descriptive study investigated the effect of sibutramine on blood coagulation, specifically the effect on morphology of platelets and fibrin networks using scanning electron microscopy. Male Sprague-Dawley rats treated with either a recommended therapeutic dose [low dosage 1.32 mg/kg] or a toxicological higher dose [high dosage 13.2 mg/kg] of sibutramine for 28 days were used and compared to control animals. Blood samples were collected and plasma smears were prepared for platelet evaluation. Following the addition of thrombin to the plasma samples, the morphology of the fibrin clots was evaluated. Platelet evaluation by scanning electron microscopy revealed morphology typical of a prothrombotic state with a characteristic excessive platelet activation in both low-dose (LD) and high-dose (HD) rats. The fibrin clots of sibutramine-treated rats, LD and HD revealed fused thick fibers with thin fibers forming a netlike structure over the thick fibers which differ considerably from the organized structure of the control animals. It can be concluded that sibutramine alters the ultrastructure of platelets and fibrin networks creating a prothrombotic state.

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Some Effects of Adrenaline and Anti-Adrenaline Compounds on Platelets in vitro and in vivo

Cited by 296 publications

References 5 publications

Studies on biochemical changes with special reference to oxidant and antioxidants in malaria patients

Studies on biochemical changes with special reference to oxidant and antioxidants in malaria patients

Epinephrine sensitizes human platelets in vivo and in vitro as studied by fibrinogen binding and P-selectin expression.

The Effect of Sibutramine, a Serotonin-Norepinephrine Reuptake Inhibitor, on Platelets and Fibrin Networks of Male Sprague-Dawley Rats: A Descriptive Study

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