Somatostatin 28 and neuropeptide Y innervation in the septal area and related cortical and subcortical structures of the human brain. Distribution, relationships and evidence for differential coexistence

Gaspar, Patricia; Berger, Brigitte; Lesur, A.; Borsotti, J. P.; Febvret, A.

doi:10.1016/0306-4522(87)90197-7

Cited by 81 publications

(22 citation statements)

References 86 publications

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“…Moreover, the finding of a relatively high numerical density of NPYmRNAcontaining neurons in the nucleus accumbens ventrally to the anterior commissure is in agreement with findings on the peptide level, showing high NPY contents in this ventral area (Beal et al 1986a(Beal et al , b, 1987De Quidt and Emson 1986;Gaspar et al 1987). From these data taken together, it appears that the neurons observed in our study not only contain NPYmRNA but also produce the NPY peptide.…”

Section: Discussionsupporting

confidence: 94%

NPY-mRNA expressions in the nucleus accumbens, caudate putamen and cerebral cortex of apomorphine-susceptible and apomorphine-unsusceptible rats

Roubos

Spooren

Martens

et al. 2003

Cell and Tissue Research

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Using the apomorphine-induced stereotyped gnawing response as a selection criterion, two distinct groups of rats can be distinguished, apomorphine-susceptible (APO-SUS) and apomorphine-unsusceptible (APO-UNSUS) rats. These two lines differ in several components of both striatal and extrastriatal areas. This study deals with the expression of neuropeptide Y (NPY)mRNA-expressing neurons in the nucleus accumbens, caudate putamen and cerebral cortex of both rat lines, using non-radioactive in situ hybridisation. The morphology of the neurons in the three regions is similar, viz. oblong, rectangular or triangular, with two or three processes. The neurons are homogeneously distributed in all regions, and in the nucleus accumbens they are particularly numerous ventrally to the anterior commissure. Using automated image analysis, the mean numerical density of NPYmRNA-positive neurons per brain region and the mean NPYmRNA expression level per neuron per brain region were determined. No differences appear in the numerical densities of NPYmRNA-containing neurons in the nucleus accumbens, caudate putamen and cortex between APO-SUS and APO-UNSUS rats. However, distinct differences between the rat lines are present in the level of NPYmRNA expression per neuron in the nucleus accumbens and in the caudate putamen, showing that NPY contributes to the differential neurochemical make-up of these rat lines that is responsible for their obvious differences in behaviour, physiology and immune competence.

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Section: Discussionsupporting

confidence: 94%

NPY-mRNA expressions in the nucleus accumbens, caudate putamen and cerebral cortex of apomorphine-susceptible and apomorphine-unsusceptible rats

Roubos

Spooren

Martens

et al. 2003

Cell and Tissue Research

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“…Contrary to the cholinergic neurons these cells were selectively marked by the use of NADPH-d histochemistry (13,14). The coexistence of NADPH-d with SOM and NPY was also proved for neurons in other forebrain regions like olfactory bulb (4,11). In our ST preparations the single ChAT-immunostained and the NADPH-d stained cells are clearly distinguishable in accordance with the findings of foregoing authors mentioned (15,17) (Fig.…”

Section: Discussionsupporting

confidence: 89%

Alternate coexistence of NADPH-diaphorase with choline acetyltransferase or somatostatin in the rat neostriatum and basal forebrain.

Schober

Brauer

Luppa

1989

Acta Histochem. Cytochem

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The coexistence of reduced nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) with somatostatin (SOM) and choline acetyltransferase (ChAT) was compared between the rat neostriatum (ST) and basal forebrain complex (BF). NADPH-d coexists with SOM in ST, but not in the ventral pallidum (VP), in the substantia innominata-nucleus basalis complex (SI-NB) and in the magnocellular preoptic nucleus (NPM) of the BF. In the medial septal nucleus as well as in the vertical and the horizontal limbs of the diagonal band (MS-vDB, hDB) representing the rostral BF and in the SI-NB, a high number of ChATpositive cells also contain NADPH-d. The NPM shows a smaller number of double-stained cholinergic neurons. The functional significance of the NADPH-d coexistence in BF cholinergic cells is still unknown.Whereas the NADPH-d is known to coexist with SOM in a population of interneurons distributed throughout the rat ST, the cholinergic interneurons of this region constantly show negative reactions after treatment according to the NADPH-d histochemistry (15,17). In the BF, where the cholinergic cells represent the predominant neuronal component (2, 8) the occurrence of SOM containing cells is restricted to some parts of this unhomogeneous region (1). In the present study we follow the questions: a) Do SOM and ChAT immunoreactive neurons occur with coexisting NADPH-d in the rat BF? b) Are there regional differences in the distribution of double-and single-labelled cells in the BF? MATERIALS AND METHODSWistar rats were anaesthetized and perfused transcardially with a fixative of 4% paraformaldehyde in 0.1M phosphate buffer (PB), pH 7.4. After removing the brains an immersion fixation of 12hr followed. The forebrains were dissected out Address reprint requests to:

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“…Previously, aspiny neurons containing NPY were found in rats and humans Chronwall et al, 1985;Gaspar et al, 1987). These cells have also been shown to be in-nervated by GABA-ergic terminals (Massari et al, 1988).…”

Section: Discussionmentioning

confidence: 97%

Neuropeptide Y localization in telencephalic and diencephalic structures of the ground squirrel brain

et al. 1990

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The distribution of neuropeptide Y-immunoreactive (NPY-IR) perikarya, fibers, and terminals was investigated in the brain of two species of hibernatory ground squirrels, Spermophilus tridecemlineatus and S. richardsonii, by means of immunohistochemistry. In the telencephalic and diencephalic structures studied, distinct patterns of NPY-IR were observed which were essentially identical in male and female animals of both species. No differences in amount or distribution of NPY-IR structures were observed between animals which had been in induced hibernation for several months before sacrifice in March/April and those sacrificed one week after their capture in May. In some brain structures (e.g., the hypothalamic arcuate nucleus), IR cell bodies were observed only after pretreatment with colchicine. NPY-IR perikarya and fibers were found in the cerebral cortex, caudate nucleus-putamen, and dorsal part of the lateral septal nucleus. Dense fiber plexuses were seen in the lateral and medial parts of the bed nucleus of the stria terminalis. The numbers of IR perikarya observed in the medial part of the nucleus increased following intraventricular colchicine injections. The accumbens nucleus exhibited few IR cells and many fibers. Claustrum and endopiriform nuclei showed a considerable number of stained cells and fibers that increased in number and staining intensity in colchicine-treated ground squirrels. The induseum griseum showed a small band of IR cell bodies and varicose fibers. Bipolar of multipolar IR cells and varicose fibers were found in the basal nucleus of the amygdala. Dense fiber plexuses as well as IR terminals were seen in the median, medial, and lateral preoptic areas of the hypothalamus. Terminals and relatively few fibers were located in the periventricular, paraventricular, and supraoptic nuclei. The anterior, lateral, dorsomedial, and ventromedial hypothalamic nuclei contained relatively large numbers of terminals and fibers. In the suprachiasmatic nuclei, dense terminals were distributed mainly in the ventromedial subdivision. In the median eminence, immunoreactive terminals were concentrated in the external layer, with fibers predominant in the internal layer. NPY-IR perikarya were observed only in the arcuate nucleus of the hypothalamus and only following colchicine treatment. In the epithalamus (superficial part of the pineal gland and habenular nuclei), varicose fibers appeared mainly in perivascular locations (pineal) or as a dense plexus (habenular nuclei). These results from ground squirrels are discussed in comparison to those obtained in other species and with regard to considerations of the physiological role of NPY.

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Somatostatin 28 and neuropeptide Y innervation in the septal area and related cortical and subcortical structures of the human brain. Distribution, relationships and evidence for differential coexistence

Cited by 81 publications

References 86 publications

NPY-mRNA expressions in the nucleus accumbens, caudate putamen and cerebral cortex of apomorphine-susceptible and apomorphine-unsusceptible rats

NPY-mRNA expressions in the nucleus accumbens, caudate putamen and cerebral cortex of apomorphine-susceptible and apomorphine-unsusceptible rats

Alternate coexistence of NADPH-diaphorase with choline acetyltransferase or somatostatin in the rat neostriatum and basal forebrain.

Neuropeptide Y localization in telencephalic and diencephalic structures of the ground squirrel brain

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