Some (bacilli) like it hot: genomics of <scp><i>G</i></scp><i>eobacillus</i> species

Studholme, David J.

doi:10.1111/1751-7915.12161

Cited by 40 publications

(44 citation statements)

References 72 publications

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“…In the media where every salt was tested separately, the best results were achieved with KCl, MgCl 2 or MnSO 4 , the activity in supernatant during the growth was increased up to 160 AU/ml. The same results were observed in the medium that was supplemented with all 8 salts or in After testing all salts and combinations of it in this work, the highest bacteriocin activity was achieved in media supplemented combining KCl and MgCl 2 or CaCl 2 and ZnSO 4 or MnSO 4 and NaHCO 3 . In this case the highest activity during the growth in these modified media was increased up to 320 AU/ml.…”

Section: Growth Medium Optimization For Higher Cell Growth and Bactersupporting

confidence: 82%

“…Evaluation of bacteriocin activity in media supplemented with every salt separately shows that activity may be enhanced using KCl, MgCl 2 or MnSO 4 . The best results can be achieved combining two salts: KCl and MgCl 2 , CaCl 2 and ZnSO 4 or MnSO 4 and NaHCO 3 .…”

Section: Discussionmentioning

confidence: 99%

“…are attractive to the biotechnology industry as source of thermostable enzymes and natural products digesters of lignocellulose, bioremediators of hydrocarbons or as platforms for biofuel production [2,3]. Some Geobacillus spp.…”

Section: Introductionmentioning

confidence: 99%

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Medium Optimization for Bacteriocin Production and Bacterial Cell Growth of Geobacillus sp. 15 Strain

Kaunietis¹,

Pranckutė²,

Lastauskienė³

et al. 2017

J Antimicrob Agents

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In this study we determined that Geobacillus sp. 15 strain secretes antibacterial compound-bacteriocin. Only few bacteriocin producing thermophilic bacteria of this genus have been identified to the date. Also, we optimized medium composition for the better cell growth of this strain and increased the yield of secreted bacteriocin. In this work we showed that some particular salts or their combinations may have impact on higher growth of Geobacillus sp. 15 strain bacteria or their produced bacteriocins. These results can facilitate research on this strain and its secreted bacteriocin. These antibacterial proteins and peptides are promising natural agents as an alternative to antibiotics in medicine or veterinary and to traditional preservatives in food industry. Optimized composition of the growth medium can be very useful for studies of other Geobacillus spp. strains and their produced bacteriocins. Furthermore, these data may be used to increase the biomass of Geobacillus bacteria and the yield of protein when it is dependent on bacterial cell yield.

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Section: Growth Medium Optimization For Higher Cell Growth and Bactersupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

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Medium Optimization for Bacteriocin Production and Bacterial Cell Growth of Geobacillus sp. 15 Strain

Kaunietis¹,

Pranckutė²,

Lastauskienė³

et al. 2017

J Antimicrob Agents

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“…After the isolation of NUB36 from soil, it was assigned to Bacillus stearothermophilus (Chen et al, 1986). Genetic analysis of this strain showed that it is too distinct phylogenetically to belong to the species G. stearothermophilus (Studholme et al, 1999) and should be reclassified as Geobacillus caldoxylosilyticus or assigned to a novel species of Geobacillus (Studholme, 2015;Zeigler, 2005). However, this strain still holds the original name allocated upon discovery.…”

Section: Hosts: Most Commonly Used Geobacillus Strains For Transformamentioning

confidence: 99%

“…could be modified and adapted for Geobacillus spp. Moreover, the increasing number of publicly available complete genome sequences of geobacilli (Studholme, 2015) provides more information for a better understanding of their biology and the development of host-vector systems.…”

Section: Introductionmentioning

confidence: 99%

Genetic engineering of Geobacillus spp.

Kananavičiūtė

Čitavičius

2015

Journal of Microbiological Methods

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Dimerization of Proline Dehydrogenase from Thermus thermophilus Is Crucial for Its Thermostability

Huijbers

Westphal

et al. 2019

Biotechnology Journal

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Thermus thermophilus proline dehydrogenase (TtProDH) catalyzes the first step in proline catabolism. The thermostable flavoenzyme consists of a distorted triosephosphate isomerase (TIM) barrel and three N-terminal helices: αA, αB, and αC. Using maltose-binding protein (MBP) fused constructs, it has been recently demonstrated that helix αC is crucial for TtProDH catalysis and for tetramerization through positioning of helix α8. Here, the structural features that determine the thermostability of TtProDH are reported. Selective disruption of two ion pairs in the dimerization interface of several MBP-TtProDH variants result in the formation of monomers. The newly created monomers have improved catalytic properties but their melting temperatures are decreased by more than 20°C. Sequence comparison suggests that one of the ion-pairs involved in dimerization is unique for ProDHs from Thermus species. In summary, intermolecular ion-pairs improve the thermostability of TtProDH and a trade-off is made between thermostability and catalytic activity.

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Some (bacilli) like it hot: genomics of Geobacillus species

Cited by 40 publications

References 72 publications

Medium Optimization for Bacteriocin Production and Bacterial Cell Growth of Geobacillus sp. 15 Strain

Medium Optimization for Bacteriocin Production and Bacterial Cell Growth of Geobacillus sp. 15 Strain

Genetic engineering of Geobacillus spp.

Dimerization of Proline Dehydrogenase from Thermus thermophilus Is Crucial for Its Thermostability

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