Some Aspects of the Relationship of Peptide Structures to Activity in Pituitary Hormones

Li, Choh Hao

doi:10.1016/s0083-6729(08)61059-6

Cited by 26 publications

(3 citation statements)

References 70 publications

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“…It is known that glycine is found in all the protein and polypeptide hormones of the anterior hypophysis. Glycine makes up 7.0 per cent, 6.2 per cent, and 7.7 per cent of the amino acid residues of growth hormone, lactogenic hormone, and adrenocorticotrophic hormone, respectively (24,25). One of the thirteen amino acids of alpha-MSH (intermedin) is glycine (25).…”

Section: Administration Of Labeled Amino Acidmentioning

confidence: 99%

“…Glycine makes up 7.0 per cent, 6.2 per cent, and 7.7 per cent of the amino acid residues of growth hormone, lactogenic hormone, and adrenocorticotrophic hormone, respectively (24,25). One of the thirteen amino acids of alpha-MSH (intermedin) is glycine (25). Another means of incorporation of glyeine into proteins is via its ready transformation into serine (26).…”

Section: Administration Of Labeled Amino Acidmentioning

confidence: 99%

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Identification of the Adrenocorticotrophin-Producing Cells in the Rat Hypophysis by Autoradiography

Siperstein

1963

The Journal of Cell Biology

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The relative rates of protein (hormone) synthesis and secretion by the various cell types in the anterior hypophysis of the rat have bcen studied by means of autoradiography. Normal and adrenalectomized male rats wcre injected with tritiated glycine and their hypophyses removed and fixed at 20, 40, and 90 minutes and 15 hours after injection. Autoradiograms of the hypophysial sections were prepared and autoradiographic grains were counted in thc film overlying the cytoplasm of individual cells. With the aid of this method, a unique cell type was identificd in the hypophyscs of adrenalcctomizcd rats. This ccll is morphologically distinct from "gonadcctomy cells," "thyroidectomy cells," and from previously described normal cell types, and is thcrcfore designated as the "adrenalectomy cell." Among the 7 cell types differentiated in this study, the "adrenalectomy cell" had the highest tritium content and, in addition, at the time intcrvals studied this cell had the fastcst rate of appearance and disappcarance of protein tritium. This autoradiographic evidence of rapid protein (or polypeptide) turnover following adrcnalectomy indicates that the "adrenalectomy cell" is the site of adrenocorticotrophin production in the adrcnalectomized rat. Further autoradiographic and cytological evidence is presented which suggests that the "adrenalectomy cells" may be derived from chromophobes, and that a portion of the "large chromophobcs" as defined in this study may be the sitc of adrenocorticotrophin production in the normal rat.

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Section: Administration Of Labeled Amino Acidmentioning

confidence: 99%

Section: Administration Of Labeled Amino Acidmentioning

confidence: 99%

Identification of the Adrenocorticotrophin-Producing Cells in the Rat Hypophysis by Autoradiography

Siperstein

1963

The Journal of Cell Biology

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“…Readings were begun immediately after adding the Schiff reagent, and repeated at 15 minutes and 1 hour. terminal a-amino alcohols occurring in several of the other tropins (12,13). By means of microspectrophotometry (23) the in vitro results are compared with the PAS reaction in histological sections of the normal human pituitary.…”

mentioning

confidence: 99%

SPECTROPHOTOMETRY OF THE PERIODIC ACID-SCHIFF REACTION WITH PITUITARY HORMONES IN VITRO AND IN HISTOLOGICAL SECTIONS

Fand

Thorell

1962

The Journal of Cell Biology

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The spectral light absorption of the in vitro periodic acid-Schiff reactions of 4 purified pituitary hormones is described. The absorption spectra present a maximum between 560 and 565 mµ. The color developed conforms with Beer's law for the ranges of concentration examined. The different hormones exhibit different chromogenicity per unit of biological activity: the color produced by 1 unit of FSH is equivalent to approximately 2 of TSH, 4 of LH, and 30 of ACTH. Microspectrophotometric measurements of the PAS-positive structures in histological sections of the human pituitary give absorption curves with shapes similar to those obtained in vitro, although quantitative differences exist. It is concluded that under the proper experimental conditions microspectra of the pituitary structures might, in the future, prove to give a quantitative measure of aldehyde groups generated from glycoprotein tropins by periodate oxidation.

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Some Aspects of the Relationship of Peptide Structures to Activity in Pituitary Hormones

Cited by 26 publications

References 70 publications

Identification of the Adrenocorticotrophin-Producing Cells in the Rat Hypophysis by Autoradiography

Identification of the Adrenocorticotrophin-Producing Cells in the Rat Hypophysis by Autoradiography

SPECTROPHOTOMETRY OF THE PERIODIC ACID-SCHIFF REACTION WITH PITUITARY HORMONES IN VITRO AND IN HISTOLOGICAL SECTIONS

Structure–Activity Relationships of the Adrenocorticotropins and Melanotropins: The Synthetic Approach

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