Some actions of growth hormone on the perfused cat kidney

A comparison of the direct renal actions of pituitary growth and lactogenic hormones.

“…As previously reported (Lockett & Roberts, 1963) and now confirmed, physiological concentrations of bovine growth hormone (30-70 ,tg/150 ml. blood) reduced the rates of urinary excretion of water, Na and K, but did not change either the renal blood flow (RBF) or the creatinine clearance (GFR).…”

“…The duration of the effect of the human hormone was however less than that ofthe bovine hormone, as has been seen throughout the work. Bovine and ovine growth hormones, as previously reported (Lockett & Roberts, 1963), converted the diuretic effect of aldosterone in blood from headless donors (Davey & Lockett, 1960) into an antidiuretic action like that of aldosterone in blood from intact animals. Figure 4 shows that when human growth hormone, 50 ,ug/150 ml., precedes aldosterone, the latter exerts an antidiuretic, sodium and potassium retaining action in blood from headless animals.…”

“…Eighteen further preparations have been used for examination of the actions of ovine growth and lactogenic hormones and another three for comparison of the effects of bovine growth and lactogenic hormones. The actions of bovine growth hormone as shown by the first twenty-two experiments have been reported (Lockett & Roberts, 1963 hormones were dissolved at a concentration of 1 mg/ml. in 0 9 % NaCl which had been brought to pH 9 0.…”

“…The actions of bovine growth hormone on the isolated kidney have been the subject of a previous paper (Lockett & Roberts, 1963). A more detailed comparison of the direct renal actions of human, bovine and ovine growth hormones, and of the bovine and lactogenic hormones, has now been made since a true similarity between the actions of these large polypeptides would be suggestive of an amino acid sequence common to them all.…”

The formation of peptides with uterine activity from ovine, human and bovine growth hormones and from bovine and ovine lactogenic hormones

“…Oxytocin (Davey & Lockett, 1960) and both growth (Lockett & Roberts, 1963) and lactogenic (Lockett, 1965) hormones can convert this diuretic natriuretic effect of aldosterone into an antidiuresis accompanied by sodium retention in the isolated kidney as can oxytocin and growth hormone in the subtotally hypophysectomized rat (Lees et al 1964). These observations suggested that an oxytocin-like arrangement of amino acids might exist within the molecules of the growth and lactogenic hormones (Lockett, 1965).…”