Somatic outward currents in voltage clamp leech retzius nerve cell

Beleslin, B; Ristanović, Dušan; Osmanović, Smajo

doi:10.1016/0300-9629(88)91077-8

Cited by 10 publications

(13 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The method of dissection has been previously described [4] and complies with institutional research council guidelines. Dissected segments of 4 ganglia were immediately transferred to a 2.5 ml plastic chamber with a leech Ringer's solution (for the composition, see Solutions) and fixed by means of fine steel clips.…”

Section: Methodsmentioning

confidence: 99%

“…it is well known that action potential duration depends on the activity of ca 2+ -activated K + channels. in leech Retzius nerve cells, three classes of K + channels (fast, slow calcium-activated, and late voltage-regulated) have been identified [4]. The effect on outward currents was studied; this was shown in voltage-clamped neurons by long depolarizing steps (to 300 ms) from the holding potential, which was less than −40 mV in sodium-free leech Ringer solution, in order to induce fast and slow potassium outward currents without any current artefact.…”

Section: The Effects Of Chp and H 2 O 2 On The Action Potential Duratmentioning

confidence: 99%

See 1 more Smart Citation

A Comparison of the Effects of Cumene Hydroperoxide and Hydrogen Peroxide on Retzius Nerve Cells of the Leech <i>Haemopis sanguisuga</i>

Jovanović

2013

Exp. Anim.

View full text Add to dashboard Cite

Abstract:Oxidative stress and the production of reactive oxygen species are known to play a major role in neuronal cell damage, but the exact mechanisms responsible for neuronal injury and death remain uncertain. In the present study, we examined the effects of oxidative stress on spontaneous spike activity and depolarizing outward potassium current by exposing the Retzius neurons of the leech to cumene hydroperoxide (CHP) and hydrogen peroxide (H 2 O 2 ), the oxidants commonly used to examine oxidative mechanisms mediating cell death. We observed that relatively low concentrations of CHP (0.25, 1, and 1.5 mM) led to a marked prolongation of spontaneous repetitive activity. The prolonged action potentials showed an initial, spike-like depolarization followed by a plateau phase. In contrast, H 2 O 2 at the same and much higher concentrations (0.25 to 5 mM) did not significantly change the duration of spontaneous spike potentials of leech Retzius nerve cells (LRNCs). In the voltage clamp experiments, calcium-activated outward potassium currents, needed for the repolarization of the action potential, were suppressed with CHP, but not with H 2 O 2 . The present findings indicate that CHP is a more potent oxidant and neurotoxin than H 2 O 2 and that the effect of CHP on the electrophysiological properties of LRNCs may be due to the inhibition of the potassium channels.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: The Effects Of Chp and H 2 O 2 On The Action Potential Duratmentioning

confidence: 99%

A Comparison of the Effects of Cumene Hydroperoxide and Hydrogen Peroxide on Retzius Nerve Cells of the Leech <i>Haemopis sanguisuga</i>

Jovanović

2013

Exp. Anim.

View full text Add to dashboard Cite

show abstract

“…The dissection procedure, the recording method and point voltage clamp technique were employed as described previously (Beleslin et al, 1988). Dissected segments of 4 ganglia were immediately transferred to a 2.5 ml plastic chamber with a leech Ringer and fixed by means of fine steel clips.…”

Section: Methodsmentioning

confidence: 99%

Effects of Oxidative Stress on the Electrophysiological Function of Neuronal Membranes

Jovanović¹

2012

Oxidative Stress and Diseases

View full text Add to dashboard Cite

“…The experiments were performed utilizing Retzius nerve cells from the first ten abdominal ganglia of the leech, Haemopis sanguisuga (commonly known as the horse-leech). The dissection method was similar to that described previously (17,18). The 21 segmental ganglia contain ~400 neurons arranged in six packets.…”

Section: Experimental Animalsmentioning

confidence: 99%

“…Retzius neurons are the largest cells (~60 μm diameter of the soma) located on the ventral side of the ganglia. They were identified by their large cell bodies, position and firing properties (17,19). Isolated ganglia of the Retzius neurons have resting potentials that range from -40 to -60 mV and amplitude action potentials that range from 30 to 50 mV, and they fire spontaneously at a slow rate (0.2-3 APs/sec).…”

Section: Experimental Animalsmentioning

confidence: 99%

Effects of Divalent Cations on Outward Potassium Currents in Leech Retzius Nerve Cells

Jovanović

Mihaljevic

Kostić

2016

Serbian Journal of Experimental and Clinical Research

View full text Add to dashboard Cite

The present study examines the effects of divalent metals, cadmium (Cd2+) and manganese (Mn2+), on the outward potassium currents of Retzius cells in the hirudinid leeches Haemopis sanguisuga using conventional two-microelectrode voltageclamp techniques. The outward potassium current is activated by depolarization and plays an important role in determining both the neuronal excitability and action potential duration. A strong inhibition of the fast current and a clear reduction in the late currents of the outward current with 1 mM Cd2+ were obtained, which indicated that both components are sensitive to this metal. Complete blockage of the fast and partial reduction of the slow outward currents was observed after adding 1 mM Mn2+ to the extracellular fluid. These data show that the outward K+ current in leech Retzius nerve cells comprises at least two components: a voltage-dependent K+ current and a Ca2+- activated K+ current. These observations also indicate that Cd2+ is more eff ective than Mn2+ in blocking ion fl ow through these channels and that suppressing Ca2+-activated K+ outward currents can prolong the action potential in nerve cells.

show abstract

Somatic outward currents in voltage clamp leech retzius nerve cell

Cited by 10 publications

References 45 publications

A Comparison of the Effects of Cumene Hydroperoxide and Hydrogen Peroxide on Retzius Nerve Cells of the Leech <i>Haemopis sanguisuga</i>

A Comparison of the Effects of Cumene Hydroperoxide and Hydrogen Peroxide on Retzius Nerve Cells of the Leech <i>Haemopis sanguisuga</i>

Effects of Oxidative Stress on the Electrophysiological Function of Neuronal Membranes

Effects of Divalent Cations on Outward Potassium Currents in Leech Retzius Nerve Cells

Contact Info

Product

Resources

About