Somatotopic distribution of corneal afferent neurons in the guinea pig trigeminal ganglion

Keller, Jeffrey T.; Bubel, H. Curt; Wander, Arden H.; Tierney, Bryon E.

doi:10.1016/0304-3940(91)90696-q

Cited by 8 publications

(8 citation statements)

References 12 publications

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“…The presence of mechanical sensitivity in cold neurons was somewhat surprising because responsiveness to mechanical forces is usually absent in cold receptor fibers of the cat or guinea pig (Djouhri and Lawson 2001;Fried et al 2011). However, combined mechanical and cold sensitivity have been reported in sensory fibers of rodents (Burgess and Perl 1967;Keller et al 1991). The proportion of cold neurons was similar to that found in in vitro studies .…”

Section: Low-threshold Mechano-cold Neurons (Ltmr-mc)supporting

confidence: 84%

“…This may be attributable to the closeness of corneal sensory endings to the corneal surface and to the absence of keratinized superficial cells which are found in the skin (Beckers et al 1992;Belmonte and Gallego 1983;Djouhri and Lawson 2001). A large body of data show that afferent innervations of the cornea are either unmyelinated or thinly myelinated (Beckers et al 1992;Hoyes and Barber 1976;Keller et al 1991;Marfurt and Del Toro 1987;Rózsa and Beuerman 1982;Zander and Weddell 1951). Unexpectedly, in our study, we mostly found neurons with A␦ fibers innervating this organ.…”

Section: Corneal Sensory Neuronsmentioning

confidence: 99%

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Relationship between electrophysiological signature and defined sensory modality of trigeminal ganglion neurons in vivo

Boada

2013

Journal of Neurophysiology

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The trigeminal ganglia (TG) innervate a heterogeneous set of highly sensitive and exposed tissues. Weak, innocuous stimuli can evoke pain as a normal response in some areas such as the cornea. This observation implies, however, the capability of low-threshold mechanoreceptors, inducing pain in the normal condition. To clarify this matter, the present study correlates the electrical signature (both fiber conduction velocity and somatic electrical properties) with receptor field, mechanical threshold, and temperature responsiveness of sensory afferents innervating tissues with dissimilar sensitivity (skin vs. cornea) in the trigeminal domain. Intracellular recordings were obtained in vivo from 148 neurons of the left TG of 62 mice. In 111 of these neurons, the peripheral receptor field was successfully localized: 96 of them innervated the hairy skin, while the remaining 15 innervated the cornea. The electrical signature was defined and peripheral responses correlated with tissue target. No high threshold neurons were found in the cornea. Moreover, the electrical signature of corneal afferents resembles nociceptive neurons in the skin. TG skin afferents showed similar membrane electrical signature and sensory modality as skin afferents from dorsal root ganglion, although TG afferents exhibited a shorter duration of afterhyperpolarization then those previously described in dorsal root ganglion. These data suggest than new or different ways to classify and study TG sensory neurons may be required.

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Section: Low-threshold Mechano-cold Neurons (Ltmr-mc)supporting

confidence: 84%

Section: Corneal Sensory Neuronsmentioning

confidence: 99%

Relationship between electrophysiological signature and defined sensory modality of trigeminal ganglion neurons in vivo

Boada

2013

Journal of Neurophysiology

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“…Corneal innervation originates principally in the TG and receives a modest sympathetic innervation from the superior cervical ganglion (Bron et al, 2014;Felipe et al, 1999;Marfurt and Del Toro, 1987;Marfurt et al, 1989). Since the end of the eighties, the localization of a sample population of corneal afferent neurons in rodent TGs has been described using classical histological methods (where the TG is sectioned) and various retrograde tracers such as WGA-HRP, Fluorogold, Fast blue, or CTB (Bron et al, 2014;De Felipe and Belmonte, 1999;Felipe et al, 1999;Ivanusic et al, 2013;Keller et al, 1991;LaVail et al, 1993;Marfurt et al, 1989;Nakamura et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Combined 3DISCO clearing method, retrograde tracer and ultramicroscopy to map corneal neurons in a whole adult mouse trigeminal ganglion

Launay

Godefroy

Khabou

et al. 2015

Experimental Eye Research

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Tissue clearing and subsequent imaging of intact transparent tissues have provided an innovative way to analyze anatomical pathways in the nervous system. In this study, we combined a recent 3-dimensional imaging of solvent cleared organ (3DISCO) procedure, light-sheet microscopy, fluorescent retrograde tracer, and Imaris software to 3D map corneal sensory neurons within a whole adult mouse trigeminal ganglion (TG). We first established the optimized steps to easily and rapidly clear a fixed TG. We found that the 3DISCO procedure gave excellent results and took less than 3 h to clear the TG. In a second set of experiments, a retrograde tracer (cholera toxin B Alexa 594-conjugated) was applied to de-epithelialized cornea to retrograde-labeled corneal sensory neurons. Two days later, TGs were cleared by the 3DISCO method and serial imaging was performed using light-sheet ultramicroscopic technology. High-resolution images of labeled neurons can be easily and rapidly obtained from a 3D reconstructed whole mouse TG. We then provided a 3D reconstruction of corneal afferent neurons and analyzed their precise localization in the TG. Thus, we showed that neurons supplying corneal sensory innervation exhibit a highly specific limited dorsomedial localization within the TG. We report that our combined method offers the possibility to perform manual (on 20 μm sections) and automated (on 3D reconstructed TG) counting of labeled cells in a cleared mouse TG. To conclude, we illustrate that the combination of the 3DISCO clearing method with light-sheet microscopy, retrograde tracer, and automatic counting represents a rapid and reliable method to analyze a subpopulation of neurons within the peripheral and central nervous system.

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“…Within the ophthalmic region, a clear dorsoventral somatotopic arrangement of labeled corneal afferent neurons was established in the guinea pig by Keller et al (1991), with almost no cells in the ventral third of the ganglion. In rabbit, the corneal primary afferent perikarya build up a sharply defined longitudinal column located in the midregion of the ophthalmic area (Marfurt, Kingsley and Echtenkamp, 1989) and in rat, the corneal neurons concentrate markedly in the dorsal part of the ophthalmic region (Marfurt and DelToro, 1987).…”

Section: Centroids For Each Extraocular Muscle Showing Cell Distributmentioning

confidence: 97%

Somatotopic Organization of Primary Afferent Perikarya of the Guinea-pig Extraocular Muscles in the Trigeminal Ganglion: a Post-mortem DiI-tracing Study

Aigner

Lukas

Denk

et al. 2000

Experimental Eye Research

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Somatotopic distribution of corneal afferent neurons in the guinea pig trigeminal ganglion

Cited by 8 publications

References 12 publications

Relationship between electrophysiological signature and defined sensory modality of trigeminal ganglion neurons in vivo

Relationship between electrophysiological signature and defined sensory modality of trigeminal ganglion neurons in vivo

Combined 3DISCO clearing method, retrograde tracer and ultramicroscopy to map corneal neurons in a whole adult mouse trigeminal ganglion

Somatotopic Organization of Primary Afferent Perikarya of the Guinea-pig Extraocular Muscles in the Trigeminal Ganglion: a Post-mortem DiI-tracing Study

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