Some characters of the pollen of spring and summer flowering common oak (Quercus robur L.)

Batos, Branislava; Bobinac, Martin

doi:10.2298/abs1201085b

Cited by 3 publications

(3 citation statements)

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“…This indicates that the pollen could be routinely dried and preserved at low temperatures with relatively simple methods, akin to those used for conventional seed banking. For example, Q. coccinea and Q. alba pollen survived for at least a year when dried at 25-35% RH and stored at 2 • C [46,48], germinability of dry pollen of Q. robur was preserved up to two years at −20 • C [49], dry pollen of Q. petraea and Q. robur was stored for at least 1 year at −18 • C and used in breeding programmes for acorn production [50], and freeze-dried pollen retained some viability after 300 days at −5 • C [46]. The pollen of some Quercus sp.…”

Section: Pollenmentioning

confidence: 99%

“…The levels of nutrients, sucrose and agar influence pollen germination and need to be determined and adjusted per species. In the case of Quercus sp., pollen germination media usually contains 10-20% sucrose and is often solidified with 0.75-1% agar ( [48,49,51,56] for Q. coccinea, Q. petraea and Q. robur). Sometimes mineral nutrients have been added to the basic sucrose solutions, such as 20 ppm boron for Q. alba, Q. coccínea, and Q. ilicifolia [46] or MS salts for Q. robur [56].…”

Section: Pollenmentioning

confidence: 99%

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The Cryobiotechnology of Oaks: An Integration of Approaches for the Long-Term Ex Situ Conservation of Quercus Species

Ballesteros

Pritchard

2020

Forests

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Conventional dry seed storage is unlikely for about one third of all tree species (and nearly half of evergreen rain forest trees) as they probably produce desiccation sensitive (recalcitrant) seeds. Consequently, international ex situ conservation targets for threatened trees will be difficult to achieve without innovation, especially in cryobiotechnology. We assessed progress in the development of various cryobiotechnology approaches for the preservation of oaks (Quercus), which are keystone species of functioning landscapes, important to the bioeconomy and under increasing threats from the spread of pests and diseases under a changing climate. Various tissues of oaks can be used for banking, from pollen grains to embryo axes. Pollen from five oak species have been shown to be highly desiccation tolerant, making dry pollen storage at low temperatures (including in liquid nitrogen) a valuable technology to support conservation and breeding programs. Somatic embryo (SE) technology and/or shoot tip in vitro technology is available for 39 species, and SE cryopreservation is routinely performed on three commercial species and shoot tips cryopreservation successful in two more species. Seed embryonic axes are the preferred explants for oak ex situ conservation, with tissue survival and regeneration of plants after cryopreservation recorded for 14 and seven species respectively; although differential responses between the shoot and root meristems in the axes are known. Dormant bud preservation seems promising, but is under-researched. Overall, these results indicate the possibility of establishing an integrated platform for the ex situ conservation of oak species based on cryobiotechnology. Challenges of explant choice, optimization of methodologies and large-scale application do remain. However, multiple approaches for the cryopreservation of oak genetic resources are available and implementation programmes should not be delayed, particularly in the centres of species diversity.

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Section: Pollenmentioning

confidence: 99%

Section: Pollenmentioning

confidence: 99%

The Cryobiotechnology of Oaks: An Integration of Approaches for the Long-Term Ex Situ Conservation of Quercus Species

Ballesteros

Pritchard

2020

Forests

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“…The samples with less than 5% of germinated grains were treated as samples with no germination. Measurements were done using a Leica Galen III light microscope (magnification x100) [36,37], with a camera (CCD Camera Topica TP/5001).…”

Section: Experimental Procedures and Determination Of Pollen Charactermentioning

confidence: 99%

Pollen viability in Quercus robur L.

Batos

2017

Arch biol sci (Beogr)

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The variability of viability (germination rate and the length of pollen tubes) of fresh pedunculate oak (Quercus robur L.) pollen grains was studied in vitro on a medium containing 15% sucrose. Spatial variability was studied by sampling fresh pollen grains from a total of thirteen trees at four different sites in the area of Belgrade (Kosutnjak, Banovo Brdo, Ada Ciganlija and Bojcin Forest) in a single year (2011). In order to assess temporal variability and determine the effects of climate change on a small time scale, we studied the viability of the pollen grains collected from one tree at the Banovo Brdo site in six different years (2004, 2005, 2006, 2007, 2011 and 2012). Interindividual variability was tested on the pollen grains sampled from eight trees at Ada Ciganlija in 2004. The percentage values of the pollen grain germination rate and the pollen tube length showed no statistically significant differences between the sites. However, the studied characteristics of the pollen grain viability (germination rate and pollen tube length) showed statistically significant differences in both temporal (between the pollen collection years) and interindividual variability. This type of research makes a valuable contribution to pedunculate oak breeding programs through the identification of trees with stable production and a good quality of pollen. Furthermore, it can be important in defining the patterns of spatial, temporal and individual variability of pollen grain viability under the influence of climate factors, which are showing compelling changing trends from year to year.

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Pollen Cryopreservation of Coniferous Serbian Spruce (Picea omorika/Panč./Purkyne) and Deciduous Pedunculate Oak (Quercus robur L.) Species

Batos

2023

Springer Protocols Handbooks

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Some characters of the pollen of spring and summer flowering common oak (Quercus robur L.)

Cited by 3 publications

References 18 publications

The Cryobiotechnology of Oaks: An Integration of Approaches for the Long-Term Ex Situ Conservation of Quercus Species

The Cryobiotechnology of Oaks: An Integration of Approaches for the Long-Term Ex Situ Conservation of Quercus Species

Pollen viability in Quercus robur L.

Pollen Cryopreservation of Coniferous Serbian Spruce (Picea omorika/Panč./Purkyne) and Deciduous Pedunculate Oak (Quercus robur L.) Species

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