Some causes of inter-laboratory variation in the results of comet assay

Сирота, Н. П.; Жанатаев, А. К.; Kuznetsova, Elena; Khizhnyak, Eugenii P.; Anisina, Elena A.; Дурнев, А. Д.

doi:10.1016/j.mrgentox.2014.05.003

Cited by 25 publications

(26 citation statements)

References 23 publications

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“… Sirota et al. (2014) studied inter-laboratory variation of comet assay factors, like slide brands, duration of alkali treatment and electrophoresis conditions, and they found that laboratory differences were associated with electrophoresis conditions, especially the temperature during alkaline electrophoresis, which affects the rate of conversion of alkali labile sites to single stranded breaks ( Sirota et al. , 2014 ).…”

Section: Ionizing Radiation-associated Dna Damage Radiotherapy and Mmentioning

confidence: 99%

Ionizing radiation-induced DNA injury and damage detection in patients with breast cancer

2015

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Breast cancer is the most common malignancy in women. Radiotherapy is frequently used in patients with breast cancer, but some patients may be more susceptible to ionizing radiation, and increased exposure to radiation sources may be associated to radiation adverse events. This susceptibility may be related to deficiencies in DNA repair mechanisms that are activated after cell-radiation, which causes DNA damage, particularly DNA double strand breaks. Some of these genetic susceptibilities in DNA-repair mechanisms are implicated in the etiology of hereditary breast/ovarian cancer (pathologic mutations in the BRCA 1 and 2 genes), but other less penetrant variants in genes involved in sporadic breast cancer have been described. These same genetic susceptibilities may be involved in negative radiotherapeutic outcomes. For these reasons, it is necessary to implement methods for detecting patients who are susceptible to radiotherapy-related adverse events. This review discusses mechanisms of DNA damage and repair, genes related to these functions, and the diagnosis methods designed and under research for detection of breast cancer patients with increased radiosensitivity.

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Section: Ionizing Radiation-associated Dna Damage Radiotherapy and Mmentioning

confidence: 99%

Ionizing radiation-induced DNA injury and damage detection in patients with breast cancer

2015

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“…Briefly, images were opened in CometScore software and the individual cells were scored using the scoring tool which automatically measures the % tail DNA and olive tail moment for that particular cell. Approximately, 100 cells per gel were analysed [28,44,48].…”

Section: Alkaline Comet Assaymentioning

confidence: 99%

Differential molecular and cellular responses of GLP-1 secreting L-cells and pancreatic alpha cells to glucotoxicity and lipotoxicity

Vasu

Moffett

McClenaghan

et al. 2015

Experimental Cell Research

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“…After the classification, HiComet could identify around 90% of non-overlapped cells in one comet assay image. In order to evaluate DNA damages correctly, we calculated the heterogeneity of response [ 24 – 26 ] (e.g., the distribution of % DNA in the tail). When the true distribution of % DNA in the tail is normal, the discarded 10% cells would cause only 1.7% decrease in the confidence level with the same confidence interval (e.g., 95% confidence interval using 50 comets = 93.7% confidence interval using 41 comets).…”

Section: Resultsmentioning

confidence: 99%

HiComet: a high-throughput comet analysis tool for large-scale DNA damage assessment

Lee

Sim³

et al. 2018

BMC Bioinformatics

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BackgroundDNA damage causes aging, cancer, and other serious diseases. The comet assay can detect multiple types of DNA lesions with high sensitivity, and it has been widely applied. Although comet assay platforms have improved the limited throughput and reproducibility of traditional assays in recent times, analyzing large quantities of comet data often requires a tremendous human effort. To overcome this challenge, we proposed HiComet, a computational tool that can rapidly recognize and characterize a large number of comets, using little user intervention.ResultsWe tested HiComet with real data from 35 high-throughput comet assay experiments, with over 700 comets in total. The proposed method provided unprecedented levels of performance as an automated comet recognition tool in terms of robustness (measured by precision and recall) and throughput.ConclusionsHiComet is an automated tool for high-throughput comet-assay analysis and could significantly facilitate characterization of individual comets by accelerating its most rate-limiting step. An online implementation of HiComet is freely available at https://github.com/taehoonlee/HiComet/.

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Some causes of inter-laboratory variation in the results of comet assay

Cited by 25 publications

References 23 publications

Ionizing radiation-induced DNA injury and damage detection in patients with breast cancer

Ionizing radiation-induced DNA injury and damage detection in patients with breast cancer

Differential molecular and cellular responses of GLP-1 secreting L-cells and pancreatic alpha cells to glucotoxicity and lipotoxicity

HiComet: a high-throughput comet analysis tool for large-scale DNA damage assessment

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