Somatic Pairing: An Alternative for the Development of Cancer and other Hereditary Diseases.

Faruqi, Shamim A.; Miller, Richard C.; Noumoff, Joel S.

doi:10.1508/cytologia.59.439

Cited by 9 publications

(5 citation statements)

References 52 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gregory et al (1991) proposed a model explaining somatic recombination in their study of Prader-Willi syndrome and Woodage et al (1994) in Bloom syndrome. Faruqi et al (1994) on the basis of their studies on normal amniotic cells and blood, demonstrated not only mitotic pairing and chiasma formation but also provided the first objective proof of chromatid exchange and consequently validity to the alternate method of loss of heterozygosity rather than mutation in the etiology of cancers and other genetic diseases controlled by Mendelian inheritance of recessive alleles.…”

Section: Methodsmentioning

confidence: 98%

Evidence of Somatic Pairing in an Adenocarcinoma of the Lung.

1999

Self Cite

View full text Add to dashboard Cite

SummaryChromosomal analysis of an adenocarcinoma of the lung showed three clones. Additionally three cells were also recorded which showed mitotic chromosomal pairing involving varying number of chromosomes. In one of these cells almost the entire genome was involved in mitotic pairing of chromosomes. The importance of somatic pairing of chromosomes in the context of tumor evolution is highlighted.During the last decade, lung cancer (CA) has become the leading cause of deaths by cancer in both men and women. In the United States, lung cancer has an annual incidence of some 180,000cases with approximately 150,000 deaths. Unlike hematologic neoplasms where chromosomal aberrations correlate with diagnosis, management and prognosis, similar information regarding solid tumors is not yet recognized.We have made the observation in a case of adenocarcinoma of the lung which showed three different clones and also mitotic pairing of GTG banded homologous chromosomes.We have highlighted the significance of mitotic pairing of chromosomes in tumor evolution. Material and methodAfter excision, the tumor diagnosed as adenocarcinoma of the lung was brought in RPMI-1640 immediately to the laboratory. It was minced and exposed to 16 mg of collagenase in 10 ml . In addition to these clones, three cells in close proximity to each othcr showing somatic pairing among homologous chromosomes were observed on one of the slides. Chiasmata were absent in each of the chromosomal pairs. The cell with the maximum number of pairs showed chromosomal association of almost the entire genome (Fig. 1).

show abstract

Section: Methodsmentioning

confidence: 98%

Evidence of Somatic Pairing in an Adenocarcinoma of the Lung.

1999

Self Cite

View full text Add to dashboard Cite

show abstract

“…The recombinogenic capacity of aspartame was evaluated according to Franzoni et al (1997). Aspartame (Sigma) was added to sterile CM at 45°C.…”

Section: Techniques Of Genetic Analysismentioning

confidence: 99%

“…Pesticides and fungicides (Brownson et al, 1990;Baptista and Castro Prado, 1997), medicinal compounds (Holliday, 1964;Demopoulos et al, 1982;Franzoni et al, 1997) and industrial chemicals (Thomas et al, 1986;Hagmar et al, 1990) are worth mentioning.…”

Section: Introductionmentioning

confidence: 99%

“…Segregation of a recombinant and a parent chromatid towards the same mitotic pole will result in distal gene homozygosis at the point of exchange (Roper and Pritchard, 1955;Beumer et al, 1998). Recessive genes homozygosis, induced by mitotic crossing-over, may thus promote a neoplasm event in heterozygous cells, in the case of mutations in tumor suppressor genes (Faruqi et al, 1994;Laird and Jaenisch, 1996). Acta Scientiarum: Biological Sciences Maringá, v. 25, n. 1, p. 203-206, 2003 Agents recognized as neoplasm inducers are those that trigger the carcinogenesis process in initiating cells, even though incapable of separately inducing mutagenesis or carcinogenesis (Trosko et al, 1977;Lankas et al, 1977).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Segregação mitótica induzida pelo edulcorante L-Aspartil-L-Fenilalanina-Metil-Ester (aspartame) em células diplóides de Aspergillus nidulans

Gebara¹,

Gebara²,

Prado³

2003

Acta Sci. Biol. Sci.

View full text Add to dashboard Cite

ABSTRACT. This study aims to verify the recombinogenic effect of artificial edulcorant laspartil-l-phenylalanine-metyl-ester (aspartame), widely used in hypocaloric and lowglucose diets. Such effect may reflect cell response to genetic damage, induced by artificial edulcorant in the cell cycle G 2 period and shows its capacity to induce homozygosis of recessive genes, previously present in heterozygosis.

show abstract

“…Much evidence has emphasised that somatic recombination preponderantly promotes genome instability associated with carcinogenesis (Tiburi et al, 2002;Laird and Jaenisch, 1996;Faruqi et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide

et al. 2003

View full text Add to dashboard Cite

Ethidium bromide (EB) is an intercalating inhibitor of topoisomerase II and its activities are related to chemotherapeutic drugs used in anti-cancer treatments. EB promotes several genotoxic effects in exposed cells by stabilising the DNA-enzyme complex. The recombinagenic potential of EB was evaluated in our in vivo study by the loss of heterozygosity of nutritional markers in diploid Aspergillus nidulans cells through Homozygotization Index (HI). A DNA repair mutation, uvsZ and a chromosome duplication DP (II-I) were introduced in the genome of tested cells to obtain a sensitive system for the recombinagenesis detection. EB-treated diploid cells had HI values significantly greater than the control at both concentrations (4.0 x 10 -3 and 5.0 x 10 -3 µM). Results indicate that the intercalating agent is potentially capable of inducing mitotic crossing-over in diploid A. nidulans cells.

show abstract

Somatic Pairing: An Alternative for the Development of Cancer and other Hereditary Diseases.

Cited by 9 publications

References 52 publications

Evidence of Somatic Pairing in an Adenocarcinoma of the Lung.

Evidence of Somatic Pairing in an Adenocarcinoma of the Lung.

Segregação mitótica induzida pelo edulcorante L-Aspartil-L-Fenilalanina-Metil-Ester (aspartame) em células diplóides de Aspergillus nidulans

Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide

Contact Info

Product

Resources

About