Some Biochemical Changes and Role of Micro RNA 34 a-5p and Micro RNA 365a-5p as A Diagnostic Marker in Chronic Hepatitis C (HCV).

Abstract

This study aimed to investigate Micro RNA 34a-5p and Micro RNA 365a-5p in addition to the traditional biochemical analysis as a useful diagnostic biochemical marker for chronic Hepatitis C Virus (HCV). The present study included 17 individuals with chronic liver disease, who admitted to Benha University Hospital. After investigating 5 patients from all patients, were found to be positive polymerase chain reaction (PCR) chronic HCV. 10 control negative serum samples were collected from the control healthy group. Biochemical analysis was done for investigating liver function parameters alanine transaminase (ALT), aspartate transaminase (AST), total bilirubin (TBil), direct bilirubin (DBil), gama glutamyl transferase (GGT), alkaline phosphatase (ALP) and albumin (Alb) and hematological analysis including total leucocytic count (TLC), hemoglobuin concentration (Hb) and platelet count (PLT), Alpha feto protein (AFP) as a tumor marker indicator. RNA was extracted from serum samples for gene expression of microRNA34 a-5p and microRNA 365a-5p. HCV group showed a significant increase in liver function parameters (ALT, AST, TBIL, DBIL, GGT, ALP) while there was a significant reduction in the albumin levels. Moreover, there was a significant decrease in TLC with non-significant decrease in the HB concentration and the platelet count. AFP concentration in HCV group was significantly increased compared to the control group. HCV group revealed a significant increase (8.98 ± 0.34) in miRNA34, with a significant decrease in miRNA365 (0.25 ± 0.03). We concluded that miRNA34a-5p and miRNA365a-5p expressions in addition to different biochemical analysis can be used as a useful diagnostic marker for HCV diagnosis.