Somatostatin inhibition of adenylate cyclase activity in different brain areas

Schettini, Gennaro; Florio, Tullio; Meucci, Olimpia; Landolfi, E.; Grimaldi, M.; Ventra, C.; Marino, A

doi:10.1016/0006-8993(89)90889-5

Cited by 70 publications

(77 citation statements)

References 30 publications

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“…A very Ž y4 . high concentration of SS 10 M was required to produce this inhibition although the same concentration was w x w x used by Schettini et al 46 , Bergstrom et al 3 , Garlind eẗ w x w x al. 14 and Nagao et al 35 in their studies on SS-mediated AC activity in rat and human brain.…”

Section: Discussionmentioning

confidence: 96%

“…This finding is consistent with binding studies on post-mortem human and rat brain tissues which have shown that the binding of SS to its recognition site is affected by GTP in a manner consistent with the involvement of a G-protein. In addiw x w x tion, Nagao et al 35 and Shettini et al 46 have shown that SS-reduced cAMP formation in the rat brain occurs via a G-protein coupled to AC. Overall results, plus the Ž .…”

Section: Discussionmentioning

confidence: 99%

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Influence of fluoxetine and p-chloroamphetamine on the somatostatin receptor–adenylyl cyclase system in the rat frontoparietal cortex

Muñoz-Acedo

Izquierdo-Claros

Puebla

et al. 1997

Molecular Brain Research

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There is evidence that suggests a reciprocal functional link between the serotonergic and the somatostatinergic system in the rat Ž . frontoparietal cortex. However, to date, the role of endogenous 5-hydroxytryptamine serotonin on the regulation of the somatostatin Ž .Ž . Ž . SS receptor-adenylyl cyclase AC system remains unclear. In the present study, the administration of fluoxetine 10 mgrkg i.p. , a w 125 x 11 5-hydroxytryptamine uptake inhibitor in a single dose or administered daily for 14 days increased the number of specific I Tyr -SS receptors, with no change in the receptor affinity, in rat frontoparietal cortical membranes. However, the capacity of SS to inhibit Ž . forskolin FK -stimulated AC activity in these membranes was lower than in the control groups. The ability of the stable GTP analogue X Ž Ž . . 5 -guanylylimidodiphosphate Gpp NH p to inhibit FK-stimulated AC activity in frontoparietal cortical membranes was also decreased in Ž . rats acutely and chronically treated with fluoxetine. p-Chloroamphetamine 5 mgrkg i.p. , which leads to a lasting reduction of 5-hydroxytryptamine innervation, administered on days 1, 3 and 5 and the rats sacrificed 1 or 3 weeks after the first injection, decreased the number of SS receptors without changing the receptor affinity. In this experimental group, SS also caused a significantly lower Ž . inhibition of FK-stimulated AC activity. p-Chloroamphetamine had no effect on the ability of Gpp NH p to inhibit FK-stimulated AC activity in frontoparietal cortical membranes at all the time periods studied. The present results suggest that under normal circumstances some SS receptors are under a tonic stimulatory control through the serotonergic system. q 1997 Elsevier Science B.V.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Influence of fluoxetine and p-chloroamphetamine on the somatostatin receptor–adenylyl cyclase system in the rat frontoparietal cortex

Muñoz-Acedo

Izquierdo-Claros

Puebla

et al. 1997

Molecular Brain Research

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“…The supernatant was then removed for assay of cyclic AMP (cAMP) by the method described by Gilman [12]. The SRIF concentration used was that deemed necessary to achieve inhibition of rat [39] AC activity. Similarly, forskolin was used at a concentration that could effectively stimulate the catalytic subunit of rat AC [39].…”

Section: Adenylyl Cyclase Assaymentioning

confidence: 99%

Activity of the hippocampal somatostatinergic system following daily administration of melatonin

Izquierdo-Claros

Boyano‐Adánez

Arilla‐Ferreiro

2004

Molecular Brain Research

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If melatonin or its analogs are to be used therapeutically in humans, their chronic effects on responsiveness of melatonin target cells need to be assessed. We have previously demonstrated that acute melatonin treatment regulates the somatostatinergic system in the rat hippocampus. In the present study, we have investigated the effects of subchronic and chronic daily treatment with melatonin on the somatostatinergic system in the rat hippocampus. Male Wistar rats (200 -250 g) were injected with melatonin (25 Ag/kg body weight, subcutaneously) daily for 4, 7 or 14 days and sacrificed 24 h after the last injection. Melatonin administration for 4 days induced a decrease in the hippocampal somatostatin (SRIF)-like immunoreactivity content as well as a decrease in the number of SRIF receptors and an increase in their apparent affinity. The decreased number of SRIF receptors in the melatonin (4 days)-treated rats was associated with a decreased capacity of SRIF to inhibit both basal and forskolin-stimulated adenylyl cyclase activity. These melatonin-induced effects reversed to control values after 7 or 14 days of treatment. Hippocampal membranes from control and melatonin-treated rats showed similar Gi and Gs activities. Melatonin treatment altered neither the functional Gi activity nor the Gia1 or Gia2 levels at any of the time periods studied. The present results suggest that chronic exposure to melatonin results in a tolerance of the hippocampus to this hormone. D

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“…The supernatant was taken for assay of cyclic AMP by the method of Gilman (28). The concentration of SS used was that necessary to achieve inhibition of rat (55) and human (5,27) brain AC. FK was used at a concentration that could effectively stimulate the AC catalytic subunit of rat brain (55).…”

Section: Adenylyl Cyclase Assaymentioning

confidence: 99%

“…The frontoparietal cortex contains high levels of SS and H 1 -histaminergic receptors (24,25,44,58,60,62). The SS receptors are coupled to the adenylyl cyclase (AC) enzyme system via the guanine nucleotide-binding inhibitory protein Gi (34,40,52,55). An H 1 -histamine receptor agonist, 2-pyridylethylamine (PEA) (22,65), and antagonist, mepyramine (22,65), were therefore used to examine the role of the H 1 -histamine receptors in the regulation of the SS receptor-effector system in rat frontoparietal cortical membranes.…”

mentioning

confidence: 99%

Histamine H1-Receptors Modulate Somatostatin Receptors Coupled to the Inhibition of Adenylyl Cyclase in the Rat Frontoparietal Cortex

1997

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Somatostatin inhibition of adenylate cyclase activity in different brain areas

Cited by 70 publications

References 30 publications

Influence of fluoxetine and p-chloroamphetamine on the somatostatin receptor–adenylyl cyclase system in the rat frontoparietal cortex

Influence of fluoxetine and p-chloroamphetamine on the somatostatin receptor–adenylyl cyclase system in the rat frontoparietal cortex

Activity of the hippocampal somatostatinergic system following daily administration of melatonin

Histamine H1-Receptors Modulate Somatostatin Receptors Coupled to the Inhibition of Adenylyl Cyclase in the Rat Frontoparietal Cortex

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