Somatic Hybridization in Solanum tuberosum × S. chacoense

Butenko, R. G.; Kuchko, A. A.

doi:10.1007/978-3-642-57945-5_12

Cited by 2 publications

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“…In potato, the first chemical fusion techniques were applied with S. tuberosum and S. chacoense (Butenko & Kuchko, 1980) or S. nigrum (Binding et al, 1982) as fusion parents, but they did not give any viable hybrids. The first hybrids from chemical fusions were obtained by Austin et al (1985).…”

Section: Introductionmentioning

confidence: 99%

Transfer of PLRV resistance fromSolanum verrucosum Schlechdt to potato (S. tuberosum L.) by protoplast electrofusion

et al. 2000

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SummarySomatic fusions between an accession of the diploid wild species Solanum verrucosum and a dihaploid S. tuberosum genotype were produced in order to incorporate resistance to potato leafroll virus (PLRV). In total 15 somatic hybrids out of 16 regenerants were Qbtained. Identification of hybrids was based on additive RAPD patterns, general morphological characteristics, chromosome numbers and chloroplast counts in stomata guard cells. A field trial was performed with the hybrids, their two parents and the control cultivar Kennebec to assess field performance and phenotypic variability. Yield parameters varied considerably among somatic hybrids. Some of the hybrids gave significantly higher yields, tuber numbers and tuber weights than both parents. Pollen fertility of hybrids ranged from 19 to 77%. Twelve hybrids were found to be resistant to PLRV.

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Section: Introductionmentioning

confidence: 99%

Transfer of PLRV resistance fromSolanum verrucosum Schlechdt to potato (S. tuberosum L.) by protoplast electrofusion

et al. 2000

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“…In Cucumis species, isolation and fusion of protoplasts from C. sativus , C. melo, and wild Cucumis species have been reported [23, 24]. Plants through protoplast fusion have been reported in the genus Brassica [25–27] and genus Solanum [24, 28, 29]. …”

Section: Introductionmentioning

confidence: 99%

Polyploidization Facilitates BiotechnologicalIn VitroTechniques in the GenusCucumis

Skálová

Ondřej

Doležalová

et al. 2010

Journal of Biomedicine and Biotechnology

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Prezygotic interspecific crossability barrier in the genus Cucumis is related to the ploidy level of the species (cucumber (C. sativus), x = 7; muskmelon (C. melo) and wild Cucumis species, x = 12). Polyploidization of maternal plants helps hybridization among other Cucumis species by overcoming prezygotic genetic barriers. The main objective of this paper is to compare the results of several methods supporting interspecific crosses in cucumber without and with polyploidization (comparison between diploid (2x) and mixoploid (2x/4x) cucumber maternal plants). Mixoploid plants were obtained after in vivo and in vitro polyploidization by colchicine and oryzalin. Ploidy level was estimated by flow cytometry. Embryo rescue, in vitro pollination, and isolation of mesophyll protoplast were tested and compared. Positive effect of polyploidization was observed during all experiments presented by higher regeneration capacity of cultivated mixoploid cucumber embryos, ovules, and protoplasts. Nevertheless, the hybrid character of putative hybrid accessions obtained after cross in vivo and in vitro pollination was not confirmed.

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Somatic Hybridization in Solanum tuberosum × S. chacoense

Cited by 2 publications

References 10 publications

Transfer of PLRV resistance fromSolanum verrucosum Schlechdt to potato (S. tuberosum L.) by protoplast electrofusion

Transfer of PLRV resistance fromSolanum verrucosum Schlechdt to potato (S. tuberosum L.) by protoplast electrofusion

Polyploidization Facilitates BiotechnologicalIn VitroTechniques in the GenusCucumis

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