Some Aspects of Respiratory Metabolism During Metamorphosis of Normal and DDT-Resistant House Flies, Musca Domestica L

Sacktor, Bertram

doi:10.2307/1538533

Cited by 24 publications

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“…The activity of the cytochrome c oxidase preparations was determined with a Beck-man model DU spectrophotometer according to the technique previously described (Sacktor (1951 a)). To 3.0 ml.…”

Section: Methodsmentioning

confidence: 99%

The Cytochrome C Oxidase of the House Fly, Musca Domestica L

Sacktor

1952

Journal of General Physiology

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1. Insoluble and "soluble" forms of cytochrome c oxidase were prepared from house flies by methods described for mammalian tissue. A 4.5-fold purification was accomplished by preparing the enzyme in insoluble form (as compared with a crude water brei). The oxidase in "soluble" form was purified 20.0-fold. 2. The absorption spectrum of the "soluble" preparation from flies was similar to that from mammalian tissue, and indicated the presence of cytochromes a + a3 and b. In contrast with the corresponding mammalian enzymes these components were not separable by the existing techniques. 3. The Soret peak seen at 440 mµ and the alpha peak at 600 mµ were due. primarily to cytochromes a3 and a, respectively. 4. The oxidase activity of whole house flies was comparable with that of rat abdominal muscle. In both organisms, this activity was inhibited by cyanide to approximately the same extent. 5. The optimum buffer concentration for oxidase activity, as measured spectrophotometrically, was approximately 0.02 to 0.06 M. This is in agreement with results obtained for mammalian enzyme, as measured both manometrically and spectrophotometrically. 6. The present study has demonstrated the following components of the cytochrome system in house flies: b, c, a, and a3.

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Section: Methodsmentioning

confidence: 99%

The Cytochrome C Oxidase of the House Fly, Musca Domestica L

Sacktor

1952

Journal of General Physiology

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“…Respiratory metabolic rate may be related to rate of breakdown of DDT (Sacktor, 1951). Roth & Lindquist (1953) working with Orlando-resistant houseflies, found that topically applied DDT produced less mortality in flies held at 32 ~ C than at 21 ~ They also found that the rate of DDT absorption was in the reverse order, i.e., greater at the higher temperature by a factor of 6,4%.…”

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DIEL CHANGES IN DDT ABSORPTION AND BREAKDOWN RATES AND RESPIRATORY RHYTHM IN THE HOUSEFLY, MUSCA DOMESTICA

Shipp

Otton

1976

Entomologia Exp Applicata

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Absorption rates in three strains of housefly show little variation when DDT is applied at different times during the 24‐hr cycle. Rates of breakdown of DDT to DDE, however, vary significantly at different times of the day with most rapid breakdown at 05.00–05.30 and 15.00 hr, corresponding to diel peaks in oxygen consumption. The pre‐dawn peak breakdown rates occur at the times at which the flies were found, previously, to be most susceptible to DDT, thus this change in susceptibility must have other causes. ZUSAMMENFASSUNG TAGESZEITLICHE SCHWANKUNGEN IN DDT‐ABSORPTIONS‐ UND ABBAURATEN UND IM ATMUNGSRHYTHMUS BEI DER STUBENFLIEGE, MUSCA DOMESTICA Die Geschwindigkeit, mit der DDT von drei Stubenfliegenrassen absorbiert wurde, zeigte nur wenig Verschiedenheit, wenn es zu verschiedenen Zeiten im Verlauf des 24‐h‐Tages appliziert wurde. Die Rate des Abbaus von DDT zu DDE variierte dagegen im Lauf des Tages, sie war am grössten um 5.00 bis 5.30 Uhr und um 15.00 Uhr, entsprechend den Tagesspitzen des Sauerstoff‐Verbrauchs. Das erste Abbaumaximum (vor Tagesanbruch) fiel zusammen mit der Zeit, in der die Fliegen höchste Empfindlichkeit gegenüber DDT zeigten (vgl. vorige Veröffentlichung). Diese tageszeitspezifische Empfindlichkeit Hess sich demnach keineswegs mit einer (geringeren) Abbaugeschwindigkeit in Zusammenhang bringen.

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“…Agrell (1949) found that in the bow fly, Calliphora erythrocephala, malic, citric, and glutamic dehydrogenases all follow a U-shaped activity curve. Sacktor (1951) found that, in the pupa of the house fly, other factors in addition to the cytochrome system may contribute to the total respiration. A part of the pupal respiration is cyanide-insensitive, and there is in addition to cytochrome oxidase, another cyanide-sensitive system, probably involving tyrosinase.…”

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“…He found a straight line relationship between the activity of cytochrome oxidase and the rate of oxygen consumption and observed that approximately 97 per cent of the respiration was abolished by treatment with cyanide. Sacktor (1951) observed that during the pupal stage of the house fly, Musca domestic.a, both the activity of cytochrome oxidase and the rate of oxygen consumption follow the characteristic U-shaped curve. A similar relationship was shown to exist in the pupa of the Japanese beetle, Popilliajaponica, by Ludwig (1953).…”

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Relationship Between the Activity of the Succinoxidase System and the Rate of Oxygen Consumption During the Embryonic Development of the Mealworm, Tenebrio Molitor Linnaeus

Ludwig

Barsa

1955

The Journal of General Physiology

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For many years cytochrome oxidase has been considered a rate-limiting enzyme involved in oxygen consumption. This relationship was postulated by Wolsky (1938) to explain the U-shaped changes in oxygen consumption which occur during the pupal stage of Drosophila melanogaster. It has received support from the work of Boell (1945) for the embryonic development of the salamander, Amblystoma punctalum. He found a straight line relationship between the activity of cytochrome oxidase and the rate of oxygen consumption and observed that approximately 97 per cent of the respiration was abolished by treatment with cyanide. Sacktor (1951) observed that during the pupal stage of the house fly, Musca domestic.a, both the activity of cytochrome oxidase and the rate of oxygen consumption follow the characteristic U-shaped curve. A similar relationship was shown to exist in the pupa of the Japanese beetle, Popilliajaponica, by Ludwig (1953). However, Wolsky (1941) suggested that the U-shaped respiratory curve in Drosophila pupae may be connected with variations in the activities of the dehydrogenase systems. Agrell (1949) found that in the bow fly, Calliphora erythrocephala, malic, citric, and glutamic dehydrogenases all follow a U-shaped activity curve. Sacktor (1951) found that, in the pupa of the house fly, other factors in addition to the cytochrome system may contribute to the total respiration. A part of the pupal respiration is cyanide-insensitive, and there is in addition to cytochrome oxidase, another cyanide-sensitive system, probably involving tyrosinase.The metabolism of the insect egg may be complicated by the presence of a diapause or resting stage. Bodine (1934) showed that the respiration of the pre-and postdiapause egg of the grasshopper, Melanoplus differentialis, is markedly inhibited by cyanide, whereas that of the diapause egg is cyanideinsensitive. Hence, respiration of the diapanse egg is believed to be mediated by some mechanism other than the cytochrome system, while a portion of the *

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Some Aspects of Respiratory Metabolism During Metamorphosis of Normal and DDT-Resistant House Flies, Musca Domestica L

Cited by 24 publications

References 40 publications

The Cytochrome C Oxidase of the House Fly, Musca Domestica L

The Cytochrome C Oxidase of the House Fly, Musca Domestica L

DIEL CHANGES IN DDT ABSORPTION AND BREAKDOWN RATES AND RESPIRATORY RHYTHM IN THE HOUSEFLY, MUSCA DOMESTICA

Relationship Between the Activity of the Succinoxidase System and the Rate of Oxygen Consumption During the Embryonic Development of the Mealworm, Tenebrio Molitor Linnaeus

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