Somatic embryogenesis from stem and leaf explants of Quercus robur L.

Cuenca, B.; San-José, M. C.; Martínez, M. T.; Ballester, A.; Viéitez, A. M.

doi:10.1007/s002990050618

Cited by 67 publications

(48 citation statements)

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“…Thus, in E. nitens, accelerated cell division induced by hormonal treatments produced caulogenesis in compact calli; while in E. globulus, caulogenesis is produced from nodular calli (Bandyopadhyay et al, 1999). Likewise, indirect embrygenesis often occurs based on friable calli produced from leaf segments and stems of Quercus robur L. (Cuenca et al, 1999).…”

Section: Resultsmentioning

confidence: 99%

Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)

Avilés¹,

Ríos²,

González³

et al. 2009

Chilean J. Agric. Res.

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To define preliminary aspects of walnut (Juglans regia L.) callogenesis in indirect propagation systems, this work analyzed callogenic induction in adult leaves of walnut in broadleaf trees (BTM), Murashige and Skoog (MS) Driver Kuniyuki Walnut (DKW) and Woody Plant (WPM) media. Leaf segments of 1 cm 2 , previously sterilized, were placed in each culture medium, supplemented with 21.4 µM naphtalene acetic acid (NAA) and 8.8 µM 6-benzylaminopurine (BAP). A completely random design was used; the experimental unit was a Petri dish with six callus explants. Each treatment was composed of seven Petri dishes. Callogenesis (%), nodular calli (%) and histology of calli explants were evaluated at 4 weeks by staining 10 µm slices with safranine-fastgreen. There were no significant differences (α = 0.05) in callogenesis among evaluated media; but nodular calli percentages were significantly higher in BTM (75%) and WPM (63%). Only in calli obtained in the BTM medium did the histology show the presence of meristematic zones, but without external expression. These results indicate that the medium influences the morphogenic characteristics of resultant callus. Calli produced in BTM presented better morphogenic potential, with meristems at primary stages of organization.

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Section: Resultsmentioning

confidence: 99%

Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)

Avilés¹,

Ríos²,

González³

et al. 2009

Chilean J. Agric. Res.

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“…More rarely BAP alone or in combination with GA 3 was used (Q. petraea [20], Q. robur [5,34], Q. acutissima [39]). Zeatin alone or in combination with NAA was also used successfully in Q. robur [9].…”

Section: Introductionmentioning

confidence: 99%

“…Secondary embryogenesis on culture media without growth regulators has been reported for a number of species including Q. rubra [16], Q. suber [11,14], Q. acutissima [22] and Q. robur [9,34]. Fernández-Guijarro et al [14] showed that on these growth regulator-free media, the secondary embryogenesis is influenced by macronutrient composition.…”

Section: Introductionmentioning

confidence: 99%

Histological investigation of the multiplication step in secondary somatic embryogenesis of Quercus robur L.

2001

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-Standardized explants composed of hypocotyl and root-tip were prepared from embryonic structures obtained from one embryogenic line of Quercus robur L. maintained by regular transfer onto a solidified reference medium composed of the MS mineral solutions, glucose (0.12 M), casamino acid (0.1%), and NAA (10.74 µM). The regeneration capacity from these explants were tested on the reference medium and on 2 alternative media in which the NAA (10.74 µM) was omitted or substituted for a combination of IBA (9.80 µM)/BAP (8.90 µM). Within 30 days, 4 types of responses were observed including direct and indirect secondary embryogenesis. In the direct pathway, somatic embryos arose from 3-4 epidermal cells following two different modes, depending on whether or not the formation of a meristematic mass preceded the initiation of the embryogenic process. In the indirect pathway the embryos were formed from clumps of mitotically active cells included in callus developed within the cortical tissues. Depending on their histological origin, the embryos exhibited differences in their structural organization which could influence their potential for further maturation and conversion into viable plantlets. Explants prepared from small translucent embryonic structures were more embryogenic and expressed the direct pathway of secondary embryogenesis at higher frequency than explants prepared from more advanced embryonic structures. On the culture medium without growth regulator, direct secondary embryogenesis was the exclusive response whereas on the culture medium with growth regulators added both direct and indirect secondary embryogenesis occurred. NAA favoured the direct secondary embryogenesis, while conversely, the IBA/BAP combination stimulated the indirect secondary embryogenesis. The results are discussed in reference to the PEDC concept (pre-embryogenic determined cells).

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“…Plants in this stage are then ready for acclimatization (Pires et al, 2003;Figure 2a As reported above, somatic embryogenesis is a regeneration strategy with enormous potential for breeding programs. Somatic embryos were developed in Q. canariensis (Bueno et al, 1996;, Q. rubra (Vengadesan & Pijut, 2009), Q. serrata Takur et al, 1999), Q. robur (Cuenca et al, 1998;Endemann et al, 2002;Wilhelm et al, 1999), Q. acutissima (e.g., Kim, 2000) and Q. petrea (Chalupa, 2005). However, not only most studies use juvenile sources of explants (e.g., zygotic embryos and seedlings), but also plant conversion frequencies are still low, supporting the recalcitrance of these species.…”

Section: Quercus Suber In Vitro Cloning: a Reliable Protocol?mentioning

confidence: 99%

Somatic Embryogenesis and Cryopreservation in Forest Species: The Cork Oak Case Study

Santos¹

2012

Current Frontiers in Cryopreservation

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Somatic embryogenesis from stem and leaf explants of Quercus robur L.

Cited by 67 publications

References 0 publications

Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)

Effect of Culture Medium in Callogenesis from Adult Walnut Leaves (Juglans regia L.)

Histological investigation of the multiplication step in secondary somatic embryogenesis of Quercus robur L.

Somatic Embryogenesis and Cryopreservation in Forest Species: The Cork Oak Case Study

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