Some common signal transduction events are not necessary for the elicitor-induced accumulation of silymarin in cell cultures of Silybum marianum

Sánchez-Sampedro, María Angeles; Fernández‐Tárrago, Jorge; Corchete, Purificación

doi:10.1016/j.jplph.2007.12.009

Cited by 9 publications

(4 citation statements)

References 35 publications

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“…In contrast, 30 min contact with an elicitor was sufficient for alkaloid formation in Escholztia californica cultures 24 h later and the authors suggest that a short pulse activated early events enough to trigger alkaloid biosynthesis. As reported by Sánchez-Sampedro et al (2008), some common and rapid signal transduction events, like calcium fluxes, phosphorylation/dephosphorylation cascades or H 2 O 2 generation, are not necessary for the elicitor-induced accumulation of silymarin in cell cultures of S. marianum. However, upon elicitation, a metabolic reprogramming that affected amino acid and carbohydrate metabolism was observed in the system.…”

Section: Elicitor Contact Timementioning

confidence: 75%

Elicitation of silymarin in cell cultures of Silybum marianum: effect of subculture and repeated addition of methyl jasmonate

2009

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Production of silymarin and the effect of the elicitor, methyl jasmonate (MeJA), was monitored in cell cultures of Silybum marianum over 4 years. Silymarin concentrations gradually declined after prolonged subculture, making the success of elicitor strategy limited in long-term cultures. The continuous presence of MeJA in cultures for an extended period was necessary for induction of silymarin accumulation. A repeated elicitor strategy was not a good option for improving silymarin productivity in batch cultures. Removal of medium from elicited cultures and addition of fresh medium avoided the toxic effects of elicitor accumulation, allowing the system to respond to a repeated MeJA treatment without loss of productivity.

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Section: Elicitor Contact Timementioning

confidence: 75%

Elicitation of silymarin in cell cultures of Silybum marianum: effect of subculture and repeated addition of methyl jasmonate

2009

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“…rosmarinic acid in Coleus blumei (Szabo et al, 1999), or hypericin in Hypericum perforatum (Walker et al, 2002). Methyl jasmonate (MeJA), alone or in combination with yeast extract (YE), strongly promote the accumulation of silymarin (Elwekeel et al, 2012a;Elwekeel et al, 2012b;Sánchez-Sampedro et al, 2008). Treatment of cell suspension culture of S.marianum with YE elicitor improved production and release of silymarin into the culture medium to a level of about 3-fold higher than that of the control (Sánchez-Sampedro et al, 2005a).…”

Section: Introductionmentioning

confidence: 99%

Proteome Analysis of Milk Thistle (Silybum Marianum L.) Cell Suspension Cultures in Response to Methyl Jasmonate and Yeast Extract Elicitors

Pourjabar¹,

Azimi²,

Mostafaie³

et al. 2019

Appl. Ecol. Env. Res.

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Elicitors cause biosynthesis and accumulation of secondary metabolites by inducing defense responses. In this study, we treated the cell suspension cultures of Silybum marianum L. with MeJA (methyl jasmonate) (100 μM) and YE (yeast extract) (0.1 w/v) as elicitors and measured the content of Silymarin accumulation by HPLC (High Performance Liquid Chromatography). Accumulation of Silymarin significantly increased after 48 h of MeJA and YE application. In order to investigate the effect of abiotic (MeJA) and biotic (YE) stresses on expression of proteins in S. marianum cell suspension cultures, we employed high resolution two-dimensional gel electrophoresis coupled with MALDI-TOF-TOF (matrix assisted laser desorption ionization time of flightetime of flight) mass spectrometry. At least, 249 protein spots showed reproducible and significant changes in the gel. Spots were up or down regulated upon MeJA and YE treatments. Ten protein spots were identified using MALDI-TOF-TOF-MS. The identified proteins belong to different functional categories. The proteins were classified based on carbohydrate metabolism (spots 6 and 22), Nitrogen metabolism (spot 20), storage protein (spot 29), transport process (spot 12), protein modification and chaperones (spot 8), pathogenesis related (spot 1 and 2) and secondary metabolism (spot 3 and 53). The potential role of these proteins in the biosynthetic pathway of flavonoids and the finding of proteins in non-sequenced plants such as S.marianum requires further research.

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“…These results suggest that inhibition of external and internal calcium fluxes play a significant role in flavonolignans metabolism in S. marianum cell cultures. Sánchez-Sampedro et al (2005b) reported that yeast extract and methyl jasmonate elicited the production of silymarin. Elicitation is one of the most effective approaches to enhance the yield of secondary metabolites in in vitro cultures (Namdeo, 2007).…”

Section: Cell Culturementioning

confidence: 99%

Silymarin, Natural Flavonolignans from Milk Thistle

AbouZid¹

2012

Phytochemicals - A Global Perspective of Their Role in Nutrition and Health

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Some common signal transduction events are not necessary for the elicitor-induced accumulation of silymarin in cell cultures of Silybum marianum

Cited by 9 publications

References 35 publications

Elicitation of silymarin in cell cultures of Silybum marianum: effect of subculture and repeated addition of methyl jasmonate

Elicitation of silymarin in cell cultures of Silybum marianum: effect of subculture and repeated addition of methyl jasmonate

Proteome Analysis of Milk Thistle (Silybum Marianum L.) Cell Suspension Cultures in Response to Methyl Jasmonate and Yeast Extract Elicitors

Silymarin, Natural Flavonolignans from Milk Thistle

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