Some Developmental and Molecular Aspects of Somatic Embryogenesis (Nonzygotic Embryogenesis)

Mordhorst, A. P.; Charbit, Erika; Vries, S.C. de

doi:10.1201/9780203506561.ch15

Cited by 3 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Species that easily form somatic embryos such as Arabidopsis, carrot (Daucus carota), white spruce (Picea glauca), alfalfa (Medicago sativa), and soybean (Glycine max) have been used for the identification and characterization of genes and proteins that are important for the induction and maintenance of embryonic development (Mordhorst et al, 1997;Perry et al, 1999;Ikeda-Iwai et al, 2002;Yazawa et al, 2004;Mordhorst et al, 2005;Quiroz-Figueroa et al, 2006;Raghavan, 2006). For example, the SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) gene was isolated on the basis of its expression in embryogenic cells of carrot suspension cultures (Schmidt et al, 1997).…”

mentioning

confidence: 99%

“…The Poa pratensis ortholog of the SERK1 gene, PpSERK, and a START domain-containing gene, APOSTART, recently have been implicated in apomictic development in P. pratensis based on expression analyses of these genes in nucellar and megaspore mother cells of apomictic and sexual varieties (Albertini et al, 2005). As well, somatic embryo models have been useful for studying cell differentiation processes in plants and for increasing our understanding of the functional aspects of genes already implicated in embryogenesis (Mordhorst et al, 2005;Quiroz-Figueroa et al, 2006).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Transcript Profiling and Identification of Molecular Markers for Early Microspore Embryogenesis inBrassica napus

et al. 2007

View full text Add to dashboard Cite

Isolated microspores of Brassica napus are developmentally programmed to form gametes; however, microspores can be reprogrammed through stress treatments to undergo appropriate divisions and form embryos. We are interested in the identification and isolation of factors and genes associated with the induction and establishment of embryogenesis in isolated microspores. Standard and normalized cDNA libraries, as well as subtractive cDNA libraries, were constructed from freshly isolated microspores (0 h) and microspores cultured for 3, 5, or 7 d under embryogenesis-inducing conditions. Library comparison tools were used to identify shifts in metabolism across this time course. Detailed expressed sequence tag analyses of 3 and 5 d cultures indicate that most sequences are related to pollen-specific genes. However, semiquantitative and real-time reverse transcription-polymerase chain reaction analyses at the initial stages of embryo induction also reveal expression of embryogenesis-related genes such as BABYBOOM1, LEAFY COTYLEDON1 (LEC1), and LEC2 as early as 2 to 3 d of microspore culture. Sequencing results suggest that embryogenesis is clearly established in a subset of the microspores by 7 d of culture and that this time point is optimal for isolation of embryo-specific expressed sequence tags such as ABSCISIC ACID INSENSITIVE3, ATS1, LEC1, LEC2, and FUSCA3. Following extensive polymerase chain reaction-based expression profiling, 16 genes were identified as unequivocal molecular markers for microspore embryogenesis in B. napus. These molecular marker genes also show expression during zygotic embryogenesis, underscoring the common developmental pathways that function in zygotic and gametic embryogenesis. The quantitative expression values of several of these molecular marker genes are shown to be predictive of embryogenic potential in B. napus cultivars (e

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%