Some biological reactions of the organism after exposure to nanosecond repetitive pulsed microwaves

Kereya, A V; Жаркова, Л. П.; Bolshakov, M. A.; Кутенков, О. П.; Rostov, V. V.

doi:10.1088/1742-6596/1115/2/022015

Cited by 1 publication

(1 citation statement)

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“…A growing body of literature has recognised the non-thermal effect of pulsed microwave on bacterial systems (Rougier et al, 2014). It has been shown that pulsed microwave directly interacts with specific (polar) molecules, thus causing a variety of bioeffects, which further leads to failure of the intracellular oxidative defence machinery and DNA damage-mediated inactivation of bacterial cells (Kereya et al, 2018;Shaw et al, 2021). High power pulse microwave (HPPM) has been developed as an innovative non-thermal technology which offers many advantages over other processing technology.…”

Section: Introductionmentioning

confidence: 99%

Sterilising effect of high power pulse microwave on Listeria monocytogenes

Zhang¹,

Wang²,

Wu³

et al. 2022

IFRJ

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In the present work, Listeria monocytogenes was used as the target strain to investigate the sterilising potential and mechanism of high power pulse microwave (HPPM). Results showed that the inactivation was positively correlated with the pulse frequencies and operating times. The count of Listeria monocytogenes was decreased by 5.09 log CFU/mL under 200 Hz for 9 min, which was used as the optimised condition to further explore the sterilisation mechanism. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images showed that the L. monocytogenes cells of untreated group presented intact surfaces, clear boundary, and its intracellular contents distributed uniformly in the cytoplasm. Following treatment, the cell wall surfaces began to deform in small areas, and cell membranes were severely ruptured, thus resulting in the appearance of electron transmission areas. Extracellular protein and nucleic acid contents, represented by OD260 nm and OD280 nm, increased with the increase in operating time significantly. After treatment, SDS-PAGE profiles of whole-cell proteins displayed that the protein bands became lighter or even disappeared. Na+ K+-ATPase activities and intracellular ATP content decreased by 72.97 and 79.09%, respectively. This was consistent with the cell viability of L. monocytogenes observed by confocal laser scanning microscopy. Overall, the sterilisation mechanism of HPPM on L. monocytogenes may be caused by membrane damage, intracellular component leakage, and energy metabolism hindrance.

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Section: Introductionmentioning

confidence: 99%