“…Recently, work has begun to focus on the membrane-bound enzyme (4,5). Steady-state kinetic information shows little functional difference between the two enzyme preparations (6,7). However, positive cooperativity in substrate (acetylthiocholine) concentration-dependent steady-state velocity has been observed with a membranebound AcChoEase fragment (5); corresponding cooperative inhibition by an active site inhibitor (procaine) was noted in the same study.…”