Somatic Embryogenesis in Carica Papaya Through Zygotic Embryo Derived Callus Culture

Ramaswamy, A.; Phap, Phan Dinh; Soorianathasundaram, K.; Kumar, Naveen; Thirugnanakumar, S.; Sudhakar, D.; Balasubramanian, P.

doi:10.17660/actahortic.2010.851.29

Cited by 3 publications

(3 citation statements)

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“…Somatic embryo induction from zygotic embryo in papaya cv. Co.-7 using lower concentrations of 2,4-D also reported [17]. Somatic embryo induction from zygotic embryo was found using 2,4-D alone with 30g/l and also 60g/l sucrose concentrations [12].…”

Section: Resultsmentioning

confidence: 81%

Somatic Embryogenesis and Plant Regeneration from Zygotic Embryo in <i>Carica papaya</i> L., cv. Red-Lady

Kabir¹

2016

PLANT

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Somatic embryogenesis creates a number of opportunities to facilitate large-scale propagation, synthetic seed production, genetic improvement through somaclonal variation, in vitro mutagenesis, protoplast fusion and genetic transformation. Induction of somatic embryogenesis from the vegetative parts of papaya plant was met with low success rates and a slow process of regeneration. Success depends on the choice of explants, the species being used and on various methods of embryogenesis will study. The most suitable explant for somatic embryogenesis is a large portion of either meristematic tissue or cell that retain an ability to express totipotency. Various investigations were made on somatic embryo induction of Carica spp. such as ovules of immature fruits, zygotic embryos from immature fruits, axillary buds, peduncles of immature fruits etc. In this study, somatic embryo induction from zygotic embryo of an immature fruit was examined. Various concentrations of 2,4-D and two levels of sucrose concentrations with or without addition of glutamine were assessed to determine the best response for induction of somatic embryogenesis from immature zygotic embryo of immature fruits in papaya cv. Red-lady (Carica papaya L.). The results showed that MS medium supplemented with 5.0 mg/l 2,4-D + 400 mg/l glutamine + 60 g/l sucrose promoted the formation of highest (70%) percentage of somatic embryo and also the average maximum number (35.80±4.40) of somatic embryo per explant. The highest (44%) percentage of somatic embryo germination into complete plantlets were obtained on MS medium devoid of plant growth regulators. The embryo developed only shoots of 22% and only roots of 10% instead of complete plantlet formation in this medium of MS0. The average highest (4.25 ± 0.32 cm) shoot length per germinated somatic embryo derived complete plantlets were also achieved on MS0 medium. The maximum (90%) percentage of root induction and average highest (7.50 ± 0.75) number of root per somatic embryo derived shoot and also the average maximum (5.20 ± 0.40 cm) root length were obtained on MS medium without plant growth regulators. The survival rate of somatic embryo derived plantlets in the field was about 10% to 50% depending on management practices.

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Section: Resultsmentioning

confidence: 81%

Somatic Embryogenesis and Plant Regeneration from Zygotic Embryo in <i>Carica papaya</i> L., cv. Red-Lady

Kabir¹

2016

PLANT

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“…Castillo et al (1998) compared the maturation and subsequent conversion to plants of C. papaya L. 'Solo' and found that embryogenic calli cultured in a liquid phase maturation medium resulted in a significantly higher frequency of somatic embryogenesis (SE) and regeneration than treatments on agar-solidified medium. Anandan et al (2010) reported that suspension cultures (liquid medium) of papaya 'Co7' were superior to static cultures (semi-solid medium) for the maturation of somatic embryos.…”

Section: Experiments 2 -Malt Extract On 'Golden Thb' Papaya Somatic Emmentioning

confidence: 99%

The effects of an osmoregulator, carbohydrates and polyol on maturation and germination of ‘Golden THB’ papaya somatic embryos

Chagas

Cipriano

Lopes

et al. 2018

An. Acad. Bras. Ciênc.

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This study evaluated the effect of osmoregulators and carbohydrates on the maturation and germination of somatic embryos of papaya 'Golden THB'. Cotyledon explants from papaya seedlings germinated in vitro on basal MS medium were cultured on somatic embryogenesis induction medium (IM) containing MS salts, myo-inositol, sucrose, agar and p-chlorophenoxyacetic acid. After 50 days, embryogenic calli were transferred onto maturation media (MM) for 45 additional days. For experiment 1, a MS-based medium supplemented with abscisic acid, activated charcoal and concentrations of PEG 6000 (0; 40; 50; 60 and 70 g L-1) was used, whereas for experiment 2 malt extract concentrations (0; 0.1; 0.2; 0.3 and 0.4 g L-1) were assessed. The normal cotyledonary somatic embryos produced in experiment 2 were transferred to the germination medium (GM). The GM consisted of full-strength MS medium, sucrose, agar and was supplemented with myo-inositol at varying concentrations (0; 0.275; 0.55 and 0.825 mM). The PEG concentrations tested impaired the maturation of 'Golden THB' papaya somatic embryos. The MM, supplemented with malt extract at 0.153 g L-1 , promoted the greatest development of normal somatic embryos (18.28 SE calli-1), that is, two cotyledonary leaves produced 36.56 SE calli-1. The supplementation with 0.45 mM myo-inositol provided the highest germination percentage (47.42%) and conversion to emblings.

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“…AGRIES, v. 9, Ed. Especial, e023012, 2023 O mamão (Carica papaya L.) é uma frutífera cultivada em regiões tropicais e subtropicais de rápido crescimento, capaz de produzir durante o ano todo (Anandan et al, 2010). A maior parte da produção desta cultura está concentrada no norte do Estado do Espírito Santo e sul da Bahia (EMBRAPA, 2018), sendo nos plantios comerciais brasileiros, o método de propagação mais utilizado é via sementes (Oliveira e Trindade, 2000).…”

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Desenvolvimento Inicial De Variedades De Mamão Com Insumos Biológicos

Silva,

Medeiros,

Rosa

et al. 2023

Agri-Environ Sci

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O mamão (Carica papaya L.) é uma frutífera cultivada em regiões tropicais e subtropicais de rápido crescimento, e com produção durante o ano todo. A utilização de microrganismos como promotores de crescimento em frutíferas é uma estratégia promissora para incrementar a produtividade da cultura. Avaliar o desenvolvimento inicial de variedades de mamão com insumos biológicos. Utilizou-se delineamento inteiramente casualizado no esquema fatorial 2 x 6, sendo duas variedades de mamão (Formosa e Papaya), seis tratamentos e cinco repetições. Os tratamentos foram: testemunha, Nemax Ultra ® (substâncias húmicas e Trichoderma sp.); Biomaphos® (Bacillus subtilis e B. megaterium); 0,5% Nemax Ultra®+ Biomaphos®; 1% Nemax Ultra®+ Biomaphos®; 2% Nemax Ultra®+ Biomaphos®. As variáveis analisadas foram número total de folha, diâmetro do caule, comprimento do caule e da raiz e altura da parte aérea. Avaliou-se também a massa fresca total da parte aérea e da raiz, massa seca da parte aérea e da raiz. Os resultados obtidos demonstram que, na interação entre as variedades e os tratamentos com insumos biológicos, as variedades dentro das possibilidades de uso dos insumos biológicos influenciaram, significativamente, as variáveis altura de planta, comprimento do caule e comprimento da raiz. As variações observadas nos resultados das variáveis massa fresca da parte aérea e da raiz, bem como massa seca da parte aérea e da raiz, foram notavelmente elevadas, o que inviabilizou a apresentação dos dados obtidos. A utilização de insumos biológicos, de forma combinada, na produção de mudas de mamão contribui para o incremento das características morfológicas da fruteira.

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Somatic Embryogenesis in Carica Papaya Through Zygotic Embryo Derived Callus Culture

Cited by 3 publications

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Somatic Embryogenesis and Plant Regeneration from Zygotic Embryo in <i>Carica papaya</i> L., cv. Red-Lady

Somatic Embryogenesis and Plant Regeneration from Zygotic Embryo in <i>Carica papaya</i> L., cv. Red-Lady

The effects of an osmoregulator, carbohydrates and polyol on maturation and germination of ‘Golden THB’ papaya somatic embryos

Desenvolvimento Inicial De Variedades De Mamão Com Insumos Biológicos

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Somatic Embryogenesis in Carica Papaya Through Zygotic Embryo Derived Callus Culture

Cited by 3 publications

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Somatic Embryogenesis and Plant Regeneration from Zygotic Embryo in &lt;i&gt;Carica papaya&lt;/i&gt; L., cv. Red-Lady

Somatic Embryogenesis and Plant Regeneration from Zygotic Embryo in &lt;i&gt;Carica papaya&lt;/i&gt; L., cv. Red-Lady

The effects of an osmoregulator, carbohydrates and polyol on maturation and germination of ‘Golden THB’ papaya somatic embryos

Desenvolvimento Inicial De Variedades De Mamão Com Insumos Biológicos

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Somatic Embryogenesis and Plant Regeneration from Zygotic Embryo in <i>Carica papaya</i> L., cv. Red-Lady

Somatic Embryogenesis and Plant Regeneration from Zygotic Embryo in <i>Carica papaya</i> L., cv. Red-Lady