Some aspects of the use of transglutaminase

Z.S., Z.S. Zobkova; Fursova, T.P.

doi:10.31515/1019-8946-2020-04-42-45

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2022

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“…Многие вещества растений способствуют повышению защитных и компенсаторных реакций организма, оказывают бактерицидное, противовоспалительное, ранозаживляющее, седативное, спазмолитическое и противоболевое воздействие, улучшают процессы пищеварения, кроветворения и др. [12][13][14][15][16][17].…”

Section: Introductionunclassified

Development of Milk Dessert Enriched by Functional Vegetable Additives

Владимировна¹,

Олеговна²

2018

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Section: Introductionunclassified

Development of Milk Dessert Enriched by Functional Vegetable Additives

Владимировна¹,

Олеговна²

2018

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Construction, expression, purification, characterization, and structural analysis of microbial transglutaminase variants

Song

Sheng

Zhou

et al. 2022

Biotech and App Biochem

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Microbial transglutaminase (MTG, EC 2.3.2.13) derived from Streptomyces mobaraensis is widely used in the food and pharmaceutical industry because of its ability to synthesize isopeptide bonds between the proteinogenic side chains of glutamine and lysine. The half-life (t 1/2 ) of the activated wild-type enzyme at 60 • C is 2 min. To improve the activity and thermostability of MTG for higher temperature application, three variants (Mut1, Mut2, and Mut3) were obtained by combining key amino acid mutations on the basis of previous research results.The best variant Mut2 with a specific combination of five of seven substitutions (S2P-S23V-Y24N-R215A-H289Y) shows a 10-fold increased half-life at 60 • C (t 1/2 = 27.6 min), and a 2.4-fold increased specific enzyme activity (39.3 U/mg). As measured by circular dichroism, the curve of Mut2 was basically the same as that of MTG-WT. The structural simulation of Mut2 shows that the overall structure is discoid with a crack, but the crack openings are wider than that of MTG-WT. Furthermore, structural analysis of Mut2 showed that there were seven hydrogen bonds and one π-anion interaction between Mut2 and its adjacent amino acids, and the number of hydrogen bonds was one more than that of MTG-WT (six hydrogen bonds).

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Some aspects of the use of transglutaminase

Cited by 2 publications

References 0 publications

Development of Milk Dessert Enriched by Functional Vegetable Additives

Development of Milk Dessert Enriched by Functional Vegetable Additives

Construction, expression, purification, characterization, and structural analysis of microbial transglutaminase variants

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