Somatic hybridization in Citrus using embryogenic cybrid callus

Molecular characterization of the nuclear and cytoplasmic genomes of intergeneric diploid plants from cell fusion between Microcitrus papuana and Rough lemon

“…Subsequent analyses based on molecular markers of these plants have indicated that they are actually alloplasmic hybrids or cybrids. Most of these plants arose from interspecific combinations (Deng et al 1993Saito et al 1993Saito et al , 1994Yamamoto and Kobayashi 1995;Moriguchi et al 1997). Microcitrus confers resistance to both biotic stresses, including burrowing nematode and Phytophythora, and abiotic stresses such as drought and flood.…”

“…Many unexpected diploid plants have been regenerated following standard symmetric fusion between diploid species (Saito et al 1993(Saito et al , 1994Grosser et al 1996;Liu et al 1999. These plants were called leaf-parent-type ones because they were morphologically identical to the leaf parents used in the fusion.…”

Protoplast Fusion Technology – Somatic Hybridization and Cybridization

“…Since the first successful report on somatic hybridization with tobacco in 1972 [2], hundreds of reports have been published during the past three decades which extend the procedures to additional plant genera and evaluate the utilization potential of somatic hybrids in many crops, including rice, rapeseed, tomato, potato and citrus. Some key papers published during the evolution of this technology include those listed [3][4][5][6][7][8][9][10][11][12][13]. Plant somatic hybridization has been reviewed several times in general [3,[13][14][15] and, specifically, for citrus [16,17] and potato [18].…”

Production of Somatic Hybrids and Cybrids in the Rutaceae Family and Application to Citrus Breeding.

“…Furthermore we found regeneration of cybrids which possessed nuclear genomes of mesophyll cells and mitochondrial genomes of nucellar cells [11] . Here we describe establishment and application of Citrus somatic hybridization system, and utilization of cybrids for somatic hybridization [12].…”

“…The embryo-derived cybrid callus were embryogenic and applicable to the somatic hybridization system instead of nucellar callus [12] . Embryogenic callus of a lime type cybrid which possessed lime nuclear genome and sudachi mitochondrial genome were fused with lemon mesophyll cells and resulted in further production of somatic hybrid and cybrid plants.…”