Somatic Embryogenesis of Dendrobium lasianthera X  Dendrobium antennatum with the Addition of BA and NAA

Sasmita, Heni Dwi; Dewanti, Parawita; Alfian, Firdha Narulita

doi:10.24831/jai.v50i2.39715

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References 5 publications

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“…The combination of shoot tips explant, half strength MS medium, and airlift bioreactor were applied for D. Zahra FR 62, D. Gradita 10, D. Indonesia Raya Ina, and D. Balithi CF22-58 Rachmawati et al, 2014Rachmawati et al, , 2016aRachmawati et al, , 2019Rachmawati et al, , 2022. While, the combination of basal part of plantlets as an explant, half strength MS medium, and airlift bioreactor for D. lasianthera x D. antennatum (Sasmita et al, 2022). However, there is no report on D. Syifa Agrihorti and D. Dian Agrihorti.…”

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confidence: 99%

Optimizing the potential utilization of bioreactors for the mass propagation of Indonesian Dendrobium varieties

RACHMAWATI,

PRAMANIK,

SHINTIAVIRA

et al. 2024

Not Bot Horti Agrobo

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The study focuses on two Indonesia Dendrobium varieties, D. Dian Agrihorti (DDA) and D. Syifa Agrihorti (DSA), which have potential to be commercialized, but face limitations on the availability of qualified seedlings sustainably. The research aimed to establish an in vitro propagation protocol using a Temporary Immersion System (TIS) to produce high-quality seedlings efficiently. Various factors, including varieties, media, plant growth regulators, anti-phenol compounds, organic additives, and TIS settings, were investigated using the basal part of the plantlet as the explant source. Key findings revealed that DDA outperformed DSA across all observed variables. In the initiation phase, basal plantlets cultured in Murashige and Skoog (MS) medium supplemented with 1.0 mg L-1 thidiazuron and 0.5 mg L-1 N-6 benzylaminopurine enhanced embryogenic callus (EC) formation, with a 13.5-day initiation period, 72% potential explant growth, 0.41 cm callus size, and a 3.45 rate of multiplication. During the proliferation stage, the addition of 150 mg L-1 ascorbic acid (AC) and the application of a TIS with a 30-minute dry period and a 15-minute wet period resulted in a 515.5% increase in EC fresh weight for DDA accompanied by a 6.16 multiplication rate. Regeneration of shoots was achieved using a Vacin and Went medium with 150 g L-1 banana extract, yielding 29.2 shoots per clump. Subsequent rooting of the shoots in 2 g L-1 Hyponex® medium with 20 g L-1 sugar and 2% AC proved successful. Acclimatization of plantlets with Cycas rumpii bulk demonstrated a 100% survivability rate. The established propagation protocol for DDA holds significant potential for application to other Dendrobium varieties, offering a sustainable and efficient method for meeting commercial demands in the Indonesian market.

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