Somatostatin as a Regulator of First-Trimester Human Trophoblast Functions

Biondi, Carla; Ferretti, Maria Enrica; Lunghi, Laura; Medici, Silvia; Cervellati, Franco; Abelli, Luigi; Bertoni, Fabrizio; Adinolfi, Elena; Vesce, Fortunato; Bartolini, G; Papi, Alessio; D'Andrea, Sara; Berton, Stefania; Baldassarre, Gustavo

doi:10.1016/j.placenta.2008.05.009

Cited by 3 publications

(1 citation statement)

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“…Taking into account the difficulty to obtain first-trimester human trophoblast primary cultures, we utilized the HTR8/SVneo and Swan71 (Sw.71) cell lines, both were derived from human first trimester extravillous trophoblast (EVT)14 and have been employed as models of EVT cell proliferation, migration and invasiveness in vitro 15–18. In this study, we observed that both cell lines were susceptible to non-pandemic influenza A (H3N2) virus and fell into apoptosis without remarkable reduction of human leukocyte antigen (HLA)-G expression.…”

Section: Introductionmentioning

confidence: 99%

ORIGINAL ARTICLE: H3N2 Influenza A Virus Replicates in Immortalized Human First Trimester Trophoblast Cell Lines and Induces Their Rapid Apoptosis

Trinh

Izumi

Komine‐Aizawa

et al. 2009

American J Rep Immunol

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Problem Epidemiological data suggested that pandemic influenza increased the risks of spontaneous abortion and premature labor, while seasonal influenza also increased the risk of schizophrenia in adolescence. However, their pathogenesis is so far unknown. Method of study The first trimester trophoblast cell lines, namely, Swan71 and HTR8 cells were challenged with A/Udorn/72 influenza virus (H3N2). At indicated time points, cells were examined for expression of influenza proteins. Viral replication in culture media, apoptosis and the expression of human leukocyte antigen (HLA)-G were also examined. Results Intracellular localization of viral proteins was observed. Twenty-four hours after inoculation, virus was detected in culture media while most cells fell into apoptosis. During apoptosis, expression of HLA-G was unchanged. Conclusion We revealed replication of low pathogenic influenza virus in the first trimester trophoblast cell lines. Placental damages are likely to be induced by direct cytopathic effects of influenza virus and subsequent apoptosis rather than down regulation of HLA-G expression and subsequent rejection by maternal immune system.

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Section: Introductionmentioning

confidence: 99%