Some Characteristics of Mitochondria Extracted from Higher Plants

Bonner, Walter D.; Voss, Dinor O.

doi:10.1038/191682a0

Cited by 43 publications

(14 citation statements)

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“…Preliminary experiments indicated that tom,a)to fruiit mitochondria were stable for at least 1 day after isolation anid showed a delayed response to the first addition of ADP whiile oxidizing succinate. Suich a dellayed responnse to the first addition of ADP with succinate as suibstratte h!as been reported for mitoch,ondria from seve,ral plant species and tissues (2,6,9,13,14). Wiskich and Bonner (14) attributed this deliay to inihibitory amounts of oxa,loacetate, wrhile Verleur ( 13) attributed it to the quality of the mitochondria.…”

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confidence: 79%

Properties of Succinate Oxidation in Tomato Fruit Mitochondria

1968

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The stability of isolated yeast mritochondria during refrigerated storage has been sttudied (9), but the comparable stability of m)iltochondria from a higher plant has not been reported. Preliminary experiments indicated that tom,a)to fruiit mitochondria were stable for at least 1 day after isolation anid showed a delayed response to the first addition of ADP whiile oxidizing succinate. Suich a dellayed responnse to the first addition of ADP with succinate as suibstratte h!as been reported for mitoch,ondria from seve,ral plant species and tissues (2, 6,9,13,14). Wiskich and Bonner (14) attributed this deliay to inihibitory amounts of oxa,loacetate, wrhile Verleur ( 13) attributed it to the quality of the mitochondria. In view of the conflicting interpretations of this delay, and th,e stability of tomato fru,iit mitocjhondria, a study of sucecinate oxidationi in these mitochondria was condtuted. This paper reporits effects dtue to storage and some of the factors controll1ing the delayed respon.se ito the first addition of ADP. Materials and MethodsTomaito fruit (Lycopersi(cmn (sclienton7 AiMill.) were picked in the greenhouse or field the day of use. Mature green fruit were used except for table I which also includes data from tomato fruit vine-ripened in the greenhouse 5 d'ays past incipient coloring. The entire mitochonndrial isolation was done at 00 to 40. A sample of 62.5 g of outer perlicarp was rinised, chilled in crtushed ice, and then

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confidence: 79%

Properties of Succinate Oxidation in Tomato Fruit Mitochondria

1968

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“…Plant Sto proteins have di-iron centres in their active sites (Bonner et al, 1986;Minagawa et al, 1990;Moore et al, 1995a;Moore et al, 1995b). This separates them completely from the bacterial alternative terminal oxidases, which use heme in their active sites (Gennis and Stewart, 1996).…”

Section: Introductionmentioning

confidence: 99%

SHAM‐sensitive alternative respiration in the xylose‐metabolizing yeast Pichia stipitis

Shi

Cruz

Sherman

et al. 2002

Yeast

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SHAM-sensitive (STO) alternative respiration is present in the xylose-metabolizing, Crabtree-negative yeast, Pichia stipitis, but its pathway components and physiological roles during xylose metabolism are poorly understood. We cloned PsSTO1, which encodes the SHAM-sensitive terminal oxidase (PsSto1p), by genome walking from wild-type CBS 6054 and subsequently deleted PsSTO1 by targeted gene disruption. The resulting sto1-deletion mutant, FPL-Shi31, did not contain other isoforms of Sto protein that were detectable by Western blot analysis using an alternative oxidase monoclonal antibody raised against the Sto protein from Sauromatum guttatum. Levels of cytochromes b, c, c 1 and a·a 3 did not change in the sto1-mutant, which indicated that deleting PsSto1p did not alter the cytochrome pool. Interestingly, the sto1-deletion mutant stopped growing earlier than the parent and produced 20% more ethanol from xylose. Heterologous expression of PsSTO1 in Saccharomyces cerevisiae increased its total oxygen consumption rate and imparted cyanide-resistant oxygen uptake but did not enable growth on ethanol, indicating that PsSto1p is not coupled to ATP synthesis. We present evidence that the mitochondrial NADH dehydrogenase complex (Complex I) was present in wild-type CBS 6054 but was bypassed in the cells during xylose metabolism. Unexpectedly, deleting PsSto1p led to the use of Complex I in the mutant cells when xylose was the carbon source. We propose that the non-proton-translocating NAD(P)H dehydrogenases are linked to PsSto1p in xylose-metabolizing cells and that this non-ATP-generating route serves a regulatory function in the complex redox network of P. stipitis. Published in

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“…Respiratory control has been demonstrated with mitochondria from both animals [4,5] and plants [2], and its equivalent in photosynthesis (photosynthetic control) has been observed in isolated pea chloroplasts [3]. Respiratory control has not however been demonstrated with preparations from bacteria, although increased rates of oxygen uptake by the inclusion of phosphate or phosphate-accepter have been reported [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%