Somatic embryogenesis of the hybrid Abies cilicica × Abies cephalonica

Korecký, J.; Vítámvás, Jan

doi:10.17221/54/2010-jfs

Cited by 4 publications

(2 citation statements)

References 30 publications

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“…In the genus Abies somatic embryogenesis has been initiated in several species, including A. alba (Schuller et al, 1989; Zoglauer and Reuther, 1996; Nawrot-Chorabik, 2008; Vooková and Kormutak, 2009), A. balsamea (Guevin et al, 1994), A. cephalonica (Krajňáková et al, 2008), A. fraseri (Guevin and Kirby, 1997; Pullmann et al, 2016), A. lasiocarpa (Kvaalen et al, 2005), A. nordmanniana (Nörgaard and Krogstrup, 1991; Misson et al, 2006; Nawrot-Chorabik, 2016), A. numidica (Vooková et al, 2001, Vooková et al, 2003), A. cilicica (Vooková and Kormut’ák, 2003), and hybrid firs (Gajdošová et al, 1995; 1997/1998; Korecký and Vitámvás, 2011). It is worthy of mention that in A. alba somatic embryogenesis was initiated as early as 1986 (Erdelský and Barančok, 1986).…”

Section: Somatic Embryogenesis In the Hybrids Abies Alba X A Cephalomentioning

confidence: 99%

Somatic Embryogenesis in Selected Conifer Trees Pinus nigra Arn. and Abies Hybrids

et al. 2019

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Somatic embryogenesis was achieved in the conifers Pinus nigra Arn. and in the hybrids Abies alba ×A. cephalonica and Abies alba ×A. numidica. For initiation of embryogenic tissue in P. nigra, immature zygotic embryos enclosed in megagametophytes were used. The initiated embryogenic cultures were maintained and proliferated on solid culture medium DCR supplemented with 9 μM 2,4-D and 2.2 μM BA. Microscopic investigations revealed the presence of bipolar early somatic embryos in proliferating tissue. Suspension cultures have also been established by resuspending the embryogenic tissue in liquid culture medium. Experimentation with abscisic acid concentration resulted in successful somatic embryo maturation. Besides abscisic acid, the carbohydrate content or higher concentration of gelling agent in maturation medium were also important requirements for somatic embryo maturation. Germination of cotyledonary somatic embryos occurred on hormone-free medium and terminated in somatic seedlings regeneration. The regenerated somatic seedlings were transferred to soil and were capable of successful development. For initiation of embryogenic tissue in Abies hybrids juvenile explants as immature or mature zygotic embryos as well as cotyledons were used and 4.4 μM BA as sole plant growth regulator was sufficient. Medium of the same composition was also suitable for their long-term maintenance. Maturation of somatic embryos was achieved on solid DCR medium supplemented with 38 μM abscisic acid, polyethylene glycol (0, 5, 7.5, and 10% PEG-4000) and different carbohydrates such as maltose, sucrose and glucose (each 3%). PEG-4000 stimulated somatic embryo development depending on the carbohydrate source used. Cotyledonary somatic embryos germinated after desiccation treatment and the regenerated somatic seedlings were transferred to soil. Cryopreservation of embryogenic tissue could be an alternative method for long-term maintenance. For cryopreservation the slow-freezing method was used with success. Tissue regeneration in the post thaw period was relatively high and the regenerated tissue produced mature somatic embryos and subsequent plantlets. The embryogenic tissue was also used in experiments focused on genetic transformation either by biolistic (P. nigra) or Agrobacterium-mediated (Abies hybrids) methods. A proteomic study was performed to gain a deeper insight into the early stages of P. nigra somatic embryogenesis.

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Section: Somatic Embryogenesis In the Hybrids Abies Alba X A Cephalomentioning

confidence: 99%

Somatic Embryogenesis in Selected Conifer Trees Pinus nigra Arn. and Abies Hybrids

et al. 2019

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“…The application of somatic embryogenesis led to new opportunties for micropropagtion of Abies species, with a focus on the initiation of embryogenic tissues from immature or mature zygotic embryos. In Abies nordmanniana [12], A. lasiocarpa [13], A. cephalonica [14] or some Abies hybrids [15][16][17][18], embryogenic tissue was initiated from immature zygotic embryos. Other members of the genus, such as A. balsamea, A. fraseri and A. procera, proved to be more recalcitrant toward somatic embryogenesis [19].…”

Section: Introductionmentioning

confidence: 99%

Physiological and Structural Aspects of In Vitro Somatic Embryogenesis in Abies alba Mill

Salaj

Klubicová

Panis

et al. 2020

Forests

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Initiation of somatic embryogenesis from immature zygotic embryos, long-term maintenance of embryogenic tissue in vitro or by cryopreservation, as well as maturation, of somatic embryos of Abies alba Mill. are reported in this study. For the initiation of embryogenic tissues, a DCR medium containing different types of cytokinins (1 mg.L−1) were tested. During three consecutive years, 61 cell lines were initiated out of 1308 explants, with initiation frequencies ranging between 0.83 and 13.33%. The type of cytokinin had no profound effect on the initiation frequency within one given year. Microscopic observations revealed presence of bipolar somatic embryos in all initiated embryogenic tissues. Besides the typical bipolar somatic embryos, huge polyembryonal complexes, as well as “twin” embryos, were observed. Maturation of somatic embryos occurred on a DCR medium supplemented by abscisic acid (10 mg.L−1), polyethylene glycol (PEG-4000, 7.5%) and 3% maltose. The maturation capacity was cell-line dependent. All of the four tested cell lines produced cotyledonary somatic embryos, though at different quantities, of 16 to 252 per g of fresh weight. After germination, seedlings developed, but their further growth soon stopped after the formation of a resting bud. Altogether, seven cell lines were cryopreserved, using the slow-freezing technique. After rewarming, all tested cell lines showed regrowth rates between 81.8 and 100%.

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Fraser Fir (Abies fraseri [Pursh] Poir.)

Pullman

Frampton

2018

Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants

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Somatic embryogenesis of the hybrid Abies cilicica × Abies cephalonica

Cited by 4 publications

References 30 publications

Somatic Embryogenesis in Selected Conifer Trees Pinus nigra Arn. and Abies Hybrids

Somatic Embryogenesis in Selected Conifer Trees Pinus nigra Arn. and Abies Hybrids

Physiological and Structural Aspects of In Vitro Somatic Embryogenesis in Abies alba Mill

Fraser Fir (Abies fraseri [Pursh] Poir.)

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