Somatic Embryogenesis in Pines

Castander-Olarieta, Ander; Moncaleán, Paloma; Montalbán, Itziar A.

doi:10.1007/978-1-0716-2485-2_4

Cited by 10 publications

(5 citation statements)

References 80 publications

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“…In our lab, several studies have been developed to optimize somatic embryogenesis [ 18 ] and organogenesis [ 19 , 20 ] in many species of Pinus . Moreover, as a result of priming cells during the somatic embryogenesis process in conifers, different metabolic, epigenetic, and proteomic profiles related to abiotic stress tolerance have been described recently [ 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

Rojas-Vargas

Castander-Olarieta²,

Nascimento³

et al. 2023

Plants

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Pinus. ponderosa (P. Lawson and C. Lawson) is a commercial tree and one of the most important forest species in North America. Ponderosa pine suffers hardship when going through vegetative propagation and, in some cases, 15–30 years are needed to achieve full reproductive capacity. Based on previous works on P. ponderosa regeneration through in vitro organogenesis and trying to improve the published protocols, our objective was to analyze the influence of different types of explants, basal culture media, cytokinins, auxins, and light treatments on the success of shoot multiplication and rooting phases. Whole zygotic embryos and 44 µΜ 6-benzyladenine showed the best results in terms of explants survival. For shoot organogenesis, whole zygotic embryos and half LP (LP medium, Quoirin and Lepoivre, 1977, modified by Aitken-Christie et al., 1988) macronutrients were selected. A significant positive interaction between whole zygotic embryos and half LP macronutrients was found for the percentage of explants forming shoots. Regarding the light treatments applied, a significantly higher percentage of shoots elongated enough to be rooted was detected in shoots growing under blue LED at a light intensity of 61.09 µmol m−2 s−1. However, the acclimatization percentage was higher in shoots previously cultivated under fluorescent light at a light intensity of 61.71 µmol m−2 s−1. Anatomical studies using light microscopy and scanning electron microscopy showed the light treatments promoted differences in anatomical aspects in in vitro shoots; needles of plantlets exposed to red and blue LEDs revealed less stomata compared with needles from plantlets exposed to fluorescent light.

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Section: Introductionmentioning

confidence: 99%

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

Rojas-Vargas

Castander-Olarieta²,

Nascimento³

et al. 2023

Plants

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“…The culture media employed for tissue recovery can be those used for initiation or proliferation at SE initial stages ( Castander-Olarieta et al., 2022 ) or media with the same content of sugar or sugar alcohol as in the pre-treatment of tissues but in reverse order ( Varis et al., 2017 ). Some authors have found a nurse culture helpful to regenerate the thawed tissues ( Hargreaves et al., 2002 ).…”

Section: Cryopreservation Of Embryogenic Materials In Woody Speciesmentioning

confidence: 99%

Current status of the cryopreservation of embryogenic material of woody species

Ballesteros,

Martínez,

Sánchez-Romero

et al. 2024

Front. Plant Sci.

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Cryopreservation, or the storage at liquid nitrogen temperatures (-196°C), of embryogenic cells or somatic embryos allows their long-term conservation without loss of their embryogenic capacity. During the last decade, protocols for cryopreservation of embryogenic material of woody species have been increasing in number and importance. However, despite the large experimental evidence proved in thousands of embryogenic lines, the application for the large-scale conservation of embryogenic material in cryobanks is still limited. Cryopreservation facilitates the management of embryogenic lines, reducing costs and time spent on their maintenance, thus limiting the risk of the appearance of somaclonal variation or contamination. Somatic embryogenesis in combination with cryopreservation is especially useful to preserve the juvenility of lines while the corresponding clones are being field-tested. Hence, when tree performance has been evaluated, selected varieties can be propagated from the cryostock. The traditional method of slow cooling or techniques based on vitrification are mostly applied procedures. For example, slow cooling methods are widely applied to conserve embryogenic lines of conifers. Desiccation based procedures, although simpler, have been applied in a smaller number of species. Genetic stability of the cryopreserved material is supported by multiloci PCR-derived markers in most of the assayed species, whereas DNA methylation status assays showed that cryopreservation might induce some changes that were also observed after prolonged subculture of the embryogenic lines. This article reviews the cryopreservation of embryogenic cultures in conifers, fruit species, deciduous forest species and palms, including a description of the different cryopreservation procedures and the analysis of their genetic stability after storage in liquid nitrogen.

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“…Furthermore, the combined study of nuclear and chloroplast proteomes revealed the importance of proteins related to retrograde and anterograde signaling and to RNA metabolism rearrangement mediated by microRNAs, revealing a new layer of regulation in heat stress response ( Lamelas et al., 2022 ). Proteomic studies on heat stress in somatic embryos have found some commonalities in the proteins and processes involved, opening the door to the production of thermo-primed plants ( Castander-Olarieta, 2021 ; Castander-Olarieta et al., 2022 ).…”

Section: Proteomics Survey (2012-2022): Where Are We Now?mentioning

confidence: 99%

Proteomics research in forest trees: A 2012-2022 update

et al. 2023

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This review is a compilation of proteomic studies on forest tree species published in the last decade (2012-2022), mostly focused on the most investigated species, including Eucalyptus, Pinus, and Quercus. Improvements in equipment, platforms, and methods in addition to the increasing availability of genomic data have favored the biological knowledge of these species at the molecular, organismal, and community levels. Integration of proteomics with physiological, biochemical and other large-scale omics in the direction of the Systems Biology, will provide a comprehensive understanding of different biological processes, from growth and development to responses to biotic and abiotic stresses. As main issue we envisage that proteomics in long-living plants will thrive light on the plant responses and resilience to global climate change, contributing to climate mitigation strategies and molecular breeding programs. Proteomics not only will provide a molecular knowledge of the mechanisms of resilience to either biotic or abiotic stresses, but also will allow the identification on key gene products and its interaction. Proteomics research has also a translational character being applied to the characterization of the variability and biodiversity, as well as to wood and non-wood derived products, traceability, allergen and bioactive peptides identification, among others. Even thought, the full potential of proteomics is far from being fully exploited in forest tree research, with PTMs and interactomics being reserved to plant model systems. The most outstanding achievements in forest tree proteomics in the last decade as well as prospects are discussed.

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Somatic Embryogenesis in Pines

Cited by 10 publications

References 80 publications

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

Current status of the cryopreservation of embryogenic material of woody species

Proteomics research in forest trees: A 2012-2022 update

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