Somatic Embryogenesis in Mature Quercus Robur Trees

Toribio, M.; Fernández, Cristina E.; Celestino, C.; Martínez, M. T.; San-José, M. C.; Viéitez, A. M.

doi:10.1023/b:ticu.0000009245.92828.26

Cited by 48 publications

(26 citation statements)

References 13 publications

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“…However, during the last decade, important advances have been made in this area, and other explant types (e.g., leaves, apices, primordial shoots) derived from older trees were used to induce this process. Toribio et al (2004) achieved somatic embryo induction from leaves derived from mature hundred-year-old trees of Quercus robur. Although the frequency was low (0.3-3.6 depending on genotype and collection date), a large number of somatic embryos were obtained by secondary embryogenesis.…”

Section: Micropropagation By Somatic Embryogenesismentioning

confidence: 99%

“…Somatic embryogenesis is divided into several steps: culture initiation, proliferation, maturation and in vitro germination. It is induced from various explant types, such as immature or mature embryos obtained from seeds, buds, needles, leaves (Toribio et al, 2004;Nawrot-Chorabik, 2012) or shoot apex explants (San-José et al, 2010). Some explants regenerate a specific tissue type (embryogenic tissue) in the presence of plant growth regulators (usually cytokinins and/or auxins).…”

Section: Micropropagation By Somatic Embryogenesismentioning

confidence: 99%

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Tree somatic embryogenesis in science and forestry

Hazubska-Przybył

Bojarczuk

2016

Dendrobiology

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Somatic embryogenesis is the latest, and potentially the most efficient, method for the vegetative micropropagation of plants. Over the past three decades, numerous laboratory studies have investigated somatic embryogenesis of forest trees, yielding positive results for a number of economically important tree species. The first test trials were run and plantations were planted with interior spruce in the 90s by CellFor Inc. (Canada). However, at the beginning of the XXI century, the program to produce spruce and Douglas fir somatic seedlings was stopped for economic reasons. Thus, currently no operational program is ongoing except on a small scale in New Brunswick. In order to integrate somatic embryogenesis technology into operational reforestation programs, the production costs of forest tree somatic seedlings needs to be reduced, and the awareness of foresters and forest landowners that the material obtained through somatic embryogenesis is valuable needs to be increased. This awareness would enable implementation of this technology on a large scale for production and forest management throughout Europe including Poland. In this review, the importance of somatic embryogenesis in scientific research and in global and European forestry is presented. Our main aims are to provide basic information on the challenges in researching somatic embryogenesis of forest trees and to raise interest in this tree propagation technique in both scientists and foresters.

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Section: Micropropagation By Somatic Embryogenesismentioning

confidence: 99%

Section: Micropropagation By Somatic Embryogenesismentioning

confidence: 99%

Tree somatic embryogenesis in science and forestry

Hazubska-Przybył

Bojarczuk

2016

Dendrobiology

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“…Line T2H was of juvenile origin (Cuenca et al 1999) and lines B13, B17, and Sainza were initiated from Q. robur trees of between 100 and 300 years old (Toribio et al 2004;Martínez et al 2008). These embryogenic lines were maintained by secondary embryogenesis with serial subculture at 5-week intervals (Valladares et al 2006).…”

Section: Plant Materials and Culture Conditionsmentioning

confidence: 99%

High-efficiency Agrobacterium-mediated transformation in Quercus robur: selection by use of a temporary immersion system and assessment by quantitative PCR

Mallón

Viéitez

Vidal

2013

Plant Cell Tiss Organ Cult

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As the limiting factor for recovery of plants from oak embryogenic lines is the low embryo conversion rate, axillary shoot lines were established from transformed germinated embryos.Transformed embryos and shoots were cultured in medium with or without kanamycin and the responses to several morphogenetic processes (recovery after cryopreservation, germination, shoot proliferation, and rooting) were evaluated.2

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“…Singh et al (2002) En la actualidad, el establecimiento y estandarización de protocolos in vitro con tejidos de alta respuesta de regeneración en especies leñosas se considera fundamental para procesos de micropropagación masiva y para facilitar la tecnología de transformación genética. En varias especies frutales y forestales se han hecho estudios para obtener plantas in vitro por medio de organogénesis indirecta (Pérez-Tornero et al, 2000) y por embriogénesis somática (Toribio et al, 2004). Investigaciones efectuadas por Loh y Rao (1989), Amin y Jaiswal (1988), Ramírez y Salazar (1997), Pérez-Tornero et al (2000) indican que la propagación in vitro de guayaba por brotes adventicios es posible.…”

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Propagación in vitro de Psidium guajaba mediante organogénesis directa a partir de segmentos nodales

Ocampo

Núñez

2007

CTA

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Se indujeron múltiples brotes mediante organogénesis directa a partir de segmentos nodales de 10 genotipos diferentes de guayaba. Para ello se estableció un sistema de propagación clonal in vitro combinado con inducción rápida de brotes ex vitro para propagar árboles élite. La utilización de segmentos nodales permitió obtener en poco tiempo brotes adventicios adecuados para multiplicación masiva. La respuesta in vitro de los genotipos fue evaluada usando los medios de cultivo MS (Murashige y Skoog, 1962), Mc (Mascarenhas) y WPM (Woody Plant Medium) suplementados con 0,1 mg•L-1 de ácido indolácetico (AIA) y 0,25 mg•L-1 de bencilaminopurina (BAP). El procedimiento de desinfección con hipoclorito de sodio previno eficientemente la contaminación de los explantes después de la inoculación en el medio de cultivo. El mayor porcentaje en la inducción de brotes se logró con 0,25 mg•L-1 de BAP. Los segmentos nodales presentaron de 1 a 2 brotes adventicios por explante después de 15 días de inoculados y de 3 a 7 brotes a los 30 días después del inicio del cultivo. Una vez individualizados los brotes se usaron en una nueva fase de multiplicación masiva en la que se probaron cuatro sustratos diferentes durante el enraizamiento y el endurecimiento. Esta metodología permitió la propagación in vitro de guayaba cuatro semanas después del inicio del cultivo. Los mejores resultados se lograron con el medio WPM que permitió obtener las primeras plántulas enraizadas dos semanas después de la transferencia al sustrato de enraizamiento. In vitro propagation of Psidium guajaba using direct organogenesis from nodal segmentsMultiple shoots were induced using direct organogenesis from nodal segments of 10 different genotypes of guayaba. For this an in vitro clonal propagation system combined with rapid ex vitro induction of shoots was established in order to propagate elite trees. The use of nodal segments resulted in adventitious shoots adequate for mass multiplication in less time. The in vitro response of the genotypes was evaluated using the culture media MS (Murashige and Skoog, 1962), Mc (Mascarenhas) and WPM (Woody Plant Medium) supplemented with 0.1 mg·L-1 of indole acetic acid (IAA) and 0.25 mg·L-1 of benzoaminopurine (BAP). The procedure for sterilizing with sodium hypochlorite effectively prevented the contamination of the explants after the inoculation of the culture medium. The greatest percentage of shoot induction was achieved with 0.25 mg·L-1 of BAP. The nodal segments showed between 1-2 adventitious shoots per explant 15 days post-inoculation and 3-7 shoots 30 days post-inoculation. Once individualized, the shoots were used in a new mass multiplication phase in which four different substrates were tested during rooting and hardening. This methodology permitted the in vitro propagation of guayaba four weeks post-inoculation. The best results were achieved with the WPM medium that resulted in the first rooted plantlets two weeks after the transfer to the rooting substrate.

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Somatic Embryogenesis in Mature Quercus Robur Trees

Cited by 48 publications

References 13 publications

Tree somatic embryogenesis in science and forestry

Tree somatic embryogenesis in science and forestry

High-efficiency Agrobacterium-mediated transformation in Quercus robur: selection by use of a temporary immersion system and assessment by quantitative PCR

Propagación in vitro de Psidium guajaba mediante organogénesis directa a partir de segmentos nodales

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