Somatostatin Effects on Cultured Human Fetal Epiphyseal Chondrocytes

Ferrández, M A; Carrascosa, Antonio; Audí, Laura; Ballabriga, A

doi:10.1203/00006450-199211000-00017

Cited by 15 publications

(5 citation statements)

References 17 publications

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“…There is evidence for direct local effects of SMS on cartilage, which are mostly inhibitive (36,37,38,39). In addition, SMS receptors were demonstrated in bone cells, which may mediate direct effects on the bone (40).…”

Section: Discussionmentioning

confidence: 99%

Progression of acromegalic arthropathy despite long-term biochemical control: a prospective, radiological study

Claessen

Ramautar

Pereira

et al. 2012

European Journal of Endocrinology

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Objective: Arthropathy is an invalidating complication of acromegaly, of which the prognosis and determinants are currently unknown in treated acromegaly. Therefore, the objective of the present study was to investigate the radiographic progression of arthropathy over a mean follow-up period of 2.6 years and determinants of outcome in patients with long-term, well-controlled acromegaly. Design: Prospective follow-up study. Methods: In a prospective cohort study we studied 58 patients (mean age 62, women 41%) with controlled acromegaly for a mean of 17.6 years. Radiographic progression of joint disease was defined by the Osteoarthritis Research Society International classification as a 1-point increase in joint space narrowing (JSN) or osteophyte scores on radiographs of the hands, knees, and hips obtained at the first study visit and after 2.6 years. Potential risk factors for progression were assessed. Results: Progression of osteophytes and JSN was observed in 72 and 74% of patients respectively. Higher age predisposed for osteophyte progression. Patients with biochemical control by somatostatin (SMS) analogs had more progression of osteophytosis than surgically cured patients (odds ratioZ18.9, PZ0.025), independent of age, sex, BMI, baseline IGF1 SDS and exon 3 deletion of the GHR. This was also evident for JSN progression, as were higher age and higher baseline IGF1 SDS. Conclusions: Acromegalic patients have progressive JSN and osteophytosis, despite long-term biochemical control. Parameters reflecting GH/IGF1 activity were associated with progressive joint disease. Remarkably, biochemical control by SMS analogs was associated with more progression than surgical cure. Although the present study is not a randomized controlled trial, this may indicate insufficient GH control according to current criteria and the need for more aggressive therapy.

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Section: Discussionmentioning

confidence: 99%

Progression of acromegalic arthropathy despite long-term biochemical control: a prospective, radiological study

Claessen

Ramautar

Pereira

et al. 2012

European Journal of Endocrinology

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“…Studies were carried out as previously described [24][25][26][27][28][29]. Primary confluent chondrocytes were trypsinized and plated in 96-microwell tissue culture plates at a density of 60,000 cells/cm 2 in 0.3 ml Ham F-12 serum-and antibiotic-free medium.…”

Section: H-thymidine Incorporation Into Dnamentioning

confidence: 99%

IGF-I and not IGF-II expression is regulated by glucocorticoids in human fetal epiphyseal chondrocytes

Fernández‐Cancio¹,

Estéban²,

Carrascosa³

et al. 2008

Growth Hormone & IGF Research

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“…The morbidity associated with the harvest and long-term consequences of articular cartilage harvest can be avoided with an apophyseal source. There are very few human in vitro studies on foetal epiphyseal chondrocytes which have looked at the hormonal influence on growth plate151617181920. None of the studies evaluated apophyseal chondrocyte expansion with BME for clinical translational purposes.…”

Section: Discussionmentioning

confidence: 99%

Bone marrow extract as a growth supplement for human iliac apophyseal chondrocyte culture

2016

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Background & objectives:Human bone marrow is rich in various growth factors which may support the chondrocyte growth. This study was conducted to compare the culture characteristics of human growth plate chondrocyte in foetal bovine serum (FBS) and human autologous bone marrow extract (BME) in monolayer culture.Methods:Iliac crest apophyseal cartilage was harvested from four donors, aged between two and nine years, undergoing hip surgery. Chondrocytes were propagated under two culture conditions, with 10 per cent FBS and 10 per cent autologous BME harvested from the same donors. Cells were harvested at 7, 14 and 21 days to assess viability, morphology, cell count and immunocytochemistry.Results:With an initial seeding density of 2500 cells/cm2, the average yield in monolayer cultured with FBS was 3.35 × 105, 5.9 × 105, 14.1 × 105 and BME was 0.66 × 105, 1.57 × 105 and 3.48 × 105 at 7, 14 and 21 days, respectively. Viability was 98.21 per cent with FBS and 97.45 per cent with BME at 21 days. In BME supplemented cultures, hyaline phenotype was maintained up to 21 days. The yield was higher in the FBS supplemented group; however, the phenotype could not be maintained by the FBS group as long as BME group.Interpretation & conclusions:Autologous BME was found to be a safer alternative to FBS for human studies. BME could maintain the hyaline phenotype for a longer time. Ways to enhance the cell yield needs to be explored in future studies.

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Somatostatin Effects on Cultured Human Fetal Epiphyseal Chondrocytes

Cited by 15 publications

References 17 publications

Progression of acromegalic arthropathy despite long-term biochemical control: a prospective, radiological study

Progression of acromegalic arthropathy despite long-term biochemical control: a prospective, radiological study

IGF-I and not IGF-II expression is regulated by glucocorticoids in human fetal epiphyseal chondrocytes

Bone marrow extract as a growth supplement for human iliac apophyseal chondrocyte culture

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