Some Aspects of Perfluorochemical Emulsion's Interaction with Blood

Abstract

FDC/FTPA (7:3) emulsions stabilized by procsanol (Emulsion 1) and by procsanol with yolk phospholipids (Emulsion 2) were incubated with the donor plasma. After the incubation during 6 hours of Emulsions 1 and 2 with plasma the 36% and 50% decrease of the cholesterol content in plasma was found. Analysis of the lipid content of lipoproteins after the Emulsion 2 administration to rats (2.5 ml/100 g of weight) revealed the 50% decrease of the cholesterol amount in the HDL fraction at 3 and 24 hours posttransfusion. The ratio cholesterol/total phospholipids in the erythrocyte membrane diminished up to 50% as well. The equal degree of the cholesterol adsorption by emulsion from plasma, HDL of rats and erythrocyte membrane is an evidence of nonspecific interaction of PFC particles with the blood components containing cholesterol.

Effect of Perfluorooctyl Bromide on Bacterial Growth

“…A hypothesis may be drawn that these morphological changes in the cell wall observed with electron microscopy are due to an interaction between PFOB and the cell wall of P. aeruginosa. Perfluorochemicals circulating in the bloodstream have been shown to interact with cholesterol-rich lipoproteins (such as high-density lipoproteins) and the erythrocyte membrane, absorbing cholesterol [20,21]. The outer membrane of gram-negative organisms contains lipopolysaccharides with 6-7 covalently linked fatty acid chains [15].…”

Blood monitoring of perfluorocarbon compounds (F-tert-butylcyclohexane, perfluoromethyldecalin and perfluorodecalin) by headspace-gas chromatography-tandem mass spectrometry

“…However, even with these precautions, according to the period of the sport event and especially during long efforts, the PFCs may be totally excreted and undetectable in expired air. Moreover, excretion rates of PFCs are deeply linked to several parameters as their nature, the quantity injected, the size of the emulsion particles [23,24], the emulsifier [25,26], etc. All these variables could lead to important variations for the analysis of PFCs in expired air.…”