Somatic Embryogenesis Protocol: Coffee (Coffea arabica L. and C. canephora P.)

Etienne, Hervé

doi:10.1007/1-4020-2985-3_14

Cited by 54 publications

(76 citation statements)

References 13 publications

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“…Fungal and bacterial contamination have been considered limiting factors to in vitro propagation of distinct species, such as Jatropha curcas L. (Vanzolini et al 2010), Ceiba pentandra L. Gaertn , Carica papaya L. (Silva et al 2007) and Coffea sp. (Maciel et al 2003, Etienne 2005. The occurrence of contamination directly depends on the phytosanitary conditions of explant donor plants (Etienne 2005) and of an accurate disinfestation of the explants.…”

Section: In Vitro Development Of Somatic Embryos and Plantlet Regenermentioning

confidence: 99%

“…(Maciel et al 2003, Etienne 2005. The occurrence of contamination directly depends on the phytosanitary conditions of explant donor plants (Etienne 2005) and of an accurate disinfestation of the explants.…”

Section: In Vitro Development Of Somatic Embryos and Plantlet Regenermentioning

confidence: 99%

“…In Coffea species, SE protocols are frequently based on media supplemented with distinct combinations of auxins and cytokinins, providing an indirect response (Berthouly and Etienne 1999, Maciel et al 2003, Cid et al 2004, Etienne 2005, Almeida et al 2008. Using leaf explants, Etienne (2005) developed a HFSE procedure with five culture stages for the mass in vitro propagation of coffee plantlets.…”

Section: In Vitro Development Of Somatic Embryos and Plantlet Regenermentioning

confidence: 99%

“…Using leaf explants, Etienne (2005) developed a HFSE procedure with five culture stages for the mass in vitro propagation of coffee plantlets. Owing to the high rate of somaclonal variation observed in this in vitro system, the same author reported that direct SE must be performed for germplasm conservation.…”

Section: In Vitro Development Of Somatic Embryos and Plantlet Regenermentioning

confidence: 99%

“…Similarly, Berthouly and Etienne (1999) evidenced a genotypic effect on the embryogenic response of C. canephora and C. arabica, which varied not only interspecifically, but also between distinct genotypes of a single species. In this sense, in vitro SE protocols are frequently modified with the aim of strictly adjusting the culture conditions to a particular Coffea genotype (Neuenschwander and Baumann 1992, Maciel et al 2003, Etienne 2005.…”

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confidence: 99%

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Regeneration of Allotriploid <i>Coffea</i> Plants from Tissue Culture: Resolving the Propagation Problems Promoted by Irregular Meiosis

2016

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Summary Híbrido de Timor (HT) is a natural interspecific hybrid of Coffea arabica L. with Coffea canephoraPierre ex Froehner that has played a substantial role in breeding programs as source of resistance genes. The original HT plant is represented by the anorthoploid accession CIFC 4106 , an allotriploid plant with 3x=33 chromosomes and 2C=2.10 pg. From this HT plant, other accessions have been obtained and used in crossings with C. arabica to provide resistant plants to the main pathogens. However, each HT accession and their derivates show a particular karyotype due to irregular anaphasic disjunction, which results in a few reproductive cells with an unbalanced number of chromosomes. Regarding this fact and the crop relevance of HT, this study aimed to develop a direct somatic embryogenesis system for the clonal propagation of this germplasm, using the first HT plant as explant donor. To accomplish this purpose, disinfested leaf explants of greenhouse-cultivated HT CIFC 4106 plants were inoculated in medium supplied with 0.001 g L 1 6-benzylaminopurine. Somatic embryogenesis process was asynchronous, with distinct developmental stages occurring simultaneously. Besides, emergence of secondary embryos from primary ones was observed. Mature embryos were germinated, and well-rooted embryos were selected for plantlet regeneration. The nuclear DNA content and the karyotype showed that allotriploidy was conserved in all regenerated plantlets. Considering these results, the direct somatic embryogenesis protocol adopted in the present work was imperative for the accurate clonal propagation of HT, being relevant for multiplication and conservation of elite accessions that show an irregular meiosis.

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Section: In Vitro Development Of Somatic Embryos and Plantlet Regenermentioning

confidence: 99%

Section: In Vitro Development Of Somatic Embryos and Plantlet Regenermentioning

confidence: 99%

Section: In Vitro Development Of Somatic Embryos and Plantlet Regenermentioning

confidence: 99%

Section: In Vitro Development Of Somatic Embryos and Plantlet Regenermentioning

confidence: 99%

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confidence: 99%

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Regeneration of Allotriploid <i>Coffea</i> Plants from Tissue Culture: Resolving the Propagation Problems Promoted by Irregular Meiosis

2016

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Coffee

Etienne

Alpizar

Lebailly

et al. 2008

Compendium of Transgenic Crop Plants

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Coffee is one of the world's most valuable export commodities and about 125 millions people depend on coffee for their live hood. The recent development of large coffee expressed sequence tag (EST) as well as the development of coffee bacterial artificial chromosome (BAC) libraries is leading to an enormous production of information in the area of coffee genomics. To take advantage of these resources, a key issue is the development and use of methods like genetic transformation to define gene functions at the molecular and organism level. The present chapter first describes the actual breeding and genomics strategies for the two cultivated species Coffea arabica and C. canephora . In a second part, all the methods and gene constructs used to develop transgenic coffees since the first work in 1991 are precisely analyzed. These methods include electroporation, biolistic delivery and Agrobacterium tumefaciens and A. rhizogenes ‐mediated transformation. Results of transgene expression for herbicide resistance, pathogen resistance and physiological traits are also presented. In the following part, the expected products of coffee transgenics are discussed. They deal with resistance to insect pests and nematodes, decaffeinated coffee, resistance to abiotic stresses, pathogen resistance, and improvement in cup quality characteristics and fruit ripening. Finally, the actual bottlenecks of coffee genetic transformation and the ongoing technology developments are described.

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Breeding Coffee (Coffea arabica) for Sustainable Production

Lebailly¹,

Bertrand²,

Etienne³

Breeding Plantation Tree Crops: Tropical Species

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Somatic Embryogenesis Protocol: Coffee (Coffea arabica L. and C. canephora P.)

Cited by 54 publications

References 13 publications

Regeneration of Allotriploid <i>Coffea</i> Plants from Tissue Culture: Resolving the Propagation Problems Promoted by Irregular Meiosis

Regeneration of Allotriploid <i>Coffea</i> Plants from Tissue Culture: Resolving the Propagation Problems Promoted by Irregular Meiosis

Coffee

Breeding Coffee (Coffea arabica) for Sustainable Production

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