Somatic hybridization between Lycopersicon peruvianum and L. pennellii: Regenerating ability and antibiotic resistance as selection systems

Adams, Tom L.; Quirós, Carlos F.

doi:10.1016/0168-9452(85)90205-5

Cited by 35 publications

(8 citation statements)

References 10 publications

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“…Somatic hybrid plants have been produced from interspecific combinations, L. peruvianum (+) L. pennellii (Adams and Quiros 1985;, L. esculentum (+) L. peruvianum (Kinsara et al 1986;Wijbrandi et al 1988;Ze1cer et al 1988;Ze1cer 1989), L. esculentum (+) L. pennelli Hanson 1985, 1987) and intergeneric combination L. esculentum (+) Solanum tuberosum (Me1chers et al 1978;Smillie et al 1979;Shepard et al 1983), L. peruvianum (+) Petunia hybrida (Tabaeizadeh et al 1985;, L. esculentum (+) S. rickii Hanson et al1987), L. esculentum (+) S.lycopersicoides (Handley et al 1986;, L. esculentum (+) S. integrifolium and L. escu/entum (+) S. muricatum (Ze1cer 1989; see also Chap. In addition, the transfer of cytoplasmically encoded traits (male sterility, herbicide and disease resistance localized on chloroplast or mitochondrial DNA) can potentially be realized using protoplast fusion.…”

Section: Somatic Hybrid Plantsmentioning

confidence: 99%

Genetic Improvement of Tomato

Kalloo¹

1991

Monographs on Theoretical and Applied Genetics

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Section: Somatic Hybrid Plantsmentioning

confidence: 99%

Genetic Improvement of Tomato

Kalloo¹

1991

Monographs on Theoretical and Applied Genetics

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“…Hybrids of these recessive mutants with nitrate reductase-positive genotypes will grow on media with nitrate as the sole nitrogen source . Adams & Quiros (1985) used a mutant with a dominant resistance against the antibiotic G418 as a marker for L. pennellii suspension culture protoplasts . Resistances against antibiotics such as kanamycin and hygromycin have also been introduced into the donor or recipient parents by Agrobacterium-mediated transformation .…”

Section: Protoplast Fusion Technologymentioning

confidence: 99%

Somatic hybridization as a tool for tomato breeding

et al. 1994

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Protoplast fusion can be used to produce somatic hybrids of species that cannot be obtained by sexual hybridization . The possibility to introgress genes from Solanum species into the cultivated tomato species Lycopersicon esculentum, and to obtain novel cytoplasm-nucleus combinations (cybrids) was considered as an important strategy to extend the genetic variation available for tomato breeding . Somatic hybrids between L. esculentum and other Lycopersicon species, as well as between L. esculentum and Solanum or Nicotiana species, have been produced . Specific mutants, genotypes with antibiotic resistances, and metabolic inhibition by iodoacetate or iodoacetamide and irradiation were used for the selection of hybrids . In addition, the improvement of protoplast culture techniques and the use of the favourable tissue culture traits derived from species such as L. peruvianum, which have been introduced into tomato by classical breeding, allowed the efficient recovery of somatic hybrids . However, the occurrence of somatic incongruity in fusion combinations of L. esculentum and Solanum and even more in L. esculentum and Nicotiana, did not allow the production of true cybrids and/or fertile hybrids, indicating the importance of both cytoplasm-nucleus and nucleus-nucleus interactions in somatic incongruity . Another problem with fusions between distantly related species is the strongly reduced fertility of the hybrids and the very limited homoeologous recombination between chromosomes of the parental species . Partial genome transfer from donor to recipient through microprotoplast (+) protoplast fusion, and the production of monosomic or disomic chromosome addition lines, might overcome some of these problems. In symmetric somatic hybrids between L. esculentum and S. tuberosum the occurrence of limited somatic and meiotic recombination was demonstrated . Fertile progeny plants could be obtained, though at a low frequency, when embryo rescue was performed on a large scale after backcrossing hexaploid somatic tomato (+) potato hybrids with a tetraploid potato genotype . The potential value of genomic in situ hybridization (GISH) and RFLPs for the analysis of the genome/chromosome composition of the hybrids has been demonstrated for intergeneric somatic hybrids between Lycopersicon and Solanum.

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“…Selection criteria thus far applied in fusion experiments are the capacity pf hybrids to regenerate (Adams & Quiros, 1985) ; Wijbrandi et al ., 1988), kanamycin resistance (Adams & Quiros, 1985), the ability to survive * Resistance was expressed at the callus level (also on callus derived from regenerated plants) but not at the plant level . Smith et al .…”

Section: Mutant Selection At the Cellular Levelmentioning

confidence: 99%

“…Also, chloroplast-encoded albino mutants (Hosticka & Kinshara et al ., 1986 ;Wijbrandi et al . 1988Melchers et al ., 1978, Shepard et al ., 1983Handley et al ., 1986Tan, 1987O'Connell et al ., 1985Adams & Quiros, 1985Tan, 1987Tabaeizadeh et al ., 1985Guri et al ., 1988 Specification glyphosate L-phosphinothricin Hanson, 1984) and chloroplast-encoded antibiotic resistances (Jansen et al, in preparation) are now available in tomato . Thus far, use of the somatic hybrids in breeding programs has not been reported .…”

Section: Mutant Selection At the Cellular Levelmentioning

confidence: 99%

Tomato: a crop species amenable to improvement by cellular and molecular methods

et al. 1989

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Tomato is a crop plant with a relatively small DNA content per haploid genome and a well developed genetics . Plant regeneration from explants and protoplasts is feasable which led to the development of efficient transformation procedures .In view of the current data, the isolation of useful mutants at the cellular level probably will be of limited value in the genetic improvement of tomato . Protoplast fusion may lead to novel combinations of organelle and nuclear DNA (cybrids), whereas this technique also provides a means of introducing genetic information from alien species into tomato . Important developments have come from molecular approaches . Following the construction of an RFLP map, these RFLP markers can be used in tomato to tag quantitative traits bred in from related species . Both RFLP's and transposons are in the process of being used to clone desired genes for which no gene products are known . Cloned genes can be introduced and potentially improve specific properties of tomato especially those controlled by single genes . Recent results suggest that, in principle, phenotypic mutants can be created for cloned and characterized genes and will prove their value in further improving the cultivated tomato .

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Somatic hybridization between Lycopersicon peruvianum and L. pennellii: Regenerating ability and antibiotic resistance as selection systems

Cited by 35 publications

References 10 publications

Genetic Improvement of Tomato

Genetic Improvement of Tomato

Somatic hybridization as a tool for tomato breeding

Tomato: a crop species amenable to improvement by cellular and molecular methods

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