Some biochemical responses of buffalo PMN cells to various stimuli

Sarmah, Satya Ranjan; More, T.

doi:10.1016/0147-9571(95)00017-8

Cited by 4 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Buffaloes are also susceptible to bacterial endotoxaemic conditions. Activatioq of buffalo PMN cells with ConA and LPS caused release of MPO ( Table 1) and has been reported earlier also (Sarmah and More, 1996). MPO is associated with primary granules of neutrophils (Cooray, et al, 1993) and is an important component of antimicrobial system of neutrophils.…”

Section: Resultsmentioning

confidence: 83%

Effect of Neutrophil Activation on Proteinase Inhibitor, Myeloperoxidase and Superoxide Production in Buffaloes

Reddy

Kumar

2003

Journal of Applied Animal Research

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 83%

Effect of Neutrophil Activation on Proteinase Inhibitor, Myeloperoxidase and Superoxide Production in Buffaloes

Reddy

Kumar

2003

Journal of Applied Animal Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…LPS or OZ stimulate immune cells, following exposure to infection. Our previous observations suggested that buffalo PMN cells synthesise proteins and peptides similar to those of other species (13,22,23). Steinbeck et al (24) also reported that protein synthesis was necessary in bovine PMN cells for an antibody-dependent cytotoxicity response.…”

Section: Discussionmentioning

confidence: 81%

“…Superoxide (O 2-) and H 2 O 2 production were assessed in the presence and absence of ConA, digitonin and SNP (all from Sigma, St Louis, MO, USA). The optimum levels of the anti-inflammatory agents, cortisol and indomethacin (both from Gibco BRL, Mumbai, India), and of LPS, OZ, ConA, digitonin and SNP, used in this study, were based on our earlier studies on buffalo PMN cells (8,13,14). The level of VpHCl (Sigma) used in the ATPase activity studies, was based on reports from studies in other species (15).…”

Section: Activation Of Pmn Cellsmentioning

confidence: 99%

In VitroEffects of Certain Membrane-acting Agents on Superoxide and Hydrogen Peroxide Production, Protein Synthesis and Membrane ATPase Activity in Buffalo PMN Cells

Das

Reddy

et al. 2007

Altern Lab Anim

View full text Add to dashboard Cite

Polymorphonuclear (PMN) cells play a key role in innate immunity, due to their ability to produce reactive oxidants such as superoxide (O2–) and hydrogen peroxide (H2O2), and to release antimicrobial proteins and peptides stored in their lysosomal granules. In the present study, the effects of the activation of buffalo PMN cells with various membrane-acting agents were evaluated in terms of O2– and H2O2 production, the activities of membrane ATPases, and protein synthesis. Studies involving the incorporation of 35S-methionine revealed significant protein-synthesising ability in resting PMN cells and in cells treated with lipopolysaccharide (LPS), as well as with opsonised zymosan (OZ). Protein synthesis, as judged by fluorography of the cytosolic fraction, showed more than 12 bands, whilst the cytoskeletal fraction showed 2–3 bands. PMN activation with concanavalin A (ConA), digitonin and sodium nitroprusside (SNP) resulted in increased O2– and H2O2 production. However, in the presence of anti-inflammatory agents such as indomethacin and cortisol, the production of O2– and H2O2 by these cells was found to decline. Studies pertaining to membrane ATPases revealed that verapamil hydrochloride (VpHCl) significantly increased total ATPase and Na+K+ATPase activity. ConA treatment yielded only a moderate level of activity. Similarly, digitonin up to 24μM also caused a significant increase in ATPase activity. Our observations indicate that these membrane-acting agents influenced oxygen-dependent and oxygen-independent microbicidal mechanisms in buffalo PMN cells.

show abstract

Immunology of the Buffalo

1998

Handbook of Vertebrate Immunology

View full text Add to dashboard Cite

Some biochemical responses of buffalo PMN cells to various stimuli

Cited by 4 publications

References 22 publications

Effect of Neutrophil Activation on Proteinase Inhibitor, Myeloperoxidase and Superoxide Production in Buffaloes

Effect of Neutrophil Activation on Proteinase Inhibitor, Myeloperoxidase and Superoxide Production in Buffaloes

In VitroEffects of Certain Membrane-acting Agents on Superoxide and Hydrogen Peroxide Production, Protein Synthesis and Membrane ATPase Activity in Buffalo PMN Cells

Immunology of the Buffalo

Contact Info

Product

Resources

About